Polymerase Chain Reaction (PCR): DNA Replication in vitro DNA replication in vivo DNA replication in vivo –Occurs in S-phase –Helicase, primers, DNA polymerase.

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Presentation transcript:

Polymerase Chain Reaction (PCR): DNA Replication in vitro DNA replication in vivo DNA replication in vivo –Occurs in S-phase –Helicase, primers, DNA polymerase PCR PCR –Replicates portions of DNA specified by primers –Thermal cycler, primers, DNA (Taq) polymerase, ATCG –Uses heating cycles to control DNA replication in vitro –2 n ; n = number of cycles –2, 4, 8, 16, 32, 64, 128, 256, 512, 1024, 2048,

Polymerase Chain Reaction (PCR): Applications Why: Why: –Forensics, Research, Paternity test, Medical screening When: When: –Small DNA sample –Check for presence of a gene location Must be used in conjunction with Electrophoresis Must be used in conjunction with Electrophoresis

PCR: What happens? Ingredients: Sample DNA, DNA (Taq) polymerase, primers, ATCG; Thermal cycler controls temperature. Ingredients: Sample DNA, DNA (Taq) polymerase, primers, ATCG; Thermal cycler controls temperature. Cooling allows primers to anneal Heating separates DNA strands DNA polymerase elongates new DNA chain Heat separates strands Primers anneal New chain is built REPEAT..