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Electrophoretic coating of amphiphilic chitosan colloids on regulating cellular behaviour by Yen-Jen Wang, Teng-Yuan Lo, Chieh-Hsi Wu, and Dean-Mo Liu.

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Presentation on theme: "Electrophoretic coating of amphiphilic chitosan colloids on regulating cellular behaviour by Yen-Jen Wang, Teng-Yuan Lo, Chieh-Hsi Wu, and Dean-Mo Liu."— Presentation transcript:

1 Electrophoretic coating of amphiphilic chitosan colloids on regulating cellular behaviour by Yen-Jen Wang, Teng-Yuan Lo, Chieh-Hsi Wu, and Dean-Mo Liu Interface Volume 10(86):20130411 September 6, 2013 ©2013 by The Royal Society

2 SEM images and AFM images of functionalized substrates: (a) bare stainless steel; (b) sharp surfaces (high peak-to-valley texture, termed CHC-1); (c) flat surfaces (low peak-to-valley surfaces, termed CHC-5). Yen-Jen Wang et al. J. R. Soc. Interface 2013;10:20130411 ©2013 by The Royal Society

3 SEM images of the contact phenomenon of cells cultured on CHC-1 (sharp surfaces) (a) and CHC-5 (flat surfaces) (b) in response to different culturing times. Yen-Jen Wang et al. J. R. Soc. Interface 2013;10:20130411 ©2013 by The Royal Society

4 The relationship between (a) the VSMC attachment numbers and (b) average viability of the attached VSMCs, at the short period of culture time (0.5, 1, 2 and 4 h) on various functionalized substrates (bare stainless steel, CHC-1 and CHC-5). Yen-Jen Wang et al. J. R. Soc. Interface 2013;10:20130411 ©2013 by The Royal Society

5 Laser scanning confocal microscope images of the cytoskeleton and nuclei of VSMCs cultured on functionalized substrates: (a) bare stainless steel, (b) CHC-1 (sharp surfaces), (c) CHC-5 (flat surfaces). Yen-Jen Wang et al. J. R. Soc. Interface 2013;10:20130411 ©2013 by The Royal Society

6 The relationship between the viability of (a) HUVECs and (b) VSMCs; the proliferation of (c) HUVECs and (d) VSMCs, at the long period of cultured time (24, 48 and 72 h) on the bare stainless steel, CHC-1 and CHC-5. Yen-Jen Wang et al. J. R. Soc. Interface 2013;10:20130411 ©2013 by The Royal Society

7 Biochemical analyses of PI3K on the various functionalized substrates. Yen-Jen Wang et al. J. R. Soc. Interface 2013;10:20130411 ©2013 by The Royal Society


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