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Transfusion Systems Transfusion Business CompanyTRANSFUSION CONGRESS Opatia, Croatia June 10-11, 2005 CONFIDENTIAL Programme: - Introduction - How to define.

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Presentation on theme: "Transfusion Systems Transfusion Business CompanyTRANSFUSION CONGRESS Opatia, Croatia June 10-11, 2005 CONFIDENTIAL Programme: - Introduction - How to define."— Presentation transcript:

1 Transfusion Systems Transfusion Business CompanyTRANSFUSION CONGRESS Opatia, Croatia June 10-11, 2005 CONFIDENTIAL Programme: - Introduction - How to define logistics of bloodprocessing - Priority of components - Influence of regulations - Influence of staff related regulations - Influence of platelets production - Example of optimised logistics - TSF separation and bloodbank logistics

2 Transfusion Systems Transfusion Business CompanyTRANSFUSION CONGRESS Opatia, Croatia June 10-11, 2005 CONFIDENTIAL Define priority of bloodcomponents Optimalisation of blood components: Production of ALL components in MOST excellent quality is NOT possible, define priority: - Plasma; quick separation, fast freezing to optimise quality. Freezing < 3 hr after separation - Platelets, storage for 4 – 24 hours, optimal recovery of platelets, high quality. -This choice of most important component together with method of preparation defines basic algoritms of processing, find acceptable consensus for all components.

3 Transfusion Systems Transfusion Business CompanyTRANSFUSION CONGRESS Opatia, Croatia June 10-11, 2005 CONFIDENTIAL Influencing the logistics of blood processing External regulations: - Fractionater; max. delay of freezing 3 hr - GMP; different staf for different departments Internal influences: - Organisational priorities; lab and processing - waste costs; - work during late evening/night; personal costs

4 Transfusion Systems Transfusion Business CompanyTRANSFUSION CONGRESS Opatia, Croatia June 10-11, 2005 CONFIDENTIAL Parameters effecting logistics: method of preparation and choice of bag system. Different Reduction in Lymphocyte and Granulocyte content by removal of BC from fresh and O/N stored blood. (Z.Rácz, Hungary 1997) - Results of separation: Manual (Q), Separator 1 =TACE(Q) and Separator 2=OPTIPRESS) were compaired with these variabels: - Fresh blood and stored (O/N) - Different sizes of BC taken - Distribution of Lymphocytes/Granulocytes were investigated.

5 Transfusion Systems Transfusion Business CompanyTRANSFUSION CONGRESS Opatia, Croatia June 10-11, 2005 CONFIDENTIAL Top and Bottom separation: -Simultanious transfer of Plasma and Red Cells. - Buffycoat is NOT transferred - Hard spin for maximal Plasma recovery, up to 4700g for 15 minutes.

6 Transfusion Systems Transfusion Business CompanyTRANSFUSION CONGRESS Opatia, Croatia June 10-11, 2005 CONFIDENTIAL Conventional Quadruple bag: - Removal of plasma and BC from top of primairy bag. - Transfer of SAGM through same tube. - Quality of components influenced by storage of blood - Platelet production with pooled Single BC or single Dilted BC

7 Transfusion Systems Transfusion Business CompanyTRANSFUSION CONGRESS Opatia, Croatia June 10-11, 2005 CONFIDENTIAL Results of the study: - Stored blood gives better removal of leukocytes with equal volume of BC removed ml BC: FreshStored - Manual Q: 45%70% - Auto Q:60%78% - Auto TAB:71%82%

8 Transfusion Systems Transfusion Business CompanyTRANSFUSION CONGRESS Opatia, Croatia June 10-11, 2005 CONFIDENTIAL Platelet production Platelet production from whole blood: Triple bag conventional: Platelet Rich Plasma, to be separated by an additional spin in PC and PPP. Quadruple bag conventional: A: Diluted buffy coat to be separated by additional spin in PC and a waste residual BC. B: 4 – 6 single buffycoats to be pooled together with a plasma (or additive solution), to produce a pooled random platelet concentrate. Buffycoats to be pooled within 24 HOURS

9 Transfusion Systems Transfusion Business CompanyTRANSFUSION CONGRESS Opatia, Croatia June 10-11, 2005 CONFIDENTIAL Basic Processing procedures Platelet production from whole blood: Quadruple TAB bag : Diluted buffycoat to be separated by additional spin in PC and a waste BC. Better results as quadruple Dil BC Triple TAB bag: Single buffy coat to be pooled together with 4-6 and a plasma (PAS etc), to produce a pooled random platelet concentrate. Buffycoats to be pooled within 24 HOURS

10 Transfusion Systems Transfusion Business CompanyTRANSFUSION CONGRESS Opatia, Croatia June 10-11, 2005 CONFIDENTIAL Basic Processing procedures PERFORMANCE OF SEPARATION TABCONV QTriple PRP Plasma recovery L-reduction RCC+++++/+- Platelet recovery Separation time2 – 7 min2 – 3 min2 Spin Syphonage effectyesnono Disposable cost++++++

11 Transfusion Systems Transfusion Business CompanyTRANSFUSION CONGRESS Opatia, Croatia June 10-11, 2005 CONFIDENTIAL QUALITY OF DIFFERENT COMPONENTS

12 Transfusion Systems Transfusion Business CompanyTRANSFUSION CONGRESS Opatia, Croatia June 10-11, 2005 CONFIDENTIAL Optimal separation logistics in Rotterdam (NL). - Collection of blood: every day from 09:00 untill 21:00. The collected blood is stored O/N in a incubator of 22°C. - Test samples of blood are tested during the night from 00:00 untill 07:00. -Test results are used to release the collected blood before the processing starts at 08:00. - Unsafe blood is processed separate of safe blood. - Pooling of Buffycoats is always done with safe products, no waste of pools due to positive test results.

13 Transfusion Systems Transfusion Business CompanyTRANSFUSION CONGRESS Opatia, Croatia June 10-11, 2005 CONFIDENTIAL Alternative for platelet production: Terumo Saving Platelet filter 3 leukodepleted products RBC, PLASMA, PLTS in 1 LGF version: results in small “BC” Some recent tests done:

14 Transfusion Systems Transfusion Business CompanyTRANSFUSION CONGRESS Opatia, Croatia June 10-11, 2005 CONFIDENTIAL Results of TSF: Elverum; Norway (5000 col year ) Pool of 4, with T-SOL (BC 24hr old) Buffycoat: weight is gr netto. Redcell loss: ml in BC. Diluted BC 10 ml plasma. Pool: Volume of ML, HcT= 21 – 23 % (recovery of pooling: 97%) Results: Pooled 6 pools, Plt recovery: 294 – 356 x 10 9 unit Recovery of second separation: 75 – 93% of pool

15 Transfusion Systems Transfusion Business CompanyTRANSFUSION CONGRESS Opatia, Croatia June 10-11, 2005 CONFIDENTIAL Results of TSF: Maribor, Slovenia: 9 – 13 May Pool of 5 BC with PLASMA Buffycoat: weight is gr netto. Redcell loss: ml in BC. Diluted BC 10 ml plasma. Pooled buffycoats of different ages: -Overnight stored -Two days stored (aggitated) -Three and four days stored (aggitated)

16 Transfusion Systems Transfusion Business CompanyTRANSFUSION CONGRESS Opatia, Croatia June 10-11, 2005 CONFIDENTIAL Results of TSF: Maribor, Slovenia: 9 – 13 May Fresh pooled: (O/N): 240 – 380 Day 2: 342 and 348 Day 3: 332 and 93 (cherry coloured BC’s!!) Day 4: still under investigation. Recovery of pooling: 99% Recovery of sec separation: 75% (1950 rpm 4 m 30 sec) % (1300 rpm for 7 min, acc 8, brake 1)

17 Transfusion Systems Transfusion Business CompanyTRANSFUSION CONGRESS Opatia, Croatia June 10-11, 2005 CONFIDENTIAL New posibility for TSF saving Platelets Small centres, willing to pool BC, allowing them to have prestorage filtering conditions with pooling untill day 3 (or more) to optimise the logistics of the platelets.

18 Platelet pooling with BP-KIT + IGIIII-S PL Filter holder += BPKIT with IGIII S-PL –Buffy-coat pooling kit with soft housing platelet filter High yield leukodepleted platelet conc.


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