Bos taurus Olfactory Receptor Katie Davis 1,2 and Sandra Rodriguez-Zas 1 1 Department of Animal Sciences, University of Illinois Urbana-Champaign, 2 ACES.

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Presentation transcript:

Bos taurus Olfactory Receptor Katie Davis 1,2 and Sandra Rodriguez-Zas 1 1 Department of Animal Sciences, University of Illinois Urbana-Champaign, 2 ACES James Scholar Honors Program Introduction -Mammals have a very complex system from sensing environmental stimuli. Odorant molecules, in particular, are capable of providing a variety of information about the environment. -Olfactory receptors (OR) detect and identify a wide range of odors and chemosensory stimuli through the binding of and activation of the minimum of one olfactory receptor. -A large multigene family of olfactory receptor genes mediates odor perception in mammals. The amount of these genes varies between species. -A significant amount of homology exists between the genes coding for OR in Mus musculus, Homo sapiens and Canis lupus familiaris -M. musculus has approximately 1200 genes that code for OR, H. sapiens to have around 900 OR genes and C. lupus familiaris is estimated to have 800 OR genes. -By 2007 only 29 OR genes were reported in Bos taurus. Hypotheses -Based off the level of homology, a similar number of genes coding for OR should exist in Bos taurus -The homology between the species can be used to locate the remaining OR in Bos taurus. Objectives and Activities -The HomoloGene database automatically detects homologs that exist within the eukaryotic genomes that have been completely sequenced. This database was used to detect the homologs that exist within the olfactory receptors of the completely sequenced eukaryotic genomes. -A compilation of the known OR genes of M. musculus, H. sapiens and C. lupus familiaris and their related proteins was made using the Homologene, Protein, Nucleotide, and Gene databases in the NCBI GenBank This information was used to locate similar sequences in the B. taurus genome. Matches were identified based on a high percentage of sequence identities and similarities, a low number of gaps and the location of the matches in the genome. Matches between M. musculus sequences to fragments in the B. taurus genome were further investigated. a_F2.html Figure 1. Phylogenetic Tree, Figure 2. Sample Homologene Cattle Blast Match Table Results Genus species Olfactory Receptor Genes Studied with Homologene Olfactory Receptor Genes Discovered Using Cattle Blast Mus musculus 491N/A Homo Sapiens 328N/A Canis lupus familiaris 309N/A Bos taurus Figure 3. Table of OR genes studied Homologene Number Mus musculusOlfr1490 Mus musculus Protein ID NP_ Homo sapiensOR10W1 Homo sapiens Protein ID NP_ Canis lupus familiaris LOC Canis lupus familiaris Protein ID XP_ Blast Bos taurus Genome 8.00E-122 Features bp at 5' side: similar to olfactory receptor, family 1, subfamily S, member 1; bp at 3' side: similar to olfactory receptor 1487 Score Identities= 215/288 (74%), Positives=242/288 (84%), Gaps= 0/288 (0%), Frame=-2 StatusNew Conclusions Acknowledgements ACES James Scholar Honors Program -The known olfactory receptor for Mus musculus, Homo sapiens and Canis lupus familiaris were successfully extrapolated upon to locate new OR genes in the known Bos taurus genome. -The three-step process was a successful and time effective method for locating previously unknown olfactory receptor genes in the cattle genome. -The process also resulted in the compilation of data relating to all four species and their olfactory receptors. - Insight into distinguishing factors of the B. taurus olfactory receptor has the potential to positively impact the effectiveness of cattle production practices, and the overall well being of cattle in production settings.