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Quality Control of Culture media Dr Mohammad Rahbar Iranian Reference Health Laboratory.

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Presentation on theme: "Quality Control of Culture media Dr Mohammad Rahbar Iranian Reference Health Laboratory."— Presentation transcript:

1 Quality Control of Culture media Dr Mohammad Rahbar Iranian Reference Health Laboratory

2 Anaerobic sheep blood and laked blood agar Incubation Atmosphere Anaerobic Length, and Temperature h, 35 °C Control Organisms(ATCC ) Expected Reults B. fragilis (25285) :Growth C. perfringens (13124) : Growth beta hemolysis F. nucleatum (25586) : Growth P. anaerobius (27337): Growth P. melaninogenica (25845) : Growth

3 Blood agar—nonselective sheep blood agar media Incubation Atmosphere, Aerobic or CO2, Length, and Temperature; h, 35 °C Control Organisms (ATCC® No.) Expected Results S. pyogenes (19615) : Growth beta hemolysis S. pneumoniae (6305) : Growth alpha hemolysis S. aureus (25923) : growth E. coli (25922) : Growth

4 Blood agar-CAMP test (trypticase soy agar [TSA] with sheep blood only) Incubation Atmosphere :Anaerobi Length, and Temperature :24-48 h, 35 °C Control Organisms ATCC S. aureus (33862) or (25923):Growth S. agalactiae (12386) :Positive reaction (Arrowhead area of clearing) S. pyogenes (19615) :Negative reaction (No arrowhead formation)

5 Blood agar—Selective sheep blood agar media (Columbia [CNA] agar, Incubation Atmosphere : CO2° Length, and Temperature :24-48 h, 35 °C Control Organisms : Expected Results S. pyogenes (19615) : Growth mbetahemolyric S. pneumoniae (6305) : Growth alpha hemolytic S. aureus (25923) Growth P. mirabilis (12453) : Growth

6 Blood agar—Selective sheep blood agar media (Columbia [CNA] agar, Incubation Atmosphere : CO2° Length, and Temperature :24-48 h, 35 °C Control Organisms : Expected Results S. pyogenes (19615) : Growth mbetahemolyric S. aureus (25923) Growth P. mirabilis (12453): Inhibition (partial)

7 Blood culture media. (TSI and BHI) Incubation Atmosphere : Anaerobic and aerobic Length, and Temperature : 5 days, 35 °C Control Organisms : Expected Results B. fragilis (25285): Growth S. pneumoniae (6305) : Growth

8 Campylobacter agar (user quality control required ) Incubation Atmosphere :Reduced O2 with CO2, Length, and Temperature : 48 h, 42 °C Control Organisms : Expected Results C. jejuni (33291) : Growth E. coli (25922): Inhibition (partial)

9 Chocolate agar Incubation Atmosphere : CO2 Length, and Temperature :24-48 h, 35 °C Control Organisms ATCC N. gonorrhoeae (43069) : Growth H. influenzae (10211) : gROWTH

10 Cefsulodin irgasan novobiocin (CIN) agar Incubation Atmosphere : Aerobic Length, and Temperature : h, 25 oC Control Organisms ATCC: Y. enterocolitica (9610) :Growth; deep red center transparent border (bull’seye) E. coli (25922) :Inhibition (partial to complete) P. aeruginosa (27853) Inhibition (partial to complete) E. faecalis (29212) Inhibition (partial to complete)

11 Buffered charcoal yeast extract (BCYE) (CYE/BCYE) agar Incubation Atmosphere,:Aerobic Length, and Temperature :48-72 h, 35 °C Control Organisms L. pneumophila (33152) :Growth; yellow-green fluorescence under long-wave UV light L. bozemanii (33217) :Growth; blue-white fluorescence L. micdadei (33204 :Growth

12 Enrichment broths for enterics (gram-negative [GN] broth, selenite broths) Incubation Atmosphere,:Aerobic Length, and Temperature :18-24h, 35 °C Control Organisms S. typhimurium (14028) : Growth on subculture S. sonnei (9290) : Growth on subculture (may be inhibited by selenite media) E. coli (25922): Inhibition (partial to complete) on subculture Growth on subculture from GN broth

13 Eosin methylene blue media (Levine EMB agar; EMB agar, modified) Incubation Atmosphere,: Aerobic Length, and Temperature : h, 35 °C Control Organisms: S. typhimurium (14028) : Growth, colorless to amber colonies E. coli (25922): Growth, blue-black colonies with green metallic sheen E. faecalis (29212): Inhibition (partial)

14 Hektoen enteric (HEK) agar Incubation Atmosphere, :Aerobic Length, and Temperature :18-24 h, 35 °C Control Organism: S. typhimurium (14028): Growth, colonies blue to green-blue with black centers S. flexneri (12022) :Growth, colonies green to blue- green E. faecalis (29212):Inhibition (partial); colonies yellow E. coli (25922) :Inhibition (partial to complete); colonies yellow to salmon colored

15 MacConkey agar Incubation Atmosphere, :Aerobic Length, and Temperature :18-24 h, 35 °C Control Organism E. coli (25922) : Growth, pink colonies P. mirabilis (12453) : Growth, colorless colonies, partial inhibition of swarming S. typhimurium (14028): Growth, colorless colonies E. faecalis (29212) : Inhibition (partial)

16 Mannitol salt agar Incubation Atmosphere, :Aerobic Length, and Temperature :24-48h, 35 °C Control Organism; S. aureus (25923) : Growth, colonies have yellow zones at 48 h S. epidermidis (12228): Growth, colonies have red zones at 48 h P. mirabilis (12453) : Inhibition (partial)

17 Salmonella-Shigella (SS) agar Incubation Atmosphere, :Aerobic Length, and Temperature :24 h, 35 °C Control Organism S. typhimurium (14028) : Growth, colonies colorless with or without black centers S. flexneri (12022) : Growth, colorless colonies E. faecalis (29212) : Inhibition (complete) E. coli (25922) : Inhibition (partial to complete; colonies pink to rose-red with precipitate)

18 Selective media for pathogenic Neisseria spp.(TM) Incubation Atmosphere, :Co2 Length, and Temperature :24 -48h, 35 °C Control Organism: N. gonorrhoeae (43069) :Growth N. meningitidis (13090) :Growth P. mirabilis (43071) :Inhibition (partial) use only for media containing trimethoprim E. coli (25922) :Inhibition (partial) N. sicca (9913) :Inhibition (partial) C. albicans (60193) :Inhibition (partial) S. epidermidis (12228) :Inhibition (partial)

19 Selective media for enterococci, with azide Incubation Atmosphere, :Aerobic Length, and Temperature :24 -48h, 35 °C Control Organism: E. faecalis (29212) :Growth, blackening around colonies S. pyogenes (19615):Inhibition (partial to complete) E. coli (25922):Inhibition (partial) - Colorless colonies on bile esculin agar

20 Selective media for enterococci, without azide Incubation Atmosphere, :Aerobic Length, and Temperature :24 -48h, 35 °C Control Organism: E. faecalis (29212) :Growth, blackening around colonies S. pyogenes (19615):Inhibition (partial to complete)

21 Thioglycolate broth, with or without indicator Incubation Atmosphere, :Aerobic, 48 h (tightened, cap Length, and Temperature : 48h, 35 °C Control Organism: B. fragilis (25285) : Growth S. aureus (25923): Growth

22 Thioglycolate broth, enriched with vitamin K and hemin Incubation Atmosphere, :Aerobic, 48 h (tightened, cap Length, and Temperature : 48h, 35 °C Control Organism: P. anaerobius (27337) : Growth B. vulgatus (8482): Growth C. perfringens (13124) : Growth

23 Tubed media (brain heart infusion and tryptic soy broth ) Incubation Atmosphere, :Aerobic Length, and Temperature :18-24, 35 °C Control Organism: E. coli (25922) : Growth S. aureus (25923) : Growth

24 Xylose lysine desoxycholate (XLD) agar Incubation Atmosphere, :Aerobic Length, and Temperature : 24, 35 °C Control Organism: S. typhimurium (14028) :Growth - colonies red with black centers S. flexneri (12022) :Growth - colonies red E. faecalis (29212) :Inhibition partial E. coli (25922) :Inhibition (partial to complete; colonies yellow to yellow-red)


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