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Het-genes in Aspergillus niger Károly Pál János Varga Fons Debets Laboratory of Genetics Wageningen University 2002-2006.

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Presentation on theme: "Het-genes in Aspergillus niger Károly Pál János Varga Fons Debets Laboratory of Genetics Wageningen University 2002-2006."— Presentation transcript:

1 het-genes in Aspergillus niger Károly Pál János Varga Fons Debets Laboratory of Genetics Wageningen University

2 I. Introduction: - occurence and role of het-genes - het-genes in the Aspergillus genus II. Search for and comparison of het-genes and proteins - search for het- and incompatibility related genes in filamentous fungi and yeast - comparison of HET- and incompatibility related proteins between two A. nigers III. Conclusions – future studies

3 I. Introduction: occurence and role of het-genes

4 Life cycle of Aspergillus (Emericella) nidulans

5

6 Importance of incompatibility: preserving fungal individuality avoiding infectious genetic elements (mycoviruses, transposons) avoiding resource exploitation

7 Heterokaryon incompatibility is well studied in Neurospora crassa and Podospora anserina, whereas cell death processes are investigated in Saccharomyces cerevisiae.

8 I. Introduction: het-genes in the Aspergillus genus Most of the known Aspergillus species are asexual, only the A. nidulans was analyzed until now for the presence of het-genes. Natural A. niger isolates are highly incompatible with each other. Genome sequencing projects facilitate research, het- and incompatibility related gene homologs were found in the genome sequence of A. fumigatus A. oryzae A. terreus and A. niger

9 II. Search for and comparison of het-genes and proteins

10 II. Search for and comparison of het-genes and proteins: search for het-genes in filamentous fungi and yeast Genomes of 8 fungal species were scanned: A. fumigatus A. nidulans A. niger (CBS and ATCC1015) A. oryzae A. terreus N. crassa P. anserina S. cerevisiae

11 A. fumigatus 2 - A. nidulans 3 - A. niger (CBS513.88) 4 - A. niger (ATCC1015) 5 - A. oryzae 6 - A. terreus 7 - N. crassa 8 - P. anserina 9 - S. cerevisiae

12 II. Search for and comparison of het-genes and proteins: comparison of HET- and incompatibility related proteins between two A. nigers

13 The A. niger HET-C protein was analyzed in details. - already investigated in details in N. crassa (3 alleles) and P. anserina (no allelism) - in A. niger: different length of glutamine repeats: why? CBS FGAAGHSHGSQQQRPPQQQ----QQQQQQQQ---GSSPLPWDKLSSLPIP Ned FGAAGHSHGSQQQRPPQQQ----QQQQQQQQQQQGSSPLPWDKLSSLPIP Ind FGAAGHSHGSQQQRPPQQQ----QQQQQQ-----GSSPLPWDKLSSLPIP N402 1 FGAAGHSHGSQQQRPPQQQ----QQQQQQQ----GSSPLPWDKLSSLPIP Ned FGAAGHSHGSQQQRPPQQQ----QQQQQQQ----GSSPLPWDKLSSLPIP Bra3.1 1 FGAAGHSHVSQQQRPPQQQ----QQQQ GSSPLPWDKLSSLPIP Gu1.1 1 FSAAGHSHGSQQQRPPQQQ----QQQQQQ-----GSSPLPWDKLSSLPIP Bra2.2 1 FSAAGHSHGSQQQRPPQQQRPPQQQQQQQ-----GSSPLPWDKLSSLPIP Ind FSAAGHSHGSQQQRPPQQQ----QRSQQQ-----GSSPLPWDKLSSLPIP Bra2.5 1 FSAAGHSHGSQQQRPPQQQ----Q GSSPLPWDKLSSLPIP consensus 1 *.******.********** *..... ****************

14 The A. niger HET-C protein was analyzed in details. - already investigated in details in N. crassa and P. anserina - different length of glutamine repeats: why? CBS FGAAGHSHGSQQQRPPQQQ----QQQQQQQQ---GSSPLPWDKLSSLPIP Ned FGAAGHSHGSQQQRPPQQQ----QQQQQQQQQQQGSSPLPWDKLSSLPIP Ind FGAAGHSHGSQQQRPPQQQ----QQQQQQ-----GSSPLPWDKLSSLPIP N402 1 FGAAGHSHGSQQQRPPQQQ----QQQQQQQ----GSSPLPWDKLSSLPIP Ned FGAAGHSHGSQQQRPPQQQ----QQQQQQQ----GSSPLPWDKLSSLPIP Bra3.1 1 FGAAGHSHVSQQQRPPQQQ----QQQQ GSSPLPWDKLSSLPIP Gu1.1 1 FSAAGHSHGSQQQRPPQQQ----QQQQQQ-----GSSPLPWDKLSSLPIP Bra2.2 1 FSAAGHSHGSQQQRPPQQQRPPQQQQQQQ-----GSSPLPWDKLSSLPIP Ind FSAAGHSHGSQQQRPPQQQ----QRSQQQ-----GSSPLPWDKLSSLPIP Bra2.5 1 FSAAGHSHGSQQQRPPQQQ----Q GSSPLPWDKLSSLPIP consensus 1 *.******.********** *..... ****************

15 III. Conclusions - most of the het-, incompatibility related and PCD homolog genes are present in the Aspergilli including the A. niger - based on the genome sequences with molecular genetic tools now we can analyze functionality of these genes (i.e. transformations, deletions) - genome sequences can help us to reveal the secrets of incompatibility in A. niger


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