Unstoppable productivity; Uncompromised MS/MS simplicity Thermo Scientific TSQ 8000 Triple Quadrupole GC-MS/MS Unstoppable productivity; Uncompromised MS/MS simplicity

2 Triple Quadrupole GC-MS/MS is an essential part of a cost- effective, high productivity analytical method in today’s laboratory

3 Many laboratories are already investing in and exploring GC-MS/MS as a tool to obtain a competitive edge in their analyses

4 Routine laboratories face tough challenges High sample loads/short deadlines Keeping sample analysis costs down with more and more challenging LODs and matrices Integrating and maintaining new methods and technologies into production workflows to remain competitive

5 What‘s required.. Realization of the productivity advantages of high performance GC-MS/MS Minimizing the impact of adoption and implementation to current laboratory operations

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7 “To make the productivity advantages of high performance GC-MS/MS easy to achieve and available routinely; especially for high-throughput laboratories.” Design philosophy

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9 Routinely achieve regulatory detection limits with confidence Use highly powerful MS/MS to bring new capability Not just MS/MS performance High Sensitivity in MS mode for Full Scan/SIM work

10 Designed for more robustness; for more uptime and more results reported Powerful SRM enables optimized sample preparation for faster results Consolidated, faster methods with more compounds in one run

11 Fast and simple route to high performance MS/MS with integrated software tools like AutoSRM Automatically optimized instrument methods for maximum analysis sensitivty with t-SRM Synchronized instrument and data processing methods for maintaining efficient workflows

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13 Routinely achieve regulatory detection limits with confidence Full Scan SIM SRM Dichlorvos in mixed herbal extract at 5 ppb Cut through chemical backgroundCut through chemical background High selectivity in matrix compared with single stage MS Selected Reaction Monitoring (SRM) S-shaped curved ion guide lens High precision at low concentrationsHigh precision at low concentrations Femtogram level detection Confirming ions

14 Highly powerful MS/MS to bring new capability Low-level multi-drug class screen in oral fluid.. Cocaine & BZE at 0.8 ng/mL THC and THCA at 0.2 ng/mL (all with quantitative and confirmatory ions) Use the high speed & sensitivity of the TSQ™ 8000 GC-MS/MS Use the high speed & sensitivity of the TSQ™ 8000 GC-MS/MS For more compounds More confirmatory ions Achieve low noise and clean peak detectionAchieve low noise and clean peak detection Less manual integration changes Fast data processing Add versatility Add versatility Easy addition of new compounds to methods

15 Not just MS/MS performance High full scan spectral qualityHigh full scan spectral quality Searchable with NIST etc. High sensitivity SIM & Full ScanHigh sensitivity SIM & Full Scan Virtually indistinguishable from a single quadrupole instrument Dual acquisition modesDual acquisition modes Quan & qual in the same run Lindane isomers 40 ppb in cucumber in simultaneous full scan/SIM Full Scan SIM

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17 Designed for more robustness; for more uptime and more results reported EU limit for LOQ %RSD BeforeAfter %RSD pesticides (10 ppb) before and after 1,400 vegetable matrix injections Design based on strong reputationDesign based on strong reputation Thermo Scientific ISQ Single Quadrupole GC-MS Routine-grade robustnessRoutine-grade robustness Thermo Scientific ExtractaBrite ion source- no-vent maintenance Heated sample path zones for reduced ion burn Innovative GC modularity Innovative GC modularity Thermo Scientific TRACE 1300 Series GC Optimized maintenance

18 Powerful SRM enables optimized sample preparation for faster results Explore generic sample approaches Method consolidation Eg. QuEChERS Reduce number of stepsReduce number of steps Pre-concentration Clean-up Bring sample preparation onboard TSQ 8000Bring sample preparation onboard TSQ 8000 Thermo Scientific TriPlus RSH autosampler 1,3 –dichlorophenol, 1,4- dichlorophenol and 1,2- dichlorophenol (left to right) in ASE soil extract at 5 ppb.

19 Consolidated, faster methods with more compounds in one run Real example of method consolidation on the TSQ Five SIM methods combined to form a powerful MRM method for 350 pesticides (700 transitions) Method consolidationMethod consolidation Combine separate MS analyses Driven by selectivity of SRM Faster run timesFaster run times Compress chromatography Using MS/MS resolution Clean peak detectionClean peak detection Less manual integration changes Fast data processing

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21 A fast, simple route to high performance MS/MS AutoSRM Unknown SRM Transitions The very beginning or a SIM Method TSQ 8000 Inst. Method Known SRM Transitions From an existing method or SRM Transition list Thermo Scientific TraceFinder SW Optimized SRM Transitions From Compound Data Store or existing TSQ 8000 method Method Sync SRM Optimization Starting Point Integrated SRM management tools SRM creation and method management independent of your starting point

22 Fast,simple route to high performance SRM with AutoSRM 1) Precursor ion selection 2) Product ion selection 3) Collision energy optimization AutoSRMAutoSRM manages the SRM method development workflow From discovery through to final optimization

23 Automatically optimized methods for high sensitivity with t-SRM Retention timed-based acquisitionRetention timed-based acquisition Simple method management No more window calculations Timed-SRM (t-SRM)Timed-SRM (t-SRM) RT information from user or compound data store (CDS) Optimizes duty cycle Ensures maximum method sensitivity, precision and accuracy. Peaks never on the edge of windows Timed-SIM (t-SIM) also possibleTimed-SIM (t-SIM) also possible

24 Synchronized methods for maintaining efficient workflows MethodSyncMethodSync synchronizes acquisition and processing methods Exchange SRM and RT information Instrument method, TraceFinder™ SW CDS

25 Brilliant design, brilliant results Dual Filaments for Prolonged Uptime Heated ion guide protects main quadrupole sets so they never have to be cleaned or replaced. Curved ion guide enables off-axis optics to effectively eliminates neutral noise Choose between nitrogen or argon for collision gas. No expensive helium is required Full source removal without venting, including ion guide surface. No wire connections are necessary, and never vent to clean your instrument. Super fast analyzers switch between Full Scan, SIM and MRM in milliseconds.

26 Unstoppable productivity starts with TRACE™ 1300 Series GC Instant Connect (iC) injector and detector modulesInstant Connect (iC) injector and detector modules User switching in minutes For maintenance or flexibility Low thermal mass design throughoutLow thermal mass design throughout Fast heating and cooling for higher sample throughput TriPlus™ RSH autosamplerTriPlus™ RSH autosampler Automation of sample preparation steps Dilutions Addition of standards

27 TraceFinder; maximum productivity workflow-oriented software Workflow-centricWorkflow-centric Batch acquisition Integration to TSQ 8000 method Data processing User Role CapabilitiesUser Role Capabilities Simplifies routine workflow Active ReportingActive Reporting Dynamic updates based on real-time changes Application VariantsApplication Variants Environment & Food Safety Clinical Research & Toxicology

28 High performance Unstoppable productivity MS/MS simplicity TSQ 8000: Core values for the routine choice in GC-MS/MS

29 Your next competitive advantage