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Published byLoren Higgins
Modified over 5 years ago
POLYMERASE CHAIN REACTION (PCR)
THE TEMPLATE DNA GOES THROUGH A CYCLE OF HEATING AND COOLING TO FORM A NEW STRAND OF DNA THAT ONLY COMPRISES OF THE CANCEROUS DNA SEQUENCE THAT WE DESIRE.
THE DNA IS THEN RAISED TO NEAR BOILING, 95 DEGREES CELSIUS, TO SEPARATE INTO SINGLE STRANDS.
THE DNA IS THEN COOLED DOWN TO 55 DEGREES CELSIUS FOR THE FORWARD AND REVERSE PRIMERS TO ATTACH TO BOTH THE SIDES OF THE DESIRED DNA SEQUENCE.
THEN THE DNA STRANDS ARE HEATED TO 72 DEGREES CELSIUS FOR THE TAQ POLYMERASE TO ADD NUCLEOTIDES SO THE DNA CAN REPLICATE.
AFTER THE TAQ POLYMERASE DUPLICATES THE SEQUENCE WE NOW HAVE A DUPLICATION OF THE TEMPLATE DNA WITH ONLY THE DESIRED CANCEROUS SEQUENCE PRESENT.
THE PROCESS CYCLES THROUGH 35 TIMES SO THAT WE HAVE ENOUGH DNA CANCEROUS STRANDS FOR EXAMINATION.
ALL IMAGES CREDITED TO WWW.DNALC.ORG
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