1. Egal Gorse 2006- 2007 Transfection of Recombinant Plasmid DNA From Immunotoxins into Eukaryotic Cells for the Development of an Antibody Targeted Cancer Therapy

2. Goals To determine if mammalian carrier cells would accept and express immunotoxin DNA. To evaluate which carrier cells most effectively expressed immunotoxin DNA.

3. Cancer Cancer is the leading cause of death in the world (7.6 million per year). There is no effective treatment for cancer. Chemotherapy is inefficient (resistance and not targeted). Immunotoxins have shown promise as an effective cancer therapy (Vallera 2005).

7. AT B Binding Inside Outside Golgi Apparatus Endoplasmic Reticulum How EpCAMKDEL Kills Cells Cytosol

8. AT B Inside Transport Vesicle Outside Golgi Apparatus Endoplasmic Reticulum How EpCAMKDEL Kills Cells

9. B A T Cytosol Inside Outside Endoplasmic Reticulum Golgi Apparatus Transport Vesicle How EpCAMKDEL Kills Cells

10. B T T A Cytosol Inside Outside Endoplasmic Reticulum Golgi Apparatus Transport Vesicle How EpCAMKDEL Kills Cells

11. B T T A Cytosol Inside Outside Endoplasmic Reticulum Golgi Apparatus Transport Vesicle How EpCAMKDEL Kills Cells

12. B T T A Golgi Apparatus Endoplasmic Reticulum Inside Outside Cytosol How EpCAMKDEL Kills Cells

13. T B Cytosol Golgi Apparatus Endoplasmic Reticulum Inside Outside How EpCAMKDEL Kills Cells

14. T A T A Cytosol Golgi Apparatus Endoplasmic Reticulum Inside Outside How EpCAMKDEL Kills Cells

15. Cytosol Transport Vesicle T A Endoplasmic Reticulum Inside Outside How EpCAMKDEL Kills Cells Golgi Apparatus

16. T A Cytosol Golgi Apparatus Endoplasmic Reticulum Inside Outside How EpCAMKDEL Kills Cells

17. T A Cytosol Golgi Apparatus Endoplasmic Reticulum Inside Outside How EpCAMKDEL Kills Cells

18. Conventional Immunotoxin Administration

19. Therapy Targeted Directly at The Site Of The Tumor Cells Transfected With Immunotoxins Directly at the Site of The Tumor

20. Transfecting EpCAMKDEL Immunotoxin DNA Into Cancerous Cells Using LNCX.NGFR

26. NIH-3T3 Transfected with EpCAMKDEL/LNCX.NGFR

27. Proliferation Assay

29. Conclusions Immunotoxin genes were capable of transfection into mammalian cells. The mammalian carrier cells were capable of expressing the immunotoxin genes. Expressed immunotoxins were capable of killing cancerous cells. The mouse cell lines tested were more capable of transfection than the human cell lines.

30. Future Study Testing in a live model (in vivo). Use results in an effective cancer therapy for human.

31. Acknowledgments Team Research Dr. Daniel Vallera Dr. Deborah Todhunter Ms. Lois Fruen

32. Egal Gorse 2006-2007 Transfection of Recombinant Plasmid DNA From Immunotoxins into Eukaryotic Cells for the Development of an Antibody Targeted Carcinoma Therapy