1. Day 1: TWIN-SHIME inoculation with the same faecal sample. Days 1- 22: Stabilitation period. Days 1t- 11t: Treatment period. Supplementation of Provinols ® and Provinols ® + L. plantarum IFPL935 Day 12: End of supplementation. Days 12-20: Washout period. E. Barroso 1*, T. van de Wiele 2, A. Jiménez-Girón 1, I. Muñoz-González 1, M.V. Moreno-Arribas 1, B. Bartolomé 1, C. Peláez 1, M.C Martínez-Cuesta 1, T. Requena 1 Lactobacillus plantarum IFPL935 improves colonic metabolism in a simulator of the human intestinal microbial ecosystem during feeding with red wine phenolic compounds 1 Instituto de Investigación en Ciencias de la Alimentación CIAL (CSIC-UAM), Madrid, Spain. 2 LabMET, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium. *elvira.barroso@csic.es In the present work we have evaluated the adaptation of the colonic microbiota developed in two parallel five-stage in vitro gastrointestinal simulators (TWIN-SHIME) to long-term feeding with a polyphenol-rich red wine extract and the combination of this extract with a potential probiotic strain Lactobacillus plantarum IFPL935. Days Butyrate (mg/mL) ACTCDC (+) – catechin 4-hydroxy-5-(3’,4’-dihydroxyphenyl)- valeric acid 1-(3’,4’-dihydroxyphenyl)-3-(2’’,4’’,6’’-trihydroxyphenyl)- propan-2-ol 4-Hydroxy-5-(phenyl)-valeric acid L. plantarum IFPL935 (Tabasco et al., 2011) Control: pH, Tª, feed Experimental period The polyphenolic extract addition (day 1t) caused a sharp reduction of the fermentation activity (SCFAs) in the three colon vessels. Significant increase of butyrate production during the polyphenols treatment and the washout period in vessels TC and DC treated with L. plantarum IFPL935 compared with the vessels without the strain. BUTYRATE PRODUCTION This work was supported by Research Projects AGL2009-13361-C02-00, RM2011-00003-00-00 and Consolider Ingenio 2010 FUN-C-FOOD-CSD2007-00063, and program ALIBIRD P2009/AGR-1469. MICROBIAL CATABOLISM OF WINE POLYPHENOLS Catabolism of (+)-catechin. The evolution of the content of (+)-catechin during the treatment and washout periods shows disappearance of this compound in TC and DC, in accordance to the progressive appearance of phenolic metabolites starting after 4-5 days of treatment. L. plantarum tend to favor the catabolism of (+)-catechin, showing a more rapid disappearance of the precursor to the formation of further-intermediate products. A decrease in the microbial community is observed the first days of treatment, related with the reduction of butyrate and total SCFAs (data not shown). The increase of butyrate was associated with a faster recovery of the butyrate-producing bacteria, Clostridium leptum, Clostridium coccoides-Eubacterium rectale group and Ruminococcus on TC and DC vessels in the SHIME supplemented with L. plantarum IFPL935. Conclusion L. plantarum IFPL935 shows a protective effect on butyrate-producing bacteria and is able to improve colonic metabolism by promoting the formation of biologically significant compounds Conclusion L. plantarum IFPL935 shows a protective effect on butyrate-producing bacteria and is able to improve colonic metabolism by promoting the formation of biologically significant compounds MICROBIAL COUNTS by qPCR (counts are expressed in average log copy number/mL) MICROBIAL COUNTS by qPCR (counts are expressed in average log copy number/mL) Provinols ® Provinols ® + L. plantarum IFPL935 Provinols ® Provinols ® + L. plantarum IFPL935 Reference: Tabasco, R., Sánchez-Patán, F., Monagas, M., Bartolomé, B., Moreno-Arribas, M.V., Peláez, C., Requena, T. 2011 Effect of grape polyphenols on lactic acid bacteria and bifidobacteria growth:resistance and metabolism. Food Microbiol. 28: 1345-1352.