1. How Cells Harvest Chemical Energy
Chapter 6
How Cells Harvest Chemical Energy 1

2. BREATHING VERSUS RESPIRATION
Alternation of inhalation and exhalation.
Exchange of gases in which organisms obtain oxygen from the air (or water) and release carbon dioxide.
Exchange occurs in lungs (or gills).
CELLULAR RESPIRATION:
Harvesting of energy from food molecules by cells.
Aerobic process (requires oxygen).
Occurs inside cells (cytoplasm and mitochondria).
“Respiration” comes from Latin word for breathing.
Breathing and cellular respiration are closely related, but not the same processes.

3. Breathing versus Cellular Respiration

4. CELLULAR RESPIRATION BANKS ATP REACTION:
C6H12O O > 6CO H2O + ENERGY
(Glucose) (Oxygen) (Carbon dioxide) (Water)
What happens to the energy in glucose or other food molecules?
Only about 40% of energy is turned into ATP
The rest is lost as metabolic heat.
One ATP molecule has about 1% of the chemical energy found in glucose.

5. ENERGY CONVERSIONS ARE INEFFICIENT Second Law of Thermodynamics
By Comparison Living Organisms Are Efficient

6. THREE MAJOR CATABOLIC PATHWAYS IN LIVING ORGANISMS
CATABOLISM:
Process of splitting larger molecules to smaller ones. Catabolic reactions are exergonic and release free energy.
THREE MAJOR CATABOLIC PATHWAYS IN LIVING ORGANISMS
A. Aerobic (Cellular) respiration
B. Anaerobic respiration
C. Fermentation

7. MAJOR CATABOLIC PATHWAYS A. Aerobic (Cellular) respiration:
Requires oxygen.
Most commonly used catabolic pathway.
Over 30 reactions. Used to extract energy from glucose molecules.
Final electron acceptor: Oxygen.
Most efficient: 40% of glucose energy is converted into ATP.
REACTION:
C6H12O O > 6CO H2O + ENERGY
Glucose Oxygen Carbon dioxide Water

8. THREE MAJOR CATABOLIC PATHWAYS B. Anaerobic respiration:
Does not require oxygen.
Used by bacteria that live in environments without oxygen.
Final electron acceptor: Inorganic molecule.
Very inefficient: Only 2% of glucose energy is converted into ATP.
Final products: Carbon dioxide, water, and other inorganic compounds.

9. THREE MAJOR CATABOLIC PATHWAYS C. Fermentation:
Does not require oxygen.
Used by yeast, bacteria, and other cells when oxygen is not available.
Final electron acceptor: Organic molecule.
Very inefficient: Only 2% of glucose energy is converted into ATP.
Products depend on type of fermentation:
Lactic acid fermentation: Used to make cheese and yogurt. Carried out by muscle cells if oxygen is low.
Alcoholic fermentation: Used to make alcoholic beverages. Produces alcohol and carbon dioxide.

10. II. Hydrogen carriers shuttle electrons in REDOX reactions
Oxidation:
Partial or complete loss of electrons or H atoms.
When a molecule is oxidized it loses energy.
Reduction:
Partial or complete gain of electrons or H atoms.
When a molecule is reduced it gains energy.
REDOX Reactions:
Reactions in which both oxidation and reduction occur.
Characteristic of many cell processes, including aerobic respiration and photosynthesis.

11. Cellullar Respiration is a Redox Process: Involves Both Oxidation and Reduction
Glucose is oxidized to carbon dioxide.
Oxygen is reduced to water.

12. III. Redox reactions in living organisms
Cellular Respiration: Macromolecules oxidized to release energy (686 kcal/mole) which is used to synthesize ATP
C6H12O O > 6CO H2O + ENERGY
Glucose Oxygen (oxidized) (reduced)
Photosynthesis: CO2 is reduced ; requires energy to drive the reaction forward
6CO H2O + ENERGY ---> C6H12O O2
Carbon Water (reduced) (oxidized)
Dioxide High Energy

13. IV. Hydrogen carriers shuttle electrons in redox reactions
1. Dehydrogenase: Removes hydrogen atoms (with their electrons) from organic molecules and transfers them to an electron carrier.
2. Electron Carrier Molecules:
NAD+: (Nicotinamide adenine dinucleotide) Coenzyme that accepts and transfers most H and the high energy electrons released by redox reactions
Reduction
NAD+ + 2H > NADH + H+
(2H+ & 2e-)
FADH2 (Flavin adenine dinucleotide): Secondary H carrier, related to NADH.

14. Dehydrogenase and Hydrogen Carriers Shuttle Electrons in Redox Reactions

15. IV. Electrons “fall” from Hydrogen Carriers to Oxygen in the Electron Transport Chain
NADH: (Nicotinamide andenine dinucleotide) : Delivers H and the high energy electrons released by redox reactions to electron carrier molecule of chain.
Electron transport chain: Proteins on inner mitochondrial membrane that accept H and use high energy electrons to produce ATP.

16. As Electrons “Fall” From Hydrogen Carriers
to Oxygen, Energy is Released

17. Two different means of ATP production:
1. Substrate-level phosphorylation:
Generates a small amount of ATP during cellular respiration.
Simple process, does not require membranes.
Phosphate group is directly transferred from an organic molecule to ADP to make ATP.
Occurs in first two stages of aerobic respiration:
Glycolysis
Kreb’s cycle

18. 2. Oxidative phosphorylation (Chemiosmosis):
Two different means of ATP production:
2. Oxidative phosphorylation (Chemiosmosis):
Generates most of ATP made during cellular respiration
Complex process, requires mitochondrial membranes.
Energy released from exergonic reactions of electron transport is used to pump H+ ions across membrane, creating a concentration gradient (potential energy).
Chemiosmosis: ATP is made by ATP synthase on mitochondrial membranes, as H+ flow down concentration gradient.
Occurs in last stage of aerobic respiration.
Requires the presence of OXYGEN

19. Two Mechanisms of ATP Synthesis:
Oxidative and Substrate Level Phosphorylation

20. V. Three Stages of Cellular Respiration
A. Glycolysis
B. Kreb’s Cycle
C. Electron Transport Chain & Chemiosmosis

21. Three Stages of Aerobic Respiration

22. A. Glycolysis: “Splitting sugar”
Occurs in the cytoplasm of the cell
Does not require oxygen
9 chemical reactions
Net result: Glucose molecule (6 carbons each) is split into two pyruvic acid molecules of 3 carbons each.
Yield per glucose molecule:
2 ATP ( Substrate-level phosphorylation)
2 NADH + 2 H+
(2 ATP are “invested” to get 4 ATP back)
Pyruvic acid diffuses into mitochondrial matrix where all subsequent reactions take place.

23. Glycolysis: “Splitting” of Glucose into Two Molecules of Pyruvic Acid

24. Conversion of Pyruvate to Acetyl CoA
Before entering the next stage, pyruvic acid (3C) must be converted to Acetyl CoA (2 C).
A carbon atom is lost as CO2.
Yield per glucose molecule: 2 NADH H+

26. Occurs in the matrix of the mitochondrion A cycle of 8 reactions
B. Kreb’s Cycle
Occurs in the matrix of the mitochondrion
A cycle of 8 reactions
Reaction 1: Acetyl CoA (2C) joins with 4C molecule (oxaloacetic acid) to produce citric acid (6C).
Reactions 2 & 3: Citric acid loses 2C atoms as CO2.
Reactions 4 & 5: REDOX reactions produce NADH and FADH2.
Reactions 6-8: Oxaloacetic acid is regenerated.

27. Kreb’s Cycle: Two Carbons In, Two Carbons Out

28. Details of Kreb’s Cycle

29. B. Kreb’s Cycle
Carbons are released as CO2
Yield per glucose molecule:
2 ATP (substrate-level phosphorylation)
6 NADH H+
2 FADH2

30. C. Electron Transport Chain & Chemiosmosis
Most ATP is produced at this stage
Occurs on inner mitochondrial membrane
Electrons from NADH and FADH2 are transferred to electron acceptors, which produces a proton gradient
Proton gradient used to drive synthesis of ATP.
Chemiosmosis: ATP synthase allows H+ to flow across inner mitochondrial membrane down concentration gradient, which produces ATP.
Ultimate acceptor of H+ and electrons is OXYGEN, producing water.

31. Electron Transport & Chemiosmosis: Generates
Most ATP Produced During Cellular Respiration

32. NOTE: The electron transport chain ONLY works when OXYGEN is available at the end of the chain to accept the electrons and H+ to form water.

33. C. Electron Transport Chain & Chemiosmosis
Yield of ATP through Chemiosmosis:
Each NADH produces 3 ATP
Each FAHD2 produces 2 ATP
2 NADH (Glycolysis) x 3 ATP = 6 ATP
2 NADH (Acetyl CoA) x 3 ATP = 6 ATP
6 NADH (Kreb’s cycle) x 3 ATP = 18 ATP
2 FADH2 (Kreb’s cycle) x 2 ATP = 4 ATP
________________
ATP
These ATPs are made by oxidative phosphorylation
or chemiosmosis.

34. VIII. Total Energy from cellular respiration
Substrate Oxidative
Process Phosphoryl e-Carrier Phosphoryl TOTAL
Glycolysis 2 ATP NADH ---> ATP ATP
Acetyl CoA NADH ---> 6 ATP ATP
Formation
Kreb’s ATP NADH ---> 18 ATP
2 FADH2 ---> 6 ATP ATP
__________
Total yield per glucose : ATP

36. Many poisons interrupt cellular respiration
Electron transport chain blockers:
Rotenone: Pesticide
Cyanide
Carbon monoxide
ATP synthase inhibitors
Oligomycin: Antifungal drug. Used on skin.
Uncouplers: Make mitochondrial membrane leaky to H+ ions. Abolishes H+ gradient, can’t make ATP through chemiosmosis.
Dinitrophenol (DNP): Used in 1940s as weight loss drug.

37. Effects of Various Poisons on the Electron Transport Chain

38. Yeasts normally use aerobic respiration to process food.
FERMENTATION OCCURS WHEN OXYGEN IS NOT AVAILABLE
Yeasts normally use aerobic respiration to process food.
If oxygen is not available, they use fermentation, which is less efficient.
Types of fermentation:
Alcoholic fermentation
Glucose ----> 2 pyruvate ----> 2 Ethanol CO2
Lactic acid fermentation
Glucose ----> 2 pyruvate ----> 2 Lactic acids

39. Fermentation Occurs When Oxygen is Unavailable

40. Alcoholic and Lactic Acid Fermentation: An Alternative to Aerobic Respiration

41. ORGANISMS CAN BE CLASSIFIED BASED ON THEIR OXYGEN REQUIREMENTS
Strict aerobes: Require oxygen for survival. All large organisms are aerobes.
Examples: Humans, dogs, insects.
Strict anaerobes: Grow only in the absence of oxygen. Are poisoned by oxygen.
Examples: Bacteria that live in soil and animal intestines.
Facultative anaerobes: Grow with/without oxygen. Grow better with oxygen.
Examples: Yeast and many bacteria.

42. All Food Molecules are Fed into The Catabolic
Pathway of Aerobic Respiration