1. Bioconversion of biomass to ethanol-an overview Renata Bura November 25 th, 2008

2. Overview What is bioconversion? Why bioconversion? Sugar cane to ethanol Corn to ethanol Biomass composition Bioconversion to ethanol process –Pretreatment –Hydrolysis –Fermentation Pros and cons of bioethanol

3. What is bioconversion? General: a process which uses biological agents (microorganisms or protein) to transform a feedstock into desirable products. Bioethanol A chemical/biochemical process by which lignocellulosic materials are converted to ethanol and other co- products.

4. Bioconversion Biomass Pretreatment Hydrolysis Fermentation Distillation Ethanol

5. Why bioethanol?

6. Ethanol (CH 3 CH 2 OH) Ethyl alcohol, grain alcohol –Clear, colorless liquid Ethanol made from cellulosic biomass instead of starch crops-bioethanol Advantages of bioethanol –Domestic renewable fuel sources –Reduces reliance on foreign oil –Cleaner fuel sources –Easily produced and stored –Increases fuel octane number for little cost

7. Energy content Gasoline Ethanol Biodiesel 100% 67% 86%

8. World ethanol production

9. Sugar cane, sugar cane bagasse Sugar cane bagasse Processing Ethanol

10. Sugar cane bioethanol Brazil produces 3,96 billion gallons of ethanol from sugar cane Production cost $0.87/gallon, the lowest in the world Fossil fuel energy used to make the fuel (input) compared with energy in the fuel (output) 1:8 Green house emission during production and use 56% less compared with gasoline

11. Corn plant Corn kernel (without the fibre)-starch alcohol Corn fibre-lignocellulosic alcohol Corn stover-lignocellulosic alcohol

13. Corn to ethanol US produces 4,86 billion gallons of ethanol from corn (2006) Production cost ~ $1.09/gallon Fossil fuel energy used to make the fuel (input) compared with energy in the fuel (output) 1:1.3 or negatives values Greenhouse gas emission during production and use 22% less compared with gasoline

14. “We can get fuel from apples, weeds, sawdust, almost anything….. And it remains for someone to find how this fuel can be produced commercially- better fuel at a better price than we now know.” Henry Ford Henry Ford

15. Why bioconversion? Energy –An alternative source of energy for the transportation sector produced locally Air pollution –Reduction in greenhouse gas emission Waste elimination –Elimination of problems with field burning/incineration, stockpiling, etc. Socio/economical benefits –Creation of new jobs, rural development

16. Possible lignocellulosic feedstocks Agricultural residues (corn stover, corn fibre, wheat straw, rice straw) Wood residues Paper waste Municipal solids waste

17. Biomass composition

18. Cellulose

19. Hemicellulose

20. Lignin

21. Bioconversion of biomass to ethanol (pretreatment) Biomass Pretreatment Liquid phase Solid phase Cellulose Sugars Ethanol Fermentation Ethanol Sugars Fermentation Hydrolysis Lignin Recovery

22. Pretreatment-”disruption” Helps in separation of main biomass components (cellulose, hemicellulose and lignin) Increase available surface area Reduce particle size

23. Steam explosion Treatment of biomass with high-pressure steam for a short period of time followed by sudden decompression Acid (H 2 SO 4, SO 2 ) impregnation of biomass increases SE efficiency Typical conditions: –Temperature: 170-250 o C, 338-482 F –Time: 10sec-10min

24. Steam gun Karin Fill valve Steam valve Blow valve Receiving vessel Receiving vessel Pretreated corn stover

25. Bioconversion of biomass to ethanol (hydrolysis) Biomass Pretreatment Liquid phase Solid phase Cellulose Sugars Ethanol Fermentation Ethanol Sugars Fermentation Hydrolysis Lignin Recovery

26. What are cellulases? Produced by many strains of bacteria and fungi Catalyzes the depolymerization of cellulose chains –Endoglucanases –Exoglucanases –β-glucosidases

27. Cellulases Endoglucanases (EG) cutting the cellulose chains randomly Cellobiohydrolyses (CBH) cutting cellobiose units of the ends of the cellulose chains Binding domainCatalytic domain

28. Bioconversion of biomass to ethanol (fermentation) Biomass Pretreatment Liquid phase Solid phase Cellulose Sugars Ethanol Fermentation Ethanol Sugars Fermentation Hydrolysis Lignin Recovery

29. Fermentation Defined as: Cellular metabolism under anaerobic conditions (absence of oxygen) for the production of energy and metabolic intermediates Many organisms can “ferment” (i.e., grow anaerobically) Not all produce ethanol as an end-product of fermentation

30. Strain selection Choice of microorganism for ethanol production has traditionally been a Yeast Yeast: –Single cell microorganism –Fungi –Facultative anaerobe Most common industrial fermenter is Saccharomyces cerevisiae (baker’s or brewer’s yeast) Why?

31. Fermentation Conversion factor 0.51 1g/L of glucose: 0.51g/L ethanol (maximum)

32. Biofuels-comparison Production (billion gallons) Production cost ($) Energy balance GHE reduction (%) Corn4.861.091.322 Cane3.960.878.055-90 LignocelluloseNA 2-3691 Biodiesel0.50NA2.568

33. Alternative options??…….

34. Flexible-Fuel Vehicles (FFV) Use E85 (85% ethanol and 15% gasoline) Cost of FFV is similar to traditional gasoline vehicle 1gallon of E85 provides the same energy as 0.72 gallons of gasoline (lower E content) Special materials required for fuel lines, hoses, valves, gaskets, fuel tank (corrosive ethanol) Washington state more than 35, 000 of FFVs (U.S. over 4 million FFVs) –Ford Focus, Chrysler Sebring, Dodge Stratus, Dodge Caravan, Chevrolet Avalanche

35. Summary What is bioconversion? Why bioconversion? Sugar cane to ethanol Corn to ethanol Biomass composition Bioconversion to ethanol process –Pretreatment –Hydrolysis –Fermentation Pros and cons FFV

37. References www.ento.vt.edu/~salom/SPBbiology/blustain.html www.pherotech.com/new_products.html www.dnr.wa.gov/../issues/2002issues.html www.laco.ufpe.br/prh28/ www.bio-pro.de/en/region.rhein/magazin/01440 www.maize.agron.iastate.edu/corngrows.html www.nrel.gov www.energy.iastae.edu www.usda.gov/wps/portal/usdahome www.ethanol-gec.org/clean/cf13.htm www.eere.energy.gov/biomass/