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University of Ghent, 2000 Alfonso Valencia %identity with a protein of known structure 0%0% 100 % 30 % Homology modeling Threading Ab initio 3D Structure.

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Presentation on theme: "University of Ghent, 2000 Alfonso Valencia %identity with a protein of known structure 0%0% 100 % 30 % Homology modeling Threading Ab initio 3D Structure."— Presentation transcript:

1 University of Ghent, 2000 Alfonso Valencia %identity with a protein of known structure 0%0% 100 % 30 % Homology modeling Threading Ab initio 3D Structure prediction

2 University of Ghent, 2000 Alfonso Valencia Protein fold recognition by prediction-based threading "TOPITS". Rost, Schneider, Sander (1997) J. Mol. Biol. 270, 471-480 Secondary structure prediction Prediction- based threading

3 University of Ghent, 2000 Alfonso Valencia Pazos, Rost, Valencia 1999 Threadlize Interactive inspection and manipulation of threading models http://gredos.cnb.uam.es

4 University of Ghent, 2000 Alfonso Valencia

5 University of Ghent, 2000 Alfonso Valencia Plotcor r http://gredos.cnb.uam.es Pazos, Olmea & Valencia, 1997

6 University of Ghent, 2000 Alfonso Valencia Sequence features related to structure and function

7 University of Ghent, 2000 Alfonso Valencia CASP2 (1997) Fold Recognition (Threading)

8 University of Ghent, 2000 Alfonso Valencia CASP3 (1999). Threading.

9 University of Ghent, 2000 Alfonso Valencia CASP4 (2000): our predicitons by D. Devos

10 University of Ghent, 2000 Alfonso Valencia - Threading prog. Score - Cons. Res. Closeness. - Tree-det " - Corr. Mut " - Apolar res. " - Fold compactness - Fold predicted by diff. Threaders. Threadin g model

11 University of Ghent, 2000 Alfonso Valencia Threading server. Proc. Control: Flowchart CGI Start Parameter Checking Valid email address 'Threadable' seq. Valid seq. format Fork & Detach Threaders Launching Threader 2 PredictProtein 3D-PSSM SAM-T98 HMM ERROR!!! Aborting... Sleep Check email Match & SaveDiscard Follow, TimeOut or Finish Threader 2 PredictProtein 3D-PSSM SAM-T98 HMM Run Threadlize PostProcessing Send Results or Full Working tree by J.M. Fernández

12 University of Ghent, 2000 Alfonso Valencia Threading server methods by F. Pazos

13 University of Ghent, 2000 Alfonso Valencia by F. Pazos CAFASP2 (2000) Servers for Automatic Structure Predictions

14 University of Ghent, 2000 Alfonso Valencia http://gredos.cnb.uam.es


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