1. LB145 Sec 001-004 Happy Valentine’s Day!

2. Today’s Announcements StudyNotes5 AND Homework4 are due on Tuesday Feb 19 th. **Download HW VERSION 2.0** Field Trip sign up – started! Glycolysis and the Krebs Cycle. Oxidative Phosphorylation, the ETC.

6. Glycolysis: What do you need to know? Track the Carbons and Phosphates: Glucose + ATP  Fructose-P + ADP Fructose-P + ATP  P-Fructose-P P-Fructose-P  P-CCC + P-CCC P-CCC + P-CCC + 2NAD + + 2P i  P-CCC-P + P-CCC-P + 2NADH P-CCC-P + P-CCC-P + 2ADP  P-CCC + P-CCC + 2ATP P-CCC + P-CCC + 2ADP  Pyruvate + Pyruvate + 2ATP

7. Methods of Producing ATP GLYCOLYSIS & CITRIC ACID CYCLE Substrate-level phosphorylation occurs when ATP is produced by the enzyme-catalyzed transfer of a phosphate group from an intermediate substrate to ADP.

9. Campbell: Fig. 9-6-3 Mitochondrion Substrate-level phosphorylation ATP Cytosol Glucose Pyruvate Glycolysis Electrons carried via NADH Substrate-level phosphorylation ATP Electrons carried via NADH and FADH 2 Oxidative phosphorylation ATP Citric acid cycle Oxidative phosphorylation: electron transport and chemiosmosis

10. Fig. 9-10 CYTOSOLMITOCHONDRION NAD + NADH+ H + 2 1 3 Pyruvate Transport protein CO 2 Coenzyme A Acetyl CoA

12. Fig. 9-11 Pyruvate NAD + NADH + H + Acetyl CoA CO 2 CoA Citric acid cycle FADH 2 FAD CO 2 2 3 3 NAD + + 3 H + ADP +P i ATP NADH

15. Bitter Melon Selling my Body to Science 1.Consume a cup of liquid glucose. 2.Consume capsules that contain bitter melon OR placebo. 3.Lie still on a bed with a dome over my head and allow breath to be analyzed for an hour. QUESTIONS: Q1: On a molecular level, what is happening if bitter melon aids weight loss? Q2: On a molecular level, what is happening if bitter melon does not aid weight loss? Q3: Why were they interested in the air I exhaled? Q4: What do you think might happen to excess glucose in your body?

16. Glycolysis is a core metabolic pathway!

20. Campbell 8e Fig. 9.16

21. How does this compare to light reactions?

24. ETC: The main pieces Complex I: NADH dehydrogenase removes e - from NADH pumps 4 H + into inter- membrane space Complex II: succinate dehydrogenase moves e - from FADH 2 to Q Complex III: Cytochrome bc 1 transfers e - from Q to Cyt c pumps 2 H + into intermembrane space Complex IV: cytochrome c oxidase transfers e - from Cyt c to O 2 pumps 2H + into intermembrane space Q: ubiquinone, pumps 2 H + into intermembrane space Cyt c: cytochrome c e - shuttles

26. Disrupting cellular respiration: What happens if…..?

27. Cyanide – Binds to Cytochrome oxidase (Complex IV) – Immediate Effect? – Ultimate Effect?

28. Disrupting cellular respiration: What happens if…..? Rotenone – Binds to ubiquinone binding site of Complex I – Immediate Effect? – Ultimate Effect?

29. Disrupting cellular respiration: What happens if…..? Dinitrophenol – Protonophore (makes membrane completely permeable to H + ions) – Immediate Effect? – Ultimate Effect?

30. Anaerobic Respiration

31. (from wikipedia)

32. Homework Problem: Question: The Glucose Problem In the molecular process known as cellular respiration, how many ATP molecules can be made from the electron transport chain after one molecule of glucose is catabolized in glycolysis, pyruvate processing and the citric acid cycle. Assume that 1 ATP is produced when 3.5 protons pass through ATP synthase. **What pieces of information do I need to know to solve this problem?

33. Problem solving…

34. What would be the implications if a mitochondria was missing Complex II?

35. Problem solving… If Complex I was missing, what would net more ATP: Krebs Cycle OR ETC?

36. Problem solving… Can the ETC make energy (ATP) when we are not breathing? Why or why not?

37. Problem solving… Assume there was an unlimited supply of readily available NADH and an unlimited supply of readily available photons. If you had to choose one of the below two processes to make ATP, which would you choose (and why!)

38. For TUESDAY: 1.SN5 and HW4 (Version 2.0) 2.Honors Option Students – SIGN UP FOR THE FIELD TRIP (and show me the money)