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1 Portland Community College Lab Activity 32UrinalysisPortland Community CollegeBI 233
2 FiltrationOccurs in the renal corpuscles: blood pressure forces water and small dissolved molecules to move from the glomerular capillaries to the capsular space and is called the filtrate
3 FiltrateContains mostly water along with excess ions (mostly sodium and potassium), glucose, amino acids and nitrogenous waste products.Lacks RBCs and large plasma proteins.
4 Reabsorption Almost all the water (99%) As well as glucose, amino acids and various ions.These are returned to the blood by passing from the renal tubules to the peritubular capillaries or vasa recta
5 SecretionUnwanted substances such as metabolic wastes, drugs and excess ions (hydrogen and potassium) are removed from the blood and enter the renal tubules.Most occurs in DCT and collecting ducts
6 Urine Normally contains about 95% water Various ions (sodium, potassium, sulfate, calcium, magnesium, bicarbonate)Amino acids, lipids and carbohydratesUrea, uric acid and creatinine
7 Universal precautions You will be examining the physical and chemical characteristics of your own urine sampleWork with your urine onlyWear gloves, safety eyewear, and a maskClean spills with 10% bleach solutionAnything that comes into contact with urine goes into an autoclave bag.
8 Physical Characteristics of Urine Color and transparencyClear, pale to deep yellow (due to urochrome)Concentrated urine has a deeper yellow/amber colorA red or red-brown (abnormal) color could be from a food dye, eating fresh beets, a drug, or the presence of either hemoglobin or myoglobin.If the sample contained many red blood cells, it would be cloudy as well as red.Turbidity or cloudiness may be caused by excessive cellular material or protein in the urine
9 Physical Characteristics of Urine OdorFresh urine is slightly aromaticStanding urine develops an ammonia odorSome drugs and vegetables (asparagus) alter the usual odorElevated ketones smells fruity or acetone-like
10 Physical Characteristics of Urine Specific gravity measures density of urine compared to waterRanges from to 1.0351.001 is dilute1.035 is concentratedIs dependent on solute concentration> is either contaminated or contains very high levels of glucosePatients who have received radiopaque dyes or dextran can also have high specific gravity
11 Chemical Composition of Urine Urine is 95% water and 5% solutesNitrogenous wastes include urea, uric acid, and creatinineOther normal solutes include:Sodium, potassium, phosphate, and sulfate ionsCalcium, magnesium, and bicarbonate ionsAbnormally high concentrations of any urinary constituents may indicate pathology
12 Urinalysis“Dipstick" method: chemical reactions cause color changes on ten different pads on the test strip.LeukocytesNitriteUrobilinogenProteinpHBloodBloodKetonesBilirubinGlucose
13 Dipstick Urinalysis Interpretation pH: large range 4.5 to 8.0 (average is 6.0)The urine pH should be recorded, although it is seldom of diagnostic value.Diet can alter pHAcidic: high protein diet, ketoacidosisAlkaline: vegetarian diet, UTI
14 Dipstick Urinalysis Interpretation Nitrite: Might indicate bacterial infection with gram-negative rods (like E. coli)If bacteria are present, they convert nitrates to nitritesNormal=negative
15 Dipstick Urinalysis Interpretation Bilirubin: indicates the presence of liver disease or biliary obstructionA small amount of bilirubin in urine is normal excessive amounts is calledBilirubinuria: appearance of bilirubin in urineYellow foam when sample is shake
16 Dipstick Urinalysis Interpretation Urobilinogen: Produced in the intestine from bilirubin. Gives feces brown colorNormal=small amountAbsence: renal disease or biliary obstructionIncreased in any condition that causes an increase in production or retention of bilirubinHepatitis, cirrhosis or biliary disease
17 Dipstick Urinalysis Interpretation Leukocytes: Indicates infection or inflammationNormal=negativePyuria: Leukocytes in urineCystitis: Bladder infectionPyelonephritis: Kidney infection
18 Dipstick Urinalysis Interpretation Blood: Almost always indicates pathology because RBC are too large to pass through glomerulusNormal=negativeHematuria: Blood in urinePossible causes: Kidney stone, infection, tumorCaution: Very common finding in women because of menstruation.
19 Dipstick Urinalysis Interpretation Protein: Usually proteins are too large to pass through glomerulus (Proteinuria usually represents an abnormality in the glomerular filtration barrier.)Normal=negativeTrace amounts normal in pregnancy or after eating a lot of proteinAlbuminuria: Albumin in urine
20 Dipstick Urinalysis Interpretation Glucose: In general the presence of glucose indicates that the filtered load of glucose exceeds the maximal tubular reabsorptive capacity for glucose. Normal=negative (can occur temporarily after eating a high carb meal or during stress)Glycosuria: Glucose in urine
21 Dipstick Urinalysis Interpretation Ketones: Intermediate products of fat metabolismUrine testing only detects acetoacetic acid, not the other ketones, acetone or beta-hydroxybuteric acid.Normal=negative or trace amountsKetonuria: ketones in urine(Ketonuria + glucose in urine may indicate diabetes mellitus)
22 Urine sedimentsIf a urine sample is centrifuged, the sediment can be viewed microscopically to examine the solid components.Some solids are normally found in urine
23 Chemical Analysis Sulfates: Normal constituent of urine The urinary sulfate is mainly derived from sulfur-containing amino acids and is therefore determined by protein intake.Phosphates: Normal constituent of urineImportant for buffering H+ in the collecting ductChlorides: Normal constituent of urine.Major extracellular anion.Its main purpose is to maintain electrical neutrality, mostly as a counter-ion to sodium.It often accompanies sodium losses and excesses.
24 sedimentsCells- small numbers of epithelial cells that are shed from various regions of the urinary tract.Large numbers of WBSs and any amount of RBCs are abnormal usually indicate disease
25 Microscopic Examination Pyuria: WBC in Urine Normal:Men: <2 WBCs per hi power fieldWomen: <5WBC generally indicate the presence of an inflammatory process somewhere along the course of the urinary tract
26 Microscopic Examination Hematuria: RBC in Urine RBC's may appear normally shaped, swollen by dilute urine or crenated by concentrated urine.The presence of dysmorphic (odd shaped) RBC's in urine suggests a glomerular disease such as a glomerulonephritis.Crenated RBCDysmorphic RBC
27 Microscopic Examination Epithelial Cells Transitional epithelial cells originate from the renal pelvis, ureters, bladder and/or urethra.Large sheets of transitional epithelial cells can be seen in bladder cancer.Squamous epithelial cellTransitional epithelial cell
28 Microscopic Examination Casts Casts: hardened cell fragments formed in the distal convoluted tubules and collecting ductsForm when cells clump togetherUsually form when urine is acidic or contains high level of proteins or saltsUsually pathological.Can only be seen with microscopic examination
29 Microscopic Examination Epithelial Cells Too many squamous cells: suggest contamination, poor specimen collection
30 White Cell Casts Usually indicates pyelonephritis (kidney infection) Other causes: Interstitial Nephritis (inflammation of the tubules and the spaces between the tubules and the glomeruli. )
31 Red Cell CastsRed blood cells may stick together and form red blood cell casts.Indicative of glomerulonephritis, with leakage of RBC's from glomeruli, or severe tubular damage.
32 Hyaline CastsHyaline casts are composed primarily of a mucoprotein (Tamm-Horsfall protein) secreted by tubule cells.Causes: Low flow rate, high salt concentration, and low pH, all of which favor protein denaturation and precipitation of the Tamm-Horsfall protein.Hyaline Casts appear Transparent
33 Calcium Oxalate Crystals They can occur in urine of any pH.Causes: Dietary asparagus and ethylene glycol (antifreeze) intoxication
34 Uric Acid CrystalsHigh uric acid in blood (by-product of purine digestion/high protein diet)Associated with gout (arthritis)
35 Struvite Crystals Formation is favored in alkaline urine. Urinary tract infection with urease producing bacteria (eg. Proteus vulgaris) can promote struvite crystals by raising urine pH and increasing free ammonia.
36 Chemical Analysis Urea: The end product of protein breakdown Uric acid: A metabolite of purine breakdownCreatinine: Associated with muscle metabolism of creatine phosphate.
37 Microscopic Examination Bacteria Bacteria are common in urine specimens (from contamination)Therefore, microbial organisms found in all but the most scrupulously collected urines should be interpreted in view of clinical symptoms.A = crenated RBC, B = RBC, C = bacteria