1. Subcutaneous mycoses (4) ZYCOMYCOSIS
Mrs. Dalia Kamal Eldien
Msc in Microbiology
Lecture NO (9)

2. Objectives The main subcutaneous fungal infections
Revision to Zygomycetes
Common causes of zygomycosis
Symptoms and clinical presntation of zygomycosis
Risk factors associated with zygomycosis
Laboratory diagnosis
Treatment

3. Introduction The main subcutaneous fungal infections include: Mycetoma
Chromoblastomycosis
Sporotrichosis
Lobomycosis
Rhinosporidiosis
Subcutaneous zygomycosis
Subcutaneous phaeohyphomycosis.

4. Zygomycetes
The zygomycetes are a relatively small group in the fungi kingdom and belong to the Phylum Zygomycota.
They include the familiar bread mold Rhizopus stolonifer, which rapidly propagates on the surfaces of breads, fruits, and vegetables.
They are mostly terrestrial in habitat, living in soil or on plants and animals.

5. a) white fuzz seen on this bread mold, Rhizopus stolonifer (b) tips of bread mold are the spore-containing sporangia.

6. Rotting of fruits

7. Zygomycetes
The Zygomycetes, a class of fungi with a ubiquitous and worldwide distribution that is characterized by aseptate hyphae, fast growing, saprophytic fungi.
The fungi usually reproduce asexually by producing sporangio spores .
When spores land on a suitable substrate, they germinate and produce a new mycelium.
Sexual reproduction starts when conditions become unfavorable, produce the zygospore

8. Sporingiospore

9. Zygomycosis
The term zygomycosis describes in the broadest sense to any infection due to a member of the Zygomycetes.
Zygomycosis is the third most common invasive fungal infection after candidiasis and aspergillosis
invasive fungal infections common immunocompromised individuals

10. This class of fungi encompasses two orders:
Mucorales
Entomophthorales

11. Mucorales The order Mucorales include several genera Rhizopus
Rhizomucor
Mucor
Absidia
Involved in rhinocerebral, pulmonary, cutaneous, gastrointestinal and other less frequent infections in immunocompromised individuals, and is characterized by a tendency to disseminate
Mucormycosis is the correct term for infections due to fungi of this order.
The term zygomycosis is used to describe any invasive infection due to zygomycetes, although it is frequently used interchangeably with the term mucormycosis

12. Entomophthorales The order Entomophthorales include Conidiobolus
Basiodobolus spp
Subcutaneous zygomycosis, which is typically seen in children and adolescents, results from infection with Basidiobolus haptosporus. 
It first develops as a localized lesion, usually on the thighs or buttocks, and it spreads slowly to form a hard, painless, non-pitting mass involving the cutaneous and subcutaneous tissues. The mass is shiny, but may later become ulcerated.

13. Symptoms of zygomycosis
The symptoms depend on where in the body the fungus is growing.
Mucormycosis most commonly affects the sinuses or lungs.
Symptoms of sinus infections include fever, headache, and sinus pain. Lung infections with the fungus can cause fever and cough.
Skin infections can develop after the fungus enters through a break in the skin caused by surgery, burns, or trauma. A skin infection can look like blisters or ulcers, and the infected tissue may turn black. Other symptoms of a skin infection include fever and tenderness pain, heat, excessive redness, or swelling around a wound.
If the infection is not treated quickly, the fungus can spread throughout the body, and the infection is often fatal.

14. Clinical presentation
Portals of entry of zygomycetes are usually the lungs, skin, and gastrointestinal tract.
These rare yet serious and potentially life -threatening fungal infections, usually affect the face or oropharyngeal cavity, lungs, gastrointestinal tract, skin, or less commonly other organ systems.
These types of infections are also common after natural disasters, such as tornadoes 
 A characteristic property of zygomycetes is their tendency to invade blood vessels and to cause thrombosis—processes that result in subsequent necrosis of involved tissues.

15. Risk factors associated with zygomycosis include:
Prolonged neutropenia
Use of corticosteroids
Solid organ or haematopoietic stem cell transplantation
AIDS
Poorly controlled diabetes mellitus
Burns& wounds
Malnutrition
Extremes of age
Intravenous drug

16. Laboratory diagnosis Specimen: Skin scrapings from cutaneous lesions
sputum and needle biopsies from pulmonary lesions
Nasal discharges, scrapings and aspirates from Sinuses in patients with rhinocerebral lesions
Biopsy tissue from patients with gastrointestinal and/or disseminated disease.

17. Direct Microscopy: 10% KOH & Parker ink or Calcofluor mounts for skin
Tissue sections should be stained with H&E.
Results: Examine specimens for broad, aseptate, thin-walled hyphae
As a rule, a positive direct microscopy, especially from a sterile site, should be considered significant.

18. Non septate hyphae of zygomycetes

19. Culture: 
Inoculate specimens onto Sabouraud's dextrose agar.
Most zygomycetes are sensitive to cycloheximide (actidione).
Colonial morphology:
Look for fast growing, white to grey or brownish, downy colonies.
Serology: 
Some laboratories have developed ELISA tests for the detection of antibodies to Zygomycetes.

20. Culture of zygomycetes

21. Treatment
 Treatment consists of prompt and intensive antifungal drug therapy and surgery to remove the infected tissue.
The prognosis varies vastly depending upon an individual patient's circumstances.

22. Reference
Source: Boundless. “Zygomycetes.” Boundless Microbiology. Boundless, 03 Jul Retrieved 08 Dec. 2014from 
Kwon-Chung KJ and JE Bennett Medical Mycology Lea & Febiger.