1. Photosynthesis: The Lab Dr. Ippolito

2. Background

3. (see last 2 lectures…)

4. Materials (Including the lab Manual) Part 1: –2 test tubes, water, phenol red, a straw, a 2” piece of Elodea Part 2: –test tube+cork; mortar and pestle; leaf; acetone; spot plate; filter paper; organic solvent Part 3: –A variegated leaf; Hot water bath; hot alcohol; petri dish; iodine; Part 4: –4 test tubes (A-D); P0 4 buffer pH 6.5; chloroplasts; DCPIP (strong redox potential!); aluminum foil; light source Part 5: –Microscope; prepared slides ONE, TWO, THREE

5. Overview: What we’re doing Part 1: Watch photosynthesis happen in a closed system. Part 2: Separate the color molecules in a leaf. Part 3: Show how only the green parts of a plant make sugar. Part 4: Show how the ETC in the light reactions of chloroplasts has NOTHING to do with carbon fixation of the Calvin Cycle Part 5: look at the structural differences between C3 plants and C4 plants.

6. Setup Organize in groups of 2-3. There are 10 setups total. Read through the materials and make sure you have them all. Try to work together on one part at a time. Be careful with some of the reagents. They are slightly dangerous. Wear protective gloves and goggles when dealing with organic setups.

7. Time Considerations You will have the entire class period today to work on the entire lab. Work cautiously. Do not rush. You will be expected to stay the entire class period –this discourages groups to rush through their work so they can leave early.

8. Time Considerations Part 1: Start it and come back to it in 45’ to an hour. Part 2: Can be done all at once. –I will determine whether we are calculating Rf values. Part 3: Reread page 8, the starch test. Can then be done all at once.

9. Time Considerations Part 4: This will be the time-consuming portion. –Observations at 5 minute intervals. Use a clean piece of paper and record 5 intervals total. Record any notes that differ from the standard protocol. Part 5: Do NOT take out your microscope until you have a clean bench and hands. I will check over your lab space before I give you slides. Please do NOT use your gloves from previous sections when handling the microscopes. –This part can be worked through quietly, while reading along in your laboratory manual.

10. Assessment Expectations You and your group are expected to complete the entire laboratory. Incomplete sections will negatively affect your laboratory grade. Each group will turn in ONE set of pages 63 – 72. –Answer questions on pages 64, 66, 68, 69 Each STUDENT will turn in on CLEAN PAPER –Diagrams on page 67, 70, 71 (two diagrams) –A single paragraph hypothesis regarding guard cells (page 72 question #1) –A 3-5 sentence paragraph answering question #2 on page 72. The Group Lab Report will be stapled together. Individual Group Members work will be paperclipped to the stapled report. I will expect this work to be neat and typed out in 10-12 point Arial font, double spaced. This will be due November 20 th, the last day before Thanksgiving break. Late lab reports will not be accepted.

11. Time Considerations This powerpoint will stay up during the lab; feel free to come up and look through it at any time, or ask me for clarifications. Please note this is my first run through this laboratory. It will go smoothly, but expect the unexpected!