Asci, Morchella elata (morel), 10x objective, fluorescence, green (Rhod) ex Zeiss Axioskop, Zeiss Axiocam CCD color image
Basidium and chrysocystidia, Stropharia rugosoannulata (garden giant mushroom), 63x objective, bright-field Nikon fluorescence scope, Nikon microscope CCD/Nikon Elements composite color image
Basidium and chrysocystidia, Stropharia rugosoannulata (garden giant mushroom), 100x objective, fluorescence, green (TRITC) ex Nikon fluorescence scope, Nikon microscope CCD/Nikon Elements monochromatic image with color overlay
Cheilocystidia, Clitocybe saeva (field blewitt), 63x objective, bright- field Nikon fluorescence scope, Nikon microscope CCD/Nikon Elements composite color image
Various basidiospores: Chroomgompus vinicolor (upper left), 100x obj; Lactarius rubidus (upper right), amyloid (iodine blue) reaction with Melzer’s reagent, 63x obj; Panaeolina foenisecii (bottom), 100x obj; all bright-field. Nikon fluorescence scope, Nikon microscope CCD/Nikon Elements composite color images
Overview: Gyrosigma balticum (diatom), 63x/0.80 NA objective, bright-field Test of resolution of surface frustules on Gyrosigma Slide by Klaus Kemp Zeiss Axioskop, Zeiss Axiocam CCD black and white images
Resolution and contrast: NA = 0.9 (condenser aperture wide open) Image well-resolved, but somewhat lacking in contrast
Resolution and contrast: NA = 0.8 (resoution of condenser matches that of objective) Image well-resolved and with good contrast
Resolution and contrast: NA = 0.4 (condenser diaphragm at 50% field of view in back focal plane) Image poorly resolved – Airy disk size is such that individual frustules blend together and give the false image of ridges on diatom surface. (“Bad” image)
Resolution and contrast: NA = 0.05 (condenser diaphragm stopped down to smallest aperture) Image very poorly resolved – Airy disks so large that many points in image run together and there is little resolution. (“Bad” image)
Make your own slide: all slides were made by me unless otherwise noted. This would constiute at least half the slides presented here. Dark-field: Several, including two darkfield images of morel asci (in both Zoom and Story projects) and one of the aphid images. “Bad” images: two of the diatom slides. Problems with the darkfield aphid slide are also noted.
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