1. BioCote® Antimicrobial Silver Ion Technology Providing proven antimicrobial protection

2. BioCote® is a silver ion antimicrobial additive, it is incorporated into AquaVENT® tubing at the time of manufacture While the term ‘antibacterial’ refers only to bacteria, ‘antimicrobial’ refers to a wider range of organisms including bacteria, moulds, fungi and others. What is BioCote®

3. The Global BioCote® Partner Network Why do these big brands work with BioCote®?

4. Internationally Recognised Endorsed Technology Tried & Trusted Antimicrobial Standard. The BioCote® Brand

5. BioCote® only approves products that achieve a minimum of a 95% reduction in bugs All BioCote® additive performance data is independently generated. BioCote® uses silver which is a safe & naturally occurring BioCote® is a long lasting antimicrobial which will not propagate resistant micro-organisms By partnering with BioCote® you are associated with a branded antimicrobial technology used around the world. Take Home Messages

6. For the lifetime of the product BioCote® will reduce the numbers of microbes, bacteria, mould, and fungi on the product The addition of BioCote® does not affect the performance or appearance of the product in any way BioCote® inhibits the growth of microbes that can cause unpleasant odours or bacterial degradation of the product BioCote® reduces levels of bacteria, such as, MRSA, E.coli and Salmonella on the surface of products by up to 99.9% over a 24 hour period. Useful Phrases

7. Chinese – Silver Chop Sticks Romans – Silver utensils Egyptians – Silver Water Jug Antimicrobial Silver Some History

8. Expected Lifetime Efficacy Broad Spectrum Efficacy Multimodal Action Silver – Anti Microbial Benefits Non Leaching Safe, Natural, Non-toxic

9. Silver Ions Cause Conformation Changes in Microbial Proteins Silver Ions Interfere with DNA Replication Silver Ions Promote Formation of Reactive Oxygen Species. Multi-modal Action of Silver

10. Silver ions combine with microbial proteins located in the cell wall and cytoplasm, which interferes with their normal functioning. These proteins are vital for the healthy functioning of the cell. This loss of function results in an overall inhibition of microbial growth. 1. Silver Ions Cause Conformation Changes in Microbial Proteins

11. Silver ions are known to interfere with the way microbes copy their genetic material or DNA. Microbes, like most living cells, have to make copies of their DNA during reproduction. Silver ions stop the microbes replicating by blocking the copying of their genetic material. 2. Silver Ions Interfere with DNA Replication

12. Silver ions are known to promote the formation of harmful chemicals called reactive oxygen species (ROS) inside microbial cells. ROS are harmful to living cells because they cause significant damage to cell structures. Damage caused by ROS is a major contributor to functional decline, characteristic of aging, that results in further inhibition of microbial growth. 3. Silver Ions Promote Formation of Reactive Oxygen Species

13. Determining Efficacy of BioCote® Treated Surfaces – Independent Study

14. The World Health Organisation estimated an H1N1 pandemic could cost the UK economy over £70 billion. BioCote®- approved silver ion technology is effective at significantly reducing numbers of infectious influenza A H1N1 virus. A measure with the potential to limit the spread of viral infection is worthy of including in an infection control Strategy. H1N1 Study

15. To understand how effective BioCote® approved silver ion antimicrobial technology is against influenza A H1N1 virus when incorporated into various manufacturing materials. BioCote® approved silver ion technology is effective at significantly reducing numbers of infectious influenza A H1N1 virus. H1N1 Study

16. Collaborators

17. Reduction of bacterial contamination in a healthcare environment by silver antimicrobial technology – Journal of Infection Prevention, 2009, vol. 10: 1, 6-12 Campylobacter genotypes from poultry transportation crates indicate a source of contamination and transmission – Journal of Applied Microbiology, 2011, vol. 110: 1, 266-276 Silver ion antimicrobial technology: decontamination in a nursing home – British Journal of Community Nursing, 2009, vol. 14: 6, S25-29. Published Studies

18. To quantify efficacy (performance) of BioCote® treated products in situ* * i.e. in the environments they were designed to operate in Aim of Studies 18

19. Refurbishment at UK acute hospital One ward fitted with BioCote® treated products Comparable ward used as control Normal cleaning operated Processed for total bacterial counts. Reduction of Bacterial Contamination in a healthcare environment by silver antimicrobial technology J. Inf. Prevent. 2009 vol. 10: 1, 6-12.

20. 95.8% Difference 43.5% Difference 92.6% Difference The average CFU count from any BioCote® treated product was 92.6% lower than that from any untreated product in the same environment (unit A) The average CFU count from any BioCote® treated product was 95.8% lower than that from any untreated product in unit B. The average CFU count from any untreated product in unit A was 43.5% lower than that from any untreated product in unit B. Reduction of Bacterial Contamination in a healthcare environment by silver antimicrobial technology Unit A – BioCote TreatedUnit A – Control Ward Treated Products Mean 33 CFU / Swab Untreated Products Mean 447 CFU / Swab Untreated Products Mean 791 CFU / Swab CFU = Colony Forming Unit

21. How do you Protect your Circuits During Non-use?

22. BioCote® inhibits the formation of Biofilm associated with HAI’s BioCote® will reduce the numbers of viruses, bacteria, mould, and fungi on the product BioCote® does not affect the performance or appearance of the product BioCote® inhibits the growth of microbes that can cause unpleasant odours, bacterial degradation or deterioration of the product BioCote® reduces levels of bacteria, such as, MRSA, E.coli and Salmonella on the surface of products by up to 99.9% over a 24 hour period Collaborative studies by BioCote® and virologists have demonstrated the anti-viral properties of BioCote® treated surfaces. Summary

23. Seeing is Believing

25. Contact Us www.armstrongmedical.net info@armstrongmedical.net