1. Bacterial Transformation Teacher Preparation Sterile Materials

2. Sterile Materials Paper Clips, Glass Pipettes and Toothpicks Heat the foil packages containing paper clips, glass pipettes, and toothpicks at 350 º F for 30 minutes to sterilize. Bacterial Transformation Teacher Preparation

3. Sterile Materials 1.5 ml Tubes Sterilize 6- 1.5 ml microcentrifuge tubes for each group of students. Wrap the tubes in aluminum foil and heat at 250 º F for 30 minutes. Bacterial Transformation Teacher Preparation

4. Sterile Materials Distilled Water In a bottle with a cap, add 50 ml of distilled water. Cap the bottle and place it in boiling water for 30 minutes. Refrigerate to prevent contamination. Bacterial Transformation Teacher Preparation

5. Sterile Materials CaCl 2 Solution Step 1 Dissolve 0.75 g of CaCl 2 into 50 ml of distilled water in a labeled 100-ml glass bottle with a cap. Bacterial Transformation Teacher Preparation

6. Sterile Materials CaCl 2 Solution Step 2 Cap the bottle and place it in boiling water for 30 minutes. Refrigerate to prevent contamination. Bacterial Transformation Teacher Preparation

7. Sterile Materials Ampicillin Solution Add 1 ml of sterile distilled water to a 50 mg tube of ampicillin powder (provided to Iowa teachers by the Office of Biotechnology). Bacterial Transformation Teacher Preparation