1. Introduction to training
Agenda
Product overview
What’s new from ProStar 325
Hardware overview
Initialization routine
Software CD overview
Detector installation & PolyVerfiy
PolyDiagnostics

2. Product Overview
Based on the ProStar 325 with many shared parts
Two models available:
Single 1024 array
Dual 1024 array
Control by both Star with PolyView 2000 and Galaxie (PolyView is now included with Star driver)

3. Product Overview - workstation control
Galaxie
Four 335 DADs per acquisition server
Driver included in Varian LC control drivers
Star
two 335 DADs per Star Workstation
Driver included in LC control option
Installation of driver automatically installs PolyView 2000
Ethernet communications best with a switch (not a hub)

4. ProStar 335 and 325 similarities
Product Overview
ProStar 335 and 325 similarities
Same cabinet
Same UV & visible lamps
Same flow cells
Same rear panel, connectors, interface boards & cables
Same communications protocol
Same power supply
Same Main pwb (difference in firmware)

5. What’s New
Same look as ProStar 325 except no PPC capabilities

6. Different optics module
What’s New
Different optics module
Dual Array
1024 photodiode sample and reference array
Extended range spectra
Used for Analytical to Prep Applications
Single Array
1024 photodiode sample array
2 photodiode references (250 and 500 nm)
No extended range
Uses 9x0 and 4x0 flow cells only

7. Hardware Overview – optics module

8. Product Overview – ray trace diagram

9. Hardware Overview - Polychromator
Available slit widths
1nm 2nm 4nm 8nm 16nm

10. Hardware Overview – dual path flowcell
Illustration is of a 9x1 flowcell
reference
sample

11. Hardware Overview – optics module
Wavelength calibration data must be entered into the EEPROM using Diagnostics software.

12. Electronics – wavelength calibration factors
Use the floppy disk to update the EEPROM automatically
This label will be attached to the top of each optics module

13. Hardware Overview – Electronics
Only difference between 335 & 325 is firmware

14. Hardware Overview - Electronics
Add new MAC address label when swapping pwb’s
New MAC address label comes with each new PWB

15. Hardware Overview – initialization routine
Peak search for 656.1nm deuterium emission line
Determines dark current table (0%T)
Gain (Lamp) calibration is now user driven. Stored in non-volatile memory.

16. Initialization
Hardware fault
initialized
Status LED’s
not initialized

17. Software CD overview
Software CD ships with each ProStar 335 detector.
Part number:
Auto-loading CD which contains:
Galaxie drivers and manuals
Star driver and manuals
PolyVerfiy software
PolyDiagnostics

18. Customer software CD 85-1xxxxx-00 will contain
Software CD overview
Customer software CD 85-1xxxxx-00 will contain

19. Software CD overview – installation PolyVerify
After installing, a desktop icon will be created called ProStar
Use PolyVerify to check the performance of the ProStar 335

20. Software CD overview - installation PolyVerify
Only two test are required at installation;
Noise/Drift
Wavelength accuracy
IQ/OQ is also available part number: ;4

21. Software CD overview – installation PolyVerify
Print customer certificate

22. The ProStar 335 must be initialized and have both lamps on for at least one hour prior to running any tests.
Noise test : pump water for at least 10 minutes at a pump head pressure of at least 100 atm using a back pressure restrictor of 40psi.
Wavelength accuracy: same conditions as the Noise test.

23. Software CD overview - PolyDiagnostics
Installing PolyDiagnostics
Browse the CD using Windows Explorer.
Diagnostics is not self loading and not intended for customer use.

24. Software CD overview - PolyDiagnostics
To run PolyDiagnostics you will need to copy these three files to the ProStar directory where PolyVerify is installed. From there double click the “PolyDiagnostics.exe” file using Windows Explorer.

25. Software CD overview - PolyDiagnostics
Similar appearance to 325 Diagnostics

26. Software CD - PolyDiagnostics
Use the Diagnostics program to update the EEPROM and perform various calibration routines i.e. wavelength & lamp calibrations
Service folder also contains the firmware updater program

27. Software CD - PolyDiagnostics
The EEPROM contents will need updating whenever;
the Main PWB is replaced
the optics module is replaced
to update installation & maintenance dates

28. Software CD - PolyDiagnostics
EEPROM contents;
Detector serial number
MAC address
Wavelength calibration factors, slope and offset
IP address
Gateway address
Subnet mask
Lamp timer (on/off date and times)
Detector installation date
Last maintenance date
Error log
Hg wavelength calibration date

29. Software CD - PolyDiagnostics
When to calibrate
Performing a Lamp Calibration will help reduce noise and improve linearity.
Deuteriunm lamp is changed
Visible lamp is changed
Every 1000 lamp source hours
Increasing signal noise
When changing flowcell to one of a different pathlength