1. Bacterial Transformation Teacher Preparation Materials Lists

2. Materials List-Item 1 (per group of students) 1 aluminum foil packet containing 4 sterile paper clips that are large and smooth. The clips should be opened into a 90° angle and the small end bent to close it. Bacterial Transformation Teacher Preparation

3. Materials List -Item 2 (per group of students) 1 aluminum foil packet containing 4 sterile paper clips that are small and smooth. Bend one end of each paper clip into a loop. Bacterial Transformation Teacher Preparation

4. Materials List -Item 3 (per group of students) 2 microcentrifuge tubes (1.5 ml) containing 2 drops of sterile CaCl 2 and labeled "CaCl 2 " Place on ice in a refrigerator until ready to use. Bacterial Transformation Teacher Preparation

5. Materials List -Item 4 (per group of students) 1 petri dish containing only nutrient agar and 1 dish with ampicillin labeled "No Amp" on the bottom Bacterial Transformation Teacher Preparation

6. Materials List -Item 5 (per group of students) 1 petri dish containing nutrient agar and 1 dish with antibiotic ampicillin labeled "Amp" on the bottom Bacterial Transformation Teacher Preparation

7. Materials List -Item 6 (per group of students) Copies of the laboratory instructions, one for each student Bacterial Transformation Teacher Preparation

8. Materials List -Item 7 (per group of students) 1 glass bottle with a cap containing sterile nutrient broth and labeled "LB Broth" Bacterial Transformation Teacher Preparation

9. Materials List -Items 8 & 9 (per group of students) 1 Sharpie marking pen 1 rubber bulb for the pipettes Bacterial Transformation Teacher Preparation

10. Materials List -Item 10 (per group of students) 1 aluminum foil packet containing the glass parts of 4 sterile pasteur pipettes or sterile plastic pipettes Bacterial Transformation Teacher Preparation

11. Materials List -Item 11 (per group of students) 1 aluminum foil packet containing 8 sterile toothpicks Bacterial Transformation Teacher Preparation