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Project Scope Shootout Stella Breslin Biosc 3 / Cohort 3 Spring 2010 Slide = Chroma Full Skin Section Nuclei stained with Hoechts F-Actin - Alexa 488 Elastin - Cy3 Collegen - Cy5
Chroma Leica Sp5 5/4/10 5/4/10 SBChromaMay4.lif/Image051 mag: 20x Plan Apo 2channels 512x512 AOTF 30% (514) PMT2 519nm - 630nm off 0 HV 827 / AOTF (514) 20% PMT3 638nm - 765nm off -1 HV 750 / line avg 1 frame avg 3 pinhole 30.3 µm pinhole airy 0.5
Leica Spinning Disk 5/15/10 5/15/10 ChromaSD.lif/Series22 HC PL FLUOTAR 10.0x0.30 DRY 3 Channels 1 frame Exp G 932 ms, Red 932 ms, Blue 1000 ms Gain: G 5.9 R 5.9 B 6.3
Nikon 20x 5/8/10 Nikon 3 5/8/10 Mag: 20x Exp: TRITC 200ms FITC 500ms DAPI 52ms Contrast Stretch ImageJ
Zeiss 2 / DAPI Filter Added 5/11/10 ChromaSlide Multichannel mag: 20x Exp: FITC 346ms Rhod 286ms DAPI 145ms Br -.057,-0.57-,0.66 Con 1.19, 1.06, 1.37 gamma 1.0
Zeiss 2 / Darkfield 5/11/10 ChromaSlide Darkfield mag: 20x Exp: 130ms Br -0.03 Con 1.6 gamma 1.0
Chroma -Zeiss 2 / Rhod/FITC/DAPI/DIC overlay - 5/11/10 ChromaSlide MC Overlay Mag: 40x Exp: FITC 598ms Rhod 286ms DAPI 24ms Con 1.0 gamma 1.0 DIC exp 4ms / Contrast stretch each channel and DIC overlay done in Adobe PS
Chroma -Olympus 3 - 5/22/10 ChromaSlide Multichannel mag: 10x MC Exp: Red 518.5ms Green 441.9 Offset R -894 G-662 Gain 1.0 Color Merge Contrast Stretch in Adobe PS
Chroma - Olympus 3- 5/22/10 5/22/10 ChromaSlide mag : 10x Phase exp: 18.6 ms gain 1.0 offset 0 Contrast Stretch Image J
Comparisons Slide 6: your succinct comments comparing the images acquired on
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High Speed, Full-Spectrum Spinning-disk Confocal.
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ImageJ An adaptation of NIH image for the Java platform. Can run on any computer systems that can run Java (Sun Microsystems)
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Microscopy Looking at the small stuff. Microscopes “micro” means : Small “scope” means: Watch/ look It was actually developed first as entertainment.
Obj. HC PL APO 10x/0.40 CS Datasheet Material Number: Objective Type:PLAN APO Magnification:10 Numerical Aperture: 0,4 Coverglass:0,17 Immersion:Dry.
Avoiding bleed-through artifacts on the confocal microscope Fluorescent dyes are molecules that, when exposed to light of a specific range of wavelengths,
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Date of download: 7/5/2016 Copyright © 2016 SPIE. All rights reserved. Spatial light modulator-two-photon microscope (SLM-2PM) scheme: (1) Ti:Sa laser.
1 Setting Laser Power and PMT Gain Begin with laser power at 20% Set PMT gain to 6.00.
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An urn contains 1 green, 2 red, and 3 blue marbles. Draw two without replacement. 1/6 2/6 3/6 2/5 3/5 1/5 3/5 1/5 2/5 2/30 3/30 2/30 6/30 3/30 6/30.
Most-to-Least Legible Color Combinations for Viewing on Slide Shows Color and contrast are very important tools in communication. They can be used to enhance.
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Carl Zeiss MicroImaging AIS Solutions for Digital Pathology Andrew Lesniak Director, Product Management.
Local Pollen By Dan Murphy. I started by collecting flowers from different trees and flowers in my neigborhood. After removing the stamens I made slides.
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750LP 780/60 710/ LP 710/50 530/30 Blue Laser (488nm, 100mW)Red laser (644nm,100mW) Alexa 700 APC Cy7 APC-Alexa750 Alexa 488 FITC CFSE SYTOX Green.
Supplementary Figure S1. Hematoxylin and eosin (H&E) staining (left panel) and ASP+ fluorescence labeling (right panel) of adjacent sections containing.
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STORM and PALM How to get started. June 2014 Christa Walther.
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1 Confocal Microscopy David Kelly November 2013 Handbook of Biological Confocal Microscopy. Ed. J. Pawley, Plenum Press Fundamentals of Light Microscopy.
What I Did on My Summer Vacation Olympus IX71 Characterization Substrate Comparison Substrate Cleaning Technique Comparison By: Amelia McCready.
IMMUNODEPLETION OF CONDENSIN FROM XENOPUS EGG EXTRACTS HSSΔcond.Δmockmock boiled beadsextracts cond. anti- cond. anti- α-tub Smc2
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R O Y G B V ellowreenrangelueiolet LONG SHORT ed low energy high energy.
Panel -Distinct segment of comic -Combination of images and text -Sectioned off by lines/borders called frames.
Nikon Eclipse TE2000 Spinning Disc. inverted perfect focus microscope optimized for live cell imaging and time lapse movies Equipped for DIC, Ludl Piezo.
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Fig. 1 (A) (B) (C) * * * * Mock siRNA siRNA Day 3 Day DNA-PKcs Beta actin.
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LYVE-1, CD45, and CD31 staining on frozen sections (5µm) of the mouse prostate The experiments were performed by Scott Gerber & Edith Lord, PhD, University.
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