1. Innovative by Nature TM Dr. E.N. Zyme Biology AP, Monta Vista High School – September 13, 2013 Disclaimer: This presentation is used as a SIMULATION activity for high school students. The story is purely fictitious and is made for students to understand the real world applications for enzymes. However, student data will be reported to Genencor for review.

2. Genencor Today  Proven performer since 1982  Powerful, integrated technology – i-biotech  1500 Employees  Year 2000 - Public Offering NYSE ‘GCOR’  Corporate Offices - Palo Alto, Rochester NY, Leiden NETH.  Manufacturing - Argentina, US, Finland, Belgium, China

3. Genencor’s Vision  Leverage i-biotech platforms into multi-billion dollar opportunities  Expand presence in our target markets –Health Care –Agriculture –Industrial/consumer i-biotech – efficient discovery and delivery of safe and effective biomaterials

4. i-biotech in Everyday Life $300+ million revenues, $16+ million net income, 44% gross margins

5. Cleaning Products - Clothes/Dishes  Proteases –Removes protein stains (blood, grass, milk, tomato sauce)  Amylases –Removes starch stains (chocolate, gravy, rice, pasta)  Why? –Cleaner clothes/dishes, less chemicals, lower temperatures, enzymes are biodegradable

6. Textiles  Biofinishing –Cellulases for stonewashing and soft khakis  Desizing –Removal of starch using amylase during production  Why? –Cellulases are less harsh on fabric, no pumice stones –Starch initially protects textiles from breakage. Amylases are an easy way to remove them so that the fabric can be further treated.

7. Personal Care  Skin care –Enzymes can dramatically improve skin softness  Oral care –Enzymes can remove plaque  Hair care –Enzymes can remove dandruff LowGen™ enzymes will open new markets

8. Agriculture  Biomass into ethanol –Use enzymes to convert starch into sugars that can be fermented into ethanol by yeast  Animal feed and pet food –Enzymes can improve digestibility by increasing solubility of fibers and proteins  Production of high fructose corn syrup –Enzymes can convert starch into high fructose corn syrup (sweetener)

9. Other  Corn into ascorbic acid (Vitamin C)  Fruit juices  Paper recycling (cellulases)

10. Your research focus...   Optisize COOL –Amylase enzyme –Genencor Textile Finishing Enzyme  Possible uses on fabric: –Desizing Removes starch from fabricsRemoves starch from fabrics

11. Your task…  Test Optisize COOL at a broad ranges of temperatures and pH’s to determine its usefulness. –Customers will want to know effectiveness of enzyme at various pH’s and temperatures  Now…review the lab procedure!

12. Overview of Procedure  Amylazyme Tablet –Substrate for the experiment –Blue dye crosslinked with amylose (starch)  How will you know how well the enzyme is working? –The more dye released = more enzyme activity  Buffers (with enzyme) at 6 different pHs and 7 temperatures will be tested (equilibrate to get it to the right temperature)  Each reaction will run for exactly 10 minutes –Stop reaction after 10 minutes by adding Stop Solution (Trizma Base) –This denatures the enzyme

13. Overview of the Procedure  Why filter? –Gets rid of the chunks of tablets that did not get reacted/broken down  How to measure “how blue” it is? –Spec20 –Absorbance = how much light is being absorbed by the dye in the solution –Higher absorbance = higher enzyme activity