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Protein structure prediction.. Protein folds. Fold definition: two folds are similar if they have a similar arrangement of SSEs (architecture) and connectivity.

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Presentation on theme: "Protein structure prediction.. Protein folds. Fold definition: two folds are similar if they have a similar arrangement of SSEs (architecture) and connectivity."— Presentation transcript:

1 Protein structure prediction.

2 Protein folds. Fold definition: two folds are similar if they have a similar arrangement of SSEs (architecture) and connectivity (topology). Sometimes a few SSEs may be missing. Fold classification: structural similarity between folds is searched using structure- structure comparison algorithms.

3 Protein structure prediction flowchart Protein sequence Database similarity search Does sequence align with a protein of known structure? Protein family analysis Relationship to known structure? Three-dimensional comparative modeling Predicted three- dimensional structural model Structural analysis Is there a predicted structure? Three- dimensional structural analysis in laboratory No Yes NoYes No From D.W.Mount

4 Protein structure prediction. Prediction of three-dimensional structure of a protein from its sequence. Different approaches: -Homology modeling (query protein has a very close homolog in the structure database). -Fold recognition (query protein can be mapped to template protein with the existing fold). -Ab initio prediction (query protein has a new fold).

5 Homology modeling. Aims to produce protein models with accuracy close to experimental and is used for: -Protein structure prediction -Drug design -Prediction of functionally important sites (active or binding sites)

6 Steps of homology modeling. 1.Template recognition & initial alignment. 2.Backbone generation. 3.Loop modeling. 4.Side-chain modeling. 5.Model optimization.

7 1. Template recognition. Recognition of similarity between the target and template. Target – protein with unknown structure. Template – protein with known structure. Main difficulty – deciding which template to pick, multiple choices/template structures. Template structure can be found by searching for structures in PDB using pairwise sequence alignment methods.

8 Two zones of protein structure prediction Homology modeling zone Fold recognition zone Alignment length Sequence identity

9 2. Backbone generation. If alignment between target and template is ready, copy the backbone coordinates of those template residues that are aligned. If two aligned residues are the same, copy their side chain coordinates as well.

10 3. Insertions and deletions. insertion AHYATPTTT AH---TPSS deletion Occur mostly between secondary structures, in the loop regions. Loop conformations – difficult to predict. Approaches to loop modeling: -Knowledge-based: search the PDB for loops with known structures -Energy-based: an energy function is used to evaluate the quality of a loop. Energy minimization or Monte Carlo.

11 4. Side chain modeling. Side chain conformations – rotamers. In similar proteins - side chains have similar conformations. If % identity is high - side chain conformations can be copied from template to target. If % identity is not very high - modeling of side chains using libraries of rotamers and different rotamers are scored with energy functions. Problem: side chain configurations depend on backbone conformation which is predicted, not real E1E1 E2E2 E3E3 E = min(E1, E2, E3)

12 5. Model optimization. Energy optimization of entire structure. Since conformation of backbone depends on conformations of side chains and vice versa - iteration approach: Predict rotamersShift in backbone

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14 Classwork: Homology modeling. -Go to NCBI Entrez, search for gi Do Blast search against PDB -Repeat the same for gi Compare the results

15 Fold recognition. Unsolved problem: direct prediction of protein structure from the physico-chemical principles. Solved problem: to recognize, which of known folds are similar to the fold of unknown protein. Fold recognition is based on observations/assumptions: -The overall number of different protein folds is limited ( folds) -The native protein structure is in its ground state (minimum energy)

16 Fold recognition. Goal: to find protein with known structure which best matches a given sequence. Since similarity between target and the closest template is not high, pairwise sequence alignment methods fail. Solution: threading – sequence-structure alignment method.

17 Threading – method for structure prediction. Sequence-structure alignment, target sequence is compared to all structural templates from the database. Requires: -Alignment method (dynamic programming, Monte Carlo,…) -Scoring function, which yields relative score for each alternative alignment

18 Target sequence Structural templates Score1Score2Score3 Protein structure prediction: target sequence is compared to structures using sequence- structure alignment Concept of threading: D. Jones et al, 1993

19 Target sequence Structural templates Score1 Score2Score3 Structural model of target Score3>Score2>Score1 Protein structure prediction: target sequence is compared to structures using sequence- structure alignment

20 Scoring function for threading. Contact-based scoring function depends on amino acid types of two residues and distance between them. Sequence-sequence alignment scoring function does not depend on the distance between two residues. If distance between two non- adjacent residues in the template is less than 8 Å, these residues make a contact.

21 Scoring function for threading. Ala Ile Tyr Trp “w” is calculated from the frequency of amino acid contacts in PDB; a i – amino acid type of target sequence aligned with the position “i” of the template; N- number of contacts

22 Classwork: calculate the score for target sequence “ATPIIGGLPY” aligned to template structure which is defined by the contact matrix *** 2 3* 4* 5** 6* 7* 8* 9 ** ATPYIGL A T P Y I G 0.2 L0.3

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24 Evaluation of quality of structural model Correct bond length and bond angles Correct placement of functionally important sites Prediction of global topology, not partial alignment (minimum number of gaps) >> 3.8 Angstroms

25 Success and limitations of structure prediction Success: Accuracy scores almost doubled from CASP1 to CASP6, might be because of database size Models of small targets are very accurate Adapted from Kryshtafovych et al 2005 Limitations: Models of large and remotely related proteins are not very accurate Domain boundaries are difficult to define Models often do not provide details for functional annotation

26 GenThreader 1.Predicts secondary structures for target sequence. 2.Makes sequence profiles (PSSMs) for each template sequence. 3.Uses threading scoring function to find the best matching profile.

27 Protein-protein interactions.

28 Common properties of protein-protein interactions. Majority of protein complexes have a buried surface area ~1600±400 Ǻ^2 (“standard size” patch). Complexes of “standard size” do not involve large conformational changes while large complexes do. Protein recognition site consists of a completely buried core and a partially accessible rim. Trp and Tyr are abundant in the core, but Ser and Thr, Lys and Glu are particularly disfavored. Top molecule Bottom molecule rim core

29 Different types of protein-protein interactions. Permanent and transient. External are between different chains; internal are within the same chain. Homo- and hetero-oligomers depending on the similarity between interacting subunits. Interface type can be predicted from amino acid composition (Ofran and Rost 2003).

30 Experimental methods

31 Verification of experimental protein-protein interactions. Protein localization method. Expression profile reliability method. Paralogous verification method.

32 Protein localization method. Sprinzak, Sattath, Margalit, J Mol Biol, 2003 A – A3: Y2H B: physical methods C: genetics E: immunological True positives: -Proteins which are localized in the same cellular compartment -Proteins with a common cellular role

33 Deane, C. M. (2002) Mol. Cell. Proteomics 1: Expression profile reliability method.

34 Deane, C. M. (2002) Mol. Cell. Proteomics 1: Paralogous verification method. PVM method is based on observation that if two proteins interact, their paralogs would interact. Calculates the number of interactions between two families of paralogous proteins.

35 Interaction databases Experiment (E) Structure detail (S) Predicted –Physical (P) –Functional (F) Curated (C) Homology modeling (H) *IMEx consortium

36 Protein interaction databases Protein-protein interaction databases Domain-domain interaction databases

37 DIP database Documents protein- protein interactions from experiment –Y2H, protein microarrays, TAP/MS, PDB 55,733 interactions between 19,053 proteins from 110 organisms. Organisms# proteins# interactions Fruit fly705220,988 H. pylori Human E. coli C. elegans Yeast492118,225 Others985401

38 DIP database Duan et al., Mol Cell Proteomics, 2002 Assess quality –Via proteins: PVM, EPR –Via domains: DPV Search by BLAST or identifiers / text

39 BIND database Records experimental interaction data 83,517 protein-protein interactions 204,468 total interactions Includes small molecules, NAs, complexes Alfarano et al., Nucleic Acids Res, 2005

40 Classwork. Go to DIP webpage (http://dip.doe- mbi.ucla.edu)http://dip.doe- mbi.ucla.edu Retrieve all interactions for cytochrome C, tubulin, RNA-polymerase from yeast How many of them are confirmed by several experimental methods?

41 Protein interaction databases Protein-protein interaction databases Domain-domain interaction databases

42 InterDom database Predicts domain interactions (~30000) from PPIs Data sources: –Domain fusions –PPI from DIP –Protein complexes –Literature Scores interactions Ng et al., Nucleic Acids Res, 2003

43 Pibase database Records domain interactions from PDB and PQS Domains defined with SCOP and CATH All inter-domain and inter-chain distances within 6 Ǻ are considered interacting domains From interacting domain pairs, create list of interfaces with buried solvent accessible area > 300 Ǻ 2

44 Classwork. Go to Pibase website Select largest structural complexes, 1k73, 1i6h Compare two complexes in terms of the number of interacting domains, #interactions per node

45 NCBI CBM database To retrieve interactions: –Record interactions –Use VAST structural alignments to compare binding surfaces –Study recurring domain- domain interactions CBM – database of interacting structural domains exhibiting Conserved Binding Modes Shoemaker et al., Protein Sci, 2006.

46 Definition of CBM Interacting domain pair – if at least 5 residue-residue contacts between domains (contacts – distance of less than 8 Ǻ) Structure-structure alignments between all proteins corresponding to a given pair of interacting domains Clustering of interface similarity, those with >50% equivalently aligned positions are clustered together Clusters with more than 2 entries define conserved binding mode.

47 Number of interacting pairs and binding modes 833 conserved interaction types 1,798 total domain interaction types Up to 24 CBMs per interaction type Classify complicated domain pairs by CBMs Globin example: –630 pairs –2 CBMs account for majority CBMStructuresSpecies 1154Jawed vertebrates 2112Jawed vertebrates 317Clam,earthworm 44lamprey 54V.stercoraria 62Rice,soybeans 72human 82lamprey

48 Classwork. Retrieve structures 1GY3, 1E9H, 1OL2 Examine all interactions within and between chains/domains. How many CBMs do you find?


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