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Published byErik Kelley Modified over 9 years ago
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Peter Werner Final portfolio May 30, 2008
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Zoom project
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Zoom images: Morchella elata (morel) asci Zeiss Axioskop, Zeiss Axiocam CCD color images
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More zoom images: Morchella elata (morel) asci Zeiss Axioskop, Zeiss Axiocam CCD color images
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Story project: Mushroom microanatomy
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Asci, Morchella elata (morel), 40x objective, phase contrast Zeiss Axioskop, Zeiss Axiocam CCD image
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Asci, Morchella elata (morel), 40x objective, dark-field Zeiss Axioskop, Zeiss Axiocam CCD image
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Asci, Morchella elata (morel), 10x objective, fluorescence, green (Rhod) ex Zeiss Axioskop, Zeiss Axiocam CCD color image
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Basidium and chrysocystidia, Stropharia rugosoannulata (garden giant mushroom), 63x objective, bright-field Nikon fluorescence scope, Nikon microscope CCD/Nikon Elements composite color image
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Basidium and chrysocystidia, Stropharia rugosoannulata (garden giant mushroom), 100x objective, fluorescence, green (TRITC) ex Nikon fluorescence scope, Nikon microscope CCD/Nikon Elements monochromatic image with color overlay
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Cheilocystidia, Clitocybe saeva (field blewitt), 63x objective, bright- field Nikon fluorescence scope, Nikon microscope CCD/Nikon Elements composite color image
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Various basidiospores: Chroomgompus vinicolor (upper left), 100x obj; Lactarius rubidus (upper right), amyloid (iodine blue) reaction with Melzer’s reagent, 63x obj; Panaeolina foenisecii (bottom), 100x obj; all bright-field. Nikon fluorescence scope, Nikon microscope CCD/Nikon Elements composite color images
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Resolution vs Contrast: Diatom test
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Overview: Gyrosigma balticum (diatom), 63x/0.80 NA objective, bright-field Test of resolution of surface frustules on Gyrosigma Slide by Klaus Kemp Zeiss Axioskop, Zeiss Axiocam CCD black and white images
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Resolution and contrast: NA = 0.9 (condenser aperture wide open) Image well-resolved, but somewhat lacking in contrast
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Resolution and contrast: NA = 0.8 (resoution of condenser matches that of objective) Image well-resolved and with good contrast
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Resolution and contrast: NA = 0.4 (condenser diaphragm at 50% field of view in back focal plane) Image poorly resolved – Airy disk size is such that individual frustules blend together and give the false image of ridges on diatom surface. (“Bad” image)
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Resolution and contrast: NA = 0.05 (condenser diaphragm stopped down to smallest aperture) Image very poorly resolved – Airy disks so large that many points in image run together and there is little resolution. (“Bad” image)
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Plants
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Leaf cross section, Dianthus, 20x objective, phase contrast Pre-prepared slide Olympus CKX41/31
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Anther cross section, Lillium (lily), 4x objective, fluorescence, green (TRITC) ex, red em Pre-prepared slide Olympus CKX41/31
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Stem cross section, Zea mays (corn), 20x objective, fluorescence, green (TRITC) ex, red em Pre-prepared slide Olympus CKX41/31
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Pollen, Pinus (pine), 40x objective, fluorescence, green (TRITC) ex, red em Pre-prepared slide Olympus CKX41/31
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Sporangium, Polystichum munitum (sword fern), 10x objective, fluorescence, blue (FITC) ex Olympus CKX41/31, Nikon Coolpix 4500 digital camera color image, through ocular
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Insects and more diatoms
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Aphid (unidentified species), 4x, dark-field (Ph3 ring substituted for PhL ring) Olympus CKX41/31 (Semi-”bad” image: air bubble and schmutz on otherwise good image)
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Antenna, aphid (unidentified species), 40x, fluorescence, blue (FITC) ex Olympus CKX41/31
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Leg, aphid (unidentified species), 20x, phase contrast Olympus CKX41/31
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Diatom test slide, 20X, bright-field Slide made by master diatom artist, Klaus Kemp Olympus CKX41/31
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Pleurosigma angulatum (diatom), from diatom test slide, 20x, phase contrast Olympus CKX41/31
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Unknowns
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Extra credit and other overlapping stuff
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Make your own slide: all slides were made by me unless otherwise noted. This would constiute at least half the slides presented here. Dark-field: Several, including two darkfield images of morel asci (in both Zoom and Story projects) and one of the aphid images. “Bad” images: two of the diatom slides. Problems with the darkfield aphid slide are also noted.
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