1. Rodent Behavioral Learning and Memory Models
From Mechanisms of Memory by J. David Sweatt, Ph.D.

2. Variations of Fear Conditioning
TRAINING
CONTEXTUAL
TEST
CUED TEST
Cue-plus-contextual Fear Conditioning A.
Context Alone Conditioning
Context Discrimination
Context 1
Context 2 B. C.

3. Cued-Plus-Contextual Fear Conditioning
Training
Contextual

4. Contextual Fear Conditioning In Rats

5. Passive Avoidance Paradigm
Step-Through
Passive Avoidance
Step-Down
Passive Avoidance
Train
Train
Test:
measure time to step through
Test:
measure time to step down

6. Active Avoidance Paradigm
Shuttle Box
Active Avoidance
Test:
Can the animal avoid the shock?
Train

7. Passive Avoidance

8. Types of Rodent Mazes Lashley Maze 4 Arm Radial (Plus) Maze “Y” Maze
Start
Finish
Lashley Maze
4 Arm Radial (Plus) Maze
“Y” Maze
“T” Maze
Triangle Maze
8 Arm Radial Maze

9. The Morris Water Maze
Camera
Distal Visual Cues
Platform

10. Experimental Results: Morris Water Maze
Hidden Platform
Probe Test
0.0
10.0
20.0
30.0
40.0
50.0
60.0 1 2 3 4 5 6 7 8 9 10
Blocks of 4 trials
Escape Latency (sec)
0.0
1.0
2.0
3.0
4.0
5.0
129/SvImJ
Platform Crossings
10.0
20.0
30.0
40.0
50.0
60.0 1
Quadrant Search Time (%)
Opposite
Right
Trained
Left
Visible Platform
0.0
10.0
20.0
30.0
40.0
50.0
60.0 1 2 3 4 5 6
Blocks of 4 trials
Escape Latency (sec)

11. Path Tracking in the Probe Test
Vehicle
30 mg/kg SL327
Subject #66
Path Length = cm
Subject #57
Path Length = cm

12. The Barnes Maze Escape Chamber Spatial Cues Search strategy A
Search strategy B

13. Conditioned Taste Aversion
DAY 1 B
1.0
1st Exposure to Food Item
0.8
0.6
grams blueberry bar
0.4
Injection of LiCl or NaCl
0.2
DAY 2
0.0
LiCl
NaCl
Grams Ingested on 2nd Exposure
(LiCl treated group compared to NaCl treated control)
2nd Exposure to Food Item

14. (1st exposure compared to second exposure)
Neophobia A B
DAY 1
1st Exposure to Food Item
0.0
0.2
0.4
0.6
0.8
1st intake
2nd intake
grams blueberry bar
DAY 2
2nd Exposure to Food Item
Grams Ingested
(1st exposure compared to second exposure)

15. Four Types of Experiments
Hypothesis: A→B→C
Experiment
Prediction
• Determine
• None (A makes C happen)
• Block
• Blocking B should block A causing C
• Mimic
• Activating B should cause C
• Measure
• A makes B happen

16. Contextual conditioning and CRE-mediated Gene Expression in Mice transgenic for CRE-LAC Z

17. Hippocampal Protein Kinase Activation
in Fear Conditioning

18. Open Field Behavior A C B Center/Total Distance Total Distance
Vertical Activity

19. Rotating Rod Behavior

20. Acoustic Startle and Pre-Pulse Inhibition Tests

21. Nociception Behavior Hot Plate Test Hind Paw Lick Latency (s) 10 2010 20
+/+ n=26
-/- n=20
Hind Paw Lick Latency (s)

22. Elicits Eyeblink Response Elicits Eyeblink Response
Eye-Blink Conditioning in Rabbits
Corneal Air Puff
Elicits Eyeblink Response
Corneal Air Puff
Given with Tone
Tone Given Alone
Elicits Eyeblink Response

23. “of Mice and Rats”