1. Review Lab Exercises 8-11

2. Exercise 8 zWhat is the purpose of this exercise? zWhat is a capsule? zWhat is the capsule made of? zWhat is the importance of the capsule? zDescribe the staining procedure for staining the capsule zWhat bacteria we use for the capsule stain procedure?

3. Exercise 8 cont. zWhat is a flagella? zWhat is taxis? zWhy do we stain the flagella? zWhat bacteria we observed the flagella? zWhat is an endospore? zWhy do bacteria produce spores? zDescribe the composition of the endospore

4. Exercise 8 cont. zWhen do endospores dissolve? zDescribe the procedure to stain endospores zWhat bacteria we use for the endospore stain procedure? zWhat is mycolic acid? zWhat antibiotics are used against TB? zDescribe the procedure for acid fast stain zWhat bacterias we use for this staining procedure?

5. Exercise 9 zWhat is the purpose of the exercise? zExplain the major steps involved in obtaining pure cultures from a mixed population zExplain the difference between selective and differential media zDescribe the appearance of the following bacteria in EMB yE. coli, Enterobacter, Klebsiella, Proteus, Salmonella, Shigella zExplain the color changes on EMB

6. Exercise 9 cont. zDescribe the growth of the following bacterias on TSA yM. luteus, S. epiderdimis, E. coli, E. aerogenes zDescribe the growth of the following bacterias on EMB yM. luteus, S. epiderdimis, E. coli, E. aerogenes zDescribe the growth of the following bacterias on CNA yM. luteus, S. epiderdimis, E. coli, E. aerogenes

7. Exercise 10 zWhat is the purpose of this exercise? zExplain the composition of the blood agar? zDifferentiate between beta, alpha, and gamma hemolysis on blood agar zDescribe the composition and use of Mannitol Salt Agar (MSA) zExplain the catalase test

8. Exercise 10 cont. zExplain the coagulase test zExplain the bacitracin or Taxo A disc test zDescribe the composition and use of the Bile Esculin Agar zExplain the 6.5% NaCl testing zExplain the Optochin or Taxo P disc test

9. Exercise 10 cont. zDescribe the growth on blood Agar of: yS. aureus, S. epidermidis, E. faecalis, S. bovis zDescribe the growth on MSA of: ySt. aureus, S. epidermidis, E. coli zDescribe the coagulase test on S. aureus and S. epidermidis zDescribe the growth of E. faecalis and S. bovis on Bile Esculin Agar

10. Exercise 10 cont. zDescribe the growth of S. bovis and E. faecalis on 6.5% NaCl zDescribe the growth of S. pyogenes with Taxo A zDescribe the growth of S. pneumoniae with Taxo P

11. Exercise 11 zWhat is the purpose of the exercise? zHow do you differentiate Staphylococcus and Streptococcus. zHow do you know if you have S. pyogenes in your throat?