1. Introduction to Light and Electron Microscopy NEU259 2012 Gina Sosinsky, Ph. D. Mark Ellisman, Ph. D.

2. Class Info Tues-Thurs 10:00-11:00 am lecture Tues-Thurs 10:00-11:00 am lecture NCMIR conference room NCMIR conference room Thursday 11-1 pm demonstrations Thursday 11-1 pm demonstrations Usually at NCMIR but may be elsewhere on campus Usually at NCMIR but may be elsewhere on campus Class limited to ~20 students total Class limited to ~20 students total Preference is given to enrolled students Preference is given to enrolled students Syllabus and lectures will be posted on: Syllabus and lectures will be posted on: http://ncmir.ucsd.edu/training/ucsd_courses/index.shtm http://ncmir.ucsd.edu/training/ucsd_courses/index.shtm http://ncmir.ucsd.edu/training/ucsd_courses/index.shtm http://cryoem.ucsd.edu/courses.shtm. http://cryoem.ucsd.edu/courses.shtm. http://cryoem.ucsd.edu/courses.shtm

4. Coursework Midterm (in class) Midterm (in class) Final (take home) Final (take home) Student presentations (last class, extra credit) Student presentations (last class, extra credit) My thesis project and how microscopy will be used My thesis project and how microscopy will be used Any readings will be made available in class, via email or from Jennifer Santos in our admin office. Any readings will be made available in class, via email or from Jennifer Santos in our admin office. Grades: Pass/Fail Grades: Pass/Fail No letter grade will be given for this class; we can file documentation to the department indicating the grade that would have been given. No letter grade will be given for this class; we can file documentation to the department indicating the grade that would have been given. Check with the department if you need a letter grade before taking the class to see if they will accept a letter from the instructors Check with the department if you need a letter grade before taking the class to see if they will accept a letter from the instructors

5. Workshop in Light and Electron Microscopy (Lab Class) NEU260 NEU260 Practical course in light and electron microscopy Practical course in light and electron microscopy Hands on training in confocal, multiphoton, specimen preparation, TEM, electron tomography, cryo-electron microscopy Hands on training in confocal, multiphoton, specimen preparation, TEM, electron tomography, cryo-electron microscopy http://ncmir.ucsd.edu/Training/UCSDCourses/current.htm http://ncmir.ucsd.edu/Training/UCSDCourses/current.htm http://ncmir.ucsd.edu/Training/UCSDCourses/current.htm Tues-Thurs 1-3 pm* Tues-Thurs 1-3 pm* May require extra time during student projects & training. May require extra time during student projects & training. NEU259 is pre- or co-requisite for practical course NEU259 is pre- or co-requisite for practical course Offered through Interfaces Training Program Offered through Interfaces Training ProgramInterfaces Training ProgramInterfaces Training Program

6. Interfaces Training Program Interfaces Training Program The Interfaces Training Program is an interdisciplinary graduate training program at the interfaces between the Biological, Medical, Physical and Engineering Sciences. Courses are all lab courses. UCSD was one of ten universities selected through the Howard Hughes Medical Institute (HHMI) to initiate this new program. There is now a NIH NIBIB training grant for the program and UCSD support for the lab courses.

7. Courses Logo designed by Interfaces student(s).

8. Program Goals Program Goals 1) Focused Collaboration across nine graduate degree programs at UCSD to train a new generation of cross-disciplinary scientist. 2) State-of-the-art Interdisciplinary Training through a new technology-centered hands-on graduate laboratory course curriculum. 3) Novel Emphasis on Research aimed at integrative and quantitative analysis across multiple scales of biological organization from molecule to organism in health and disease.

9. Participating Programs / Departments Department of Bioengineering Division of Biological Sciences Bioinformatics Graduate Program Biomedical Sciences Graduate Program Department of Chemistry and Biochemistry Department of Mathematics Molecular Pathology Ph.D. Program Neurosciences Graduate Program Department of Physics Department of Radiology

10. Participating Faculty 25 outstanding faculty members Appointed representatives from all participating degree programs plus the Radiology Department Core leaders and Co-instructors for six new graduate laboratory courses Includes three investigators of the Howard Hughes Medical Institute Two faculty with appointments at the neighboring Salk and Burnham Institutes

11. Application and Admission Students interested in participating must: 1) First, be admitted through one of the participating doctoral programs and/or departments: Bioengineering, Bioinformatics, Biology, Biomedical Sciences, Chemistry and Biochemistry, Mathematics, Neuroscience, Molecular Pathology, and Physics. Send e-mail to Irene Jacobo ( 2) Then, join a Student Affiliate by completing a Student Affiliate Application. Send e-mail to Irene Jacobo ( ihjacobo@ucsd.edu)

12. Student Affiliates Student Affiliates will participate in: Interfaces Course Enrollment Funding through Teaching Assistantships Multi-Scale Biology Specialization Special Events Funding through Formal Traineeships

13. Curriculum and Training Curriculum Includes Six hands-on graduate laboratory courses taught by interdisciplinary teams of faculty members Labs will introduce students to state-of-the-art techniques studying living systems across physical scales from molecules to the whole organism multi-scale techniques for measuring, imaging, manipulating and analyzing living systems Labs are designed to train students for modern research opportunities in integrative multi-scale biomedical science