The Compound Light Microscope

What is a compound light microscope? Mechanism for enlarging an image

Evolution of the microscope Choice of lens magnification 2 set lenses

The Parts of the Microscope and Their Function Maintains proper distance between lenses Magnification Rotates the objectives Support body tube Magnification Supports slide Holds slide In place Focuses image Regulates amount of light Sharpens the image Reflects light towards eyepiece Supports microscope

Parts of a Microscope Rotates the objectives Objectives, magnifies images a. Scanning (4X) b. Low Power (10X) c. High Power (40X) 3. Regulates the amount of light

Parts of a Microscope Lens that you look through (10X) Focuses the image Sharpens the image (only on high power)

Magnification The two lenses are called: Eyepiece Objective The eyepiece has a magnification of “10x” The magnification of the objective lenses vary and are marked on the lens Total magnification = (Eyepiece) X (Objective)

10X x = 40X 400X

10X 4X 40X 10X 10X 100X 10X 40X 400X Eyepiece Power Objective Power Total Magnification Scanning Low Power High Power 10X 4X 40X 10X 10X 100X 10X 40X 400X

How to make a wet-mount slide … 1 – Get a clean slide and coverslip from your teacher. 2 – Place ONE drop of water in the middle of the slide. Don’t use too much or the water will run off the edge and make a mess! 3 – Place the edge of the cover slip on one side of the water drop. 4 - Lower the cover slip on top of the drop to prevent air bubbles Cover Slip Lower slowly

Let’s Review! Eyepiece Body tube Nosepiece Arm Scanning Low Power 9. 10. 11. 14. Eyepiece 1. 2. 3. 4. 5. 6. 7. 8. Body tube Nosepiece Arm Scanning Low Power Stage High power Stage clips 12. Course adjustment Diaphragm 13. Fine adjustment Light source Base

So Let’s Review Let’s Review! Eyepiece Body tube Nosepiece Arm Scanning Low Power Stage High power Stage clips Course adjustment Diaphragm Fine adjustment Light source Base

Comparing Powers of Magnification We can see better details with higher the powers of magnification, but we cannot see as much of the image. Which of these images would be viewed at a higher power of magnification?

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