1. CLS 311 Basic Microbiology
INSTRUCTOR
Mrs. Ohoud Alhumaidan

2. Burton's Microbiology for the Health Sciences
Reference :
Burton's Microbiology for the Health Sciences
By Paul G Engelkirk, Janet Duben-Engelkirk –
Lippincott Williams & Wilkins (2010), Ninth
Edition.

3. Microbiology Microorganisms are EITHER Pathogens OR Non-pathogens.
Microbiology  is the study of very small living organisms
Microorganisms are EITHER
Pathogens OR Non-pathogens.

4. Why Should We Study Microbiology?
Microorganisms living on and inside us are 10 times more than the no. of our cells. These microorganisms are called Normal Flora ( indigenous microflora)
opportunistic pathogens ??
Microorganisms are part of the food chain as tiny animals feed on them. Others are involved in elemental cycles like carbon, nitrogen, sulfur

5. Why Should We Study Microbiology?
Many microorganisms are essential in various food and beverage industries
Some bacteria and fungi used to produce antibiotics
Microorganisms are essential in the field of genetic engineering

6. Pioneers in the Science of Microbiology
Antoni van Leeuwenhoek.
Louis Pasteur.
Robert Koch.

7. Antoni van Leeuwenhoek (Holland 1632-1723)
The father of microbiology: he is the first one to see living bacteria and protozoa.
He was the first to create what is known today as “single lens microscope” by grinding tiny glass lenses and putting them in metal frames.

8. “single lens microscope

9. Louis Pasteur (France 1822-1895)

10. Louis Pasteur (France 1822-1895)
He discovered the process of alcohol fermentation.
Developed a process called Pasteurization .
He discovered forms of life that can exist in the presence of oxygen called “aerobes” and ones that can exist in the absence of oxygen “anaerobes”

11. Louis Pasteur (France 1822-1895)
4. He discovered the infectious agent that affect silk industry.
5. He made significant contribution to the germ
theory of disease
5. He developed vaccine for dog and human rabies.

12. Robert Koch (Germany 1843-1910)
He made significant contribution to the
germ theory of disease.
He developed methods of fixing, staining,
and photographing bacteria.
He developed methods for culturing bacteria on solid media.
4. He discovered the bacterium (Mycobacterium tuberculosis) that cause tuberculosis and Invented skin test to diagnose the Tb.
5. He discovered the bacterium (Vibrio cholerae) that causes cholera.

13. Classification of Microorganism
Cellular
Acellular
Prokaryotes
Eukaryotes
ex. Viruses.
ex. Algae, protozoa, fungi, plants, animals, and humans.
ex. Bacteria

14. Prokaryotic Cell & Eukaryotic Cell

16. Comparison between Eukaryotic and prokaryotic
Prokaryotic Cell
Eukaryotic Cell
Plant animal
Biological distribution
Nuclear Membrane
Membranous structures other than cell membrane
Cytoplasmic ribosome's (density)
Cell wall
chromosomes

17. Taxonomy Classification : Kingdom (5 major divisions)
Taxonomy is the science of classifying living organisms.
Classification :
The arrangement of organisms into taxonomic groups (taxa) on the basis of similarities or relationships.
The taxa include:
Kingdom (5 major divisions)
Phylum (groups of related Classes)
Class (groups of related Orders)
Order (groups of related Families)
Family (groups of related Genera)
Genus (groups of related Species)
Species (living organisms that are alike)

18. The Five Kingdoms
1. Monera
(unicellular prokaryotes: bacteria, cyanobacteria, blue-green algae
2. Protista
(unicellular eukaryotes: protozoa,
unicellular algae, slime molds)
3. Fungi
(multicellular eukaryotes: molds, mushrooms, yeasts)
4. Plantae
(multicellular eukaryotes: plants)
5. Animalia
(multicellular eukaryotes: animals)

19. Relationships between Organisms
Symbiosis
Permanent association between two different organisms.
There are several kinds of symbiosis
Neutralism
Two organisms living together, and neither is affected by that.
Commensalism:
Two organisms living together, one is benefited and the other is not been affected.
Mutualism
Two organisms living together, and both benefit from that.
Parasitism:
Two organisms living together, one is benefited ‘’called parasite’’ and the other is harmed ‘’called host’’.
Synergism:
Sometimes, two (or more) microorganism may work together “team up” to produce a disease that neither could cause by itself.