1. Antibacterial activity of antiseptics and disinfectants
Banea Ioana

2. Introduction
within hospitals, a proper decontamination of surfaces and hands of the staff is mandatory, considering the need of nosocomial infections prevention
Disinfectants – strong bactericidal activity
Used on surfaces
Antiseptics – low bactericidal/bacteriostatic activity
Used on hands, lesions

3. Purpose
The comparison of antibacterial activity of various antiseptics and disinfectants that are commonly used in hospitals

4. Materials and methods we analysed the activity of
four type of antiseptics
Clorhexin
Pliwa derm
Iodine
Alcohol
four disinfectants
Triton rapid
Pliwa big extra
Roditzin
Pliwa lemon fresh
On seven different bacterial species

5. Serial binary dilutions
Materials and methods
Serial binary dilutions
(100 μl)
Decontaminants
(200 μl)
standard CLSI microdilution method.
In microtiter plates, binary dilutions were made of each decontaminant to a final volume of 100 μl.
0.5 McFarland bacterial suspensions
10 μl were inoculated in μl Muller-Hinton broth.
100 μl were inoculated into each of the plate wells.
incubated at 35°C for 24 hours.
100 μl
0.5 McFarland
9990 μl M-H
10 μl

6. Materials and methods
For each bacterial species and for each decontaminant, the last dilution were no growth was found was noted.
MIC

7. Materials and methods
From the last three dilutions without growth > spot inoculation
MBC

8. Results MIC dilution factors
S. aureus
E. faecalis
E. coli
P. aeruginosa
Shigella
Salmonella
Clostridium
Clorhexin 32 4 16 2
Pliwa derm 8 64
Iodine
128
256
Alcohol
Triton rapid
4096
Pliwa big extra
512
Roditzin
Pliwa lemon fresh
Antiseptics
Disinfectants
The highest the dilution factor …
The strongest the decontaminant
More sensitive bacteria

9. Results Potency rank of the decontaminants
Name
Type
Component 1
Component 2
Componenet 3 1
Pliwa big extra
disinfectant
amine
Quaternary ammonium salt
polyether 2
Triton rapid
polieter 3
Pliwa lemon fresh
alcohol 4
Iod
antiseptic
iodine 5
Roditzin
alcohol, phosphates
enzymes, surfactants 6
Pliwa derm 7
Clorhexin
clorhexidin digluconate 8
Alcool
Potency

10. ResultS Efficiency of decontaminants
Alcohol - based
Amine & Quaternary ammonium - based

11. Results High Bacterial resistance
To disinfectants
Pseudomonas aeruginosa
Escherichia coli
To antiseptics
Shigella
Salmonella
Gram-negative bacteria

12. Conclusions The disinfectants have strong antibacterial activity
there are some bacterial species which resist better than other, especially if these substances are diluted too much.
The non-alcohol based decontaminants are the most potent
For the decontamination of skin, other antiseptics than alcohol are recommended.
The most resistant bacteria are the Gram-negative

13. Thank you for attention