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Project Scope Shootout Stella Breslin Biosc 3 / Cohort 3 Spring 2010 Slide = Chroma Full Skin Section Nuclei stained with Hoechts F-Actin - Alexa 488 Elastin - Cy3 Collegen - Cy5
Chroma Leica Sp5 5/4/10 5/4/10 SBChromaMay4.lif/Image051 mag: 20x Plan Apo 2channels 512x512 AOTF 30% (514) PMT2 519nm - 630nm off 0 HV 827 / AOTF (514) 20% PMT3 638nm - 765nm off -1 HV 750 / line avg 1 frame avg 3 pinhole 30.3 µm pinhole airy 0.5
Leica Spinning Disk 5/15/10 5/15/10 ChromaSD.lif/Series22 HC PL FLUOTAR 10.0x0.30 DRY 3 Channels 1 frame Exp G 932 ms, Red 932 ms, Blue 1000 ms Gain: G 5.9 R 5.9 B 6.3
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Zeiss 2 / DAPI Filter Added 5/11/10 ChromaSlide Multichannel mag: 20x Exp: FITC 346ms Rhod 286ms DAPI 145ms Br -.057,-0.57-,0.66 Con 1.19, 1.06, 1.37 gamma 1.0
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Chroma - Olympus 3- 5/22/10 5/22/10 ChromaSlide mag : 10x Phase exp: 18.6 ms gain 1.0 offset 0 Contrast Stretch Image J
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