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Biology 116-Biotechnology Ralph M. Sinibaldi, Ph.D..
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Course Goals Technical training for research, development or production positions in biotech Conceptual training in molecular biology and biotechnology Biotech Industry overview Soft skill training Resumes Interviews Project teams and teamwork
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Learning Outcomes Describe the science of biotechnology and identify its product and company domains Give examples of careers and job responsibilities associated with biotechnology Understand and apply safety considerations and lab etiquette Describe how scientific methodologies are used to conduct experiments and develop products Understand and apply rules of documentation and intellectual property Describe what intellectual property is and why it is important in biotechnology Understand regulatory compliance and what agencies are responsible for it Describe the Human Genome project and be able to discuss its implications
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Vocabulary Insulin – a protein that facilitates the uptake of sugar into cells from the blood DNA – abbreviation for deoxyribonucleic acid, a double-stranded helical molecule that stores genetic information for the production of all of an organism’s proteins Recombinant DNA (rDNA) technology – cutting and recombining DNA molecules Polymerase chain reaction (PCR) – a technique that involves copying short pieces of DNA and then making millions of copies in a short time Cloning – method of asexual reproduction that produces identical organisms Fermentation – a process by which, in an oxygen-deprived environment, a cell converts sugar into lactic acid or ethanol to create energy Diabetes – a disorder affecting the uptake of sugar by cells, due to inadequate insulin production or ineffective use of insulin Proteases – proteins whose function is to break down other proteins Antibodies – proteins developed by the immune system that recognize specific molecules (antigens) Pharmaceutical – relating to drugs developed for medical use
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Vocabulary Research and development (R&D) – refers to the early stages in product development that include discovery of the structure and function of a potential product and initial small- scale production Pure science – scientific research whose main purpose is to enrich the scientific knowledge base Virus – a particle containing a protein coat and genetic materials (either DNA or RNA) that is not living and requires a host to replicate Applied science – the practice of utilizing scientific knowledge for practical purposes, including the manufacture of a product NIH – abbreviation for National Institutes of Health; the federal agency that funds and conducts biomedical research CDC – abbreviation for Centers for Disease Control and Prevention; national research center for developing and applying disease prevention and control, environmental health, and health promotion and education activities to improve public health DNA fingerprinting – an experimental technique that is commonly used to identify individuals by distinguishing their unique DNA code
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“New technology is neither inherently good or harmful, this is determined by how man chooses to use the technology”
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What is Biotechnology? Biology Technology
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Defining Biotechnology Biotechnology is defined as the study and manipulation of living things or their component molecules, cells, tissues, or organs.
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Biotechnology Business and Business Strategy
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Fact Most new Biotech Companies Ultimately Fail
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Domains of Biotechnology. The major domains of biotechnology include 1) industrial and environmental; 2) medical/pharmaceutical; 3) agricultural; and 4) diagnostic/research
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Types of Companies Product Development Advantages –Therapeutic products with large markets –Patent protection –High gross margins Disadvantages –High risk –Long development times Platform Technologies Advantages –Shorter development times –Lower risk Disadvantages –Highly competitive with ever changing technology
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Reagent Advantages –Short development time –High profit margins Disadvantages –May not be proprietary –Manufacturing costs driven Service Advantages –No manufacturing –Can be highly profitable Disadvantages –Can underestimate costs Types of Companies
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Type of companies Equipment or Instruments Advantages –Proprietary –Can bundle with associated reagents Disadvantages –Significant capital investment –Lower margins on instruments
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Starting a Company An Idea or a Technology Projected product(s) or service(s) Market Analysis Business Plan Funding Seed round –Friends & Family –Early Venture Capital Investor –Angel Investor(s) “A” round –Venture Capital –Angel Investors “B” Round –Venture Capital –Corporate Investors or Partners “C” Round Exit Strategy –IPO or Acquisition
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Business Plan Summary-two pages Market Opportunity Company background- stage & type Market Market analysis Competitors Technology Proof of concept Similar technologies Expert opinions Intellectual property Patent applications Potential conflicts Development Plan Marketing Plan Distribution Management Org chart Bios of Principals Appendices
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Role of People Corporate structure Skill base of employees Building the right team Human resources system
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Technology Publications Patents Proof of concept for components Breadboard Full Working prototype
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Types of Finance Debt Financing Loans Credit Equity Financing Private stock –Friends & family –Private investors –Angel Investors –Venture Capital funds –Corporate partners
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Other Sources of Funding Grants SBIR –NIH, NSF, USDA, NASA, NIST Stage I- $100,000.00 Stage II- $750,000.00 to 1 million –ATP- 2 million up to 32 million –DARPA- national defense applications Corporate partnerships Marketing & Distribution relationship Equity
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What appeals to investors Technology Business Plan Management Team Multiple Products
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Compensation Salary Bonus -10 to 30 % of salary Must achieve aggressive goals Stock options Founder’s Employee
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Corporate Structure Hierarchical
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Corporate Structure-Matrix
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Corporate Structure-Hybrid Hierarchical & Matrix Combined Departmental Organization Multidisciplinary Project Teams
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Decision Making Technology-based Research Manufacturing Resource-based Marketing-based
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Sustainable Business Reducing Chances Large and Unpredictable Capital Requirements Long Product Development Cycles Regulatory Issues with Product Rapidly Changing Market Forces High Probability of Late Stage Product Failure Rare Instances of Sustained Profits Increasing Chances Capital Requirements Kept Low Well-Defined, Predictable Business Milestones Clear, Market-Oriented Business Plan Critical Mass to Successfully Compete Experienced Management Relevant to Strategy Being Pursued
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Evolution of Company Production-based Technology-based Market-based
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Marketing SWOT analysis Strengths Weaknesses Opportunities Threats
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Safety
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Safety Values Safety is not just a priority but a value Safety is an unwritten rule, a special norm, the workers should follow in all circumstances It is a value that is never questioned or compromised
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Safety Habits Safe for you and me Prevent accidents by noticing at-risk situations and behaviors Live safely at home, at work, and everywhere you go Teach an attitude, promoting safety
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Personal Safety Right to work in a safe workplace Responsibility Protect your circle of safety and know how it may influence others Illness and Injury prevention program
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Work Environment Organize safety for everyone Remove tripping hazards Do not store heavy items up high where they may fall Do not rush or run in the workplace Cleanup any liquid spills immediately Report any potential hazards
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Stress can lead to accidents Recognize personal burn-out Get enough sleep Get professional help Respect emotions of coworkers Develop active listening skills Develop positive, healthy relationships with coworkers
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Emergencies Medical response Earthquake Fire Chemical spills Regional disasters
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What to do Know emergency numbers-911 etc. Be prepared and have a plan Follow plan Stay calm Consider immediate need and response Communicate with others Know safety procedures, tools & escape routes
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Neighborhood or regional disaster Home communication plan Know alternative routes Know who are your neighbors Be a good citizen You may have to stay where you are
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Emergency Evacuation Plan Assist those who need help to get to the protected area Know who is present and absent Communicate with other tenants Be prepared for first aid and medical responses
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Medical responses Immediate first aid Notify response teams, call 911 Provide assistance and comfort Transport to trauma or urgent care facility
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Earthquake Safety Stay calm, shield yourself from falling objects Prevent falling objects by storing heavy objects low and tie down equipment Keep aisles and routes clear Follow evacuation plan
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Fire Safety Report fires immediately-response time is critical Know locations of fire fighting equipment Extinguishers Fire blankets Fire alarm Know when to evacuate & get everyone out If smoke is present stay low, crawl if necessary Know evacuation route
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Fire Extinguishers Classification A- Ordinary combustible B- Flammable Liquid C- Electrical D- Combustible metal P-A-S-S Pull-Aim-Squeeze-Sweep Aim at the base of the fire and sweep Limited time and quantity of extinguishing material
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Personal Protection Actively work to prevent & avoid accidents Protect working space Protect coworkers Secondary containment- create boundaries & layers of safety appropriate for conditions and scale of work
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Working with hazards Create a safety zone, CONTAIN Know the hazard, PROTECT Protect yourself Protect those around you Protect environment around you Safe to touch, DECONTAMINATE Secondary & tertiary zones reduce the chances of injury or disaster
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Personal safety attire Lab coat Safety glasses Closed-toed shoes Gloves when appropriate
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Chemical safety Know the hazards-MSDS sheets Specialized training may be necessary Proper storage of chemicals Use proven well thought-out protocols Additional personal protection attire may be required Face shield Chemical goggles Latex gloves and aprons Additional shielding Adequate ventilation Proper disposal of chemicals
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Radiation safety Proper training Shielding Monitoring equipment Geiger counter Wipe tests Proper storage and disposal of radioactive materials
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Radiation Safety Commonly used isotopes 14 C, 35 S, 32 P, 3 H, 125 I, 131 I Geiger counters Different probes Scintillation Counters Radiation exposure badges
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Lab Etiquette & Lab Operation
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Common Courtesy Do not use the last of a reagent and not replace it Do not use other people’s equipment and reagents without asking Keep your work area and common work areas clean and orderly Do not play the radio/music without consulting others in the work area Be willing to work as a team on all projects Dress appropriately including avoiding excess perfume/cologne
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Levels of Operation Sterile reagents Liquids autoclave at 121º C for 15-20 minutes using slow exhaust. Alternatively, reagents can be filter-sterilized using a 45 or 22 micron filter Glassware autoclaved and cover with aluminum foil. Plastic ware is sterile Bottles/reagents may be needed to be flamed when opened or opened in a sterile environment (laminar flow hood) RNase-free Liquids sterilized for 1 hour or made with Rnase-free reagents and solvents. Glassware treated in an oven for several hours and covered with foil Reagents must be RNase-free Clean room conditions Dress and garb appropriately for the level of clean room May include no makeup and cologne
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Documentation
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Documentation System Corporate Policy & Procedures Department Policy & Procedures Quality System Requirements Management Control Traceability, Records & Archival
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Quality System Each manufacturer shall establish and maintain a quality system that is appropriate for the specific medical device(s) designed or manufactured, and that meets the requirements of this part
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Quality System Requirements Management responsibility Quality Policy- commitment to quality that is understood, implemented and maintained at all levels Organization- assigned responsibility and independent authority, adequate resources, effectively establish, effectively maintain, review, quality plan, quality procedures Quality Audit- independent & documented Personal- qualifications & training Made aware of device defects which may occur from improper performance of theirs specific jobs Made aware of defects & errors in verification & validation
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Quality System Subparts Subpart B- Quality system requirements Subpart C- Design controls Subpart D- Document controls Subpart E- Purchasing controls Subpart F- Identification & traceability Subpart G- Production & process controls Subpart H- Acceptance activities
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Quality System Subparts Subpart I- Nonconforming product Subpart J- Corrective & preventive action Subpart K- Labeling & packaging control Subpart L- Handling, storage,distribution and installation Subpart M- Records Subpart N- Servicing Subpart O- Statistical techniques
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Subpart D- Document Controls Each manufacturer shall establish and maintain procedures to control all the documents required. The procedures shall provide for the following Shall designate an individual(s) to review for adequacy and approve prior to issuance Date and signatures of approval Available where needed and obsolete documents removed Changes reviewed, approve, documented, described, recorded, identity documents affected,communicated and effective date noted
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What is a Document? Legal perspective- any scrap of paper that has written information A memo, email,letter, note, meeting minutes Notebook entry, patent application, report Plan, protocol, written instruction, procedure, policy statement Label, tag, placard, sign, flowchart, blueprint,design description Formal documentation, contracts, licenses, publications, marketing ads, regulatory submissions
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Document Chain Quality requirement, quality procedure, corporate policy, Mfg process, records of work, history files, legal contracts, Dept specific procedures, communication, personnel, training, reports, etc. Request forms, Drafts, revision control, Identification, approval process, signatures,dates, archival, accessibility
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Material Chain Acceptable design and supply, vendor, identity, purchasing, receiving, inspection, acceptance, raw material, storage inventory, use, in-process, finished good, labeling, packaging, qualification, storage, distribution, customer, non-conformance, complaint,retention practices, disqualification, disposition, records
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Subpart M- Records All records required by this part shall be maintained at the manufacturing establishment or other location that is reasonably accessible to responsible officials of the manufacturer and to employees of FDA designated to perform inspections Such records, including those not stored at the inspected establishment, shall be made readily available for review and copying by FDA employees Such records shall be legible and shall be stored to minimize deterioration and to prevent loss Those records stored in automated data processing systems shall be backed up
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Confidentiality & Retention The firm should be encouraged to mark records they feel are confidential to assist the FDA in determining what information may be disclosed under the freedom of Information Act (FOIA) Impress upon the manufacturers that marking all copies of records and documents confidential does not aid the FDA in making its FOIA determination Records required by the QS/GMP must be retained by the manufacturer for a period of time equivalent to the design and expected life of the device, but in no case less than 2 years from the date of release for commercial distribution by the manufacturer
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Records and Reports Final report Name & address of facility performing study Objective and procedures in approved protocol Statistical methods, transformation of data, calculations Test articles & control articles (include stability), test system, dosage Describe circumstances that may affect quality & integrity of data Name study director, other professionals, scientists Signed and dated reports of each individual Location of data & records, specimens, final report QA statement of completion Signature of study director Amendments to report, signed Storage, retention, retrieval, of records & data, specimens
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Notebook Entry Title, date, who, witness (legal, patent) Purpose, materials & methods TRACEABILITY- Identify equipment, and source of materials & protocols used Factual Statements for observations and conclusions Avoid unsupportable claims or leading suggestions for follow-up
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Development Report Title, project identity, investigators, date, distribution Summarize, show linkage to records Objective and outcome Protocol & test methods The facts- results and conclusions The importance (simple and realistic)
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Validation Report Title and identity, controlled document Reference approved validation protocol Object and outcome, clear conclusion Was the method, process, product validated? How? Results vs acceptance parameters Archive record, design history file
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How to use documents Use approved, effective documents, or documents identified for approved protocols Follow the procedure Indelible ink (black), legible, in designated fields for entering information No extraneous entries!! Record deviations from procedure by creating separate document Sign and date The job is not finished until documented!
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Technical Writing DELIVER THE MESSAGE- communicate the objective, scope and outcome DELIVER THE HOW- communicate the means, source of records, raw data and conclusions DELIVER THE SO WHAT- communicate the importance of the findings, the relevance to the business, project, process or system
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Intellectual Property and Compliance
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Intellectual Property Laboratory notebooks Content & Witnessing Disclosures of invention Priority dates Confidential Information Trade secret vs Patent Patents –Compositions of matter, Process or procedure, Articles of Manufacture, Machines and Improvements Types of Patents –Utility, Design and Plant Patent Criteria –Conception, Reduction to practice, Utility, Novelty, & Obviousness
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Proper Research Notebooks Physical requirements Bound notebook ( no removable pages) Permanent ink ( Blue or Black) Content Purpose of experiment Materials and Methods Results –Pictures and graphs pasted in have to be signed across Discussion and Conclusions New inventions are recorded Witnessing Who should witness and how often?
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Witnessing Lab Notebooks Who ? Someone familiar with the research –It should not be a colleague working on the same project –Why not? They may be an inventor if they have contributed know how How often? Every week or two weeks
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Disclosure of Invention Some companies require as the second step in pursuing a patent Refers to initial notebook entry Can include a brief mention of related technology and prior art Who is the inventor or inventors? Inventors must contribute to the conception of the idea People or staff who perform the experiments are not inventors unless they contribute intellectually
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Trade Secret or Patent Trade secret When the process or formulation is not novel When it can be easily used by competitors without the knowledge of inventor Can last indefinitely Patenting is publishing exactly how something is made or produced Patent to protect the inventor from others using his invention or idea Patents can be licensed to others for a fee and/or royalty Patents are not intended to create a monopoly Patents last 20 years
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What can be patented Compositions of matter A new chemical entity produced from a combination of two or more compounds –Common in agricultural & pharmaceutical research Process or procedures A series of steps that are followed to synthesize a new compound or make a new product Articles of manufacture Nearly every man-made object Machines Any mechanical or electrical apparatus/device Improvements on any of the previous
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Types of Patents Utility Patent Most common and most difficult Functional characteristics of machines, devices, compounds Exhaustive description of how to make and use the invention including drawings Duration is 20 years Design Patent Protects the shape and ornamental design of an article 14 year duration Plant Patent New plant variety awarded for 20 years
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Patent Criteria Conception Formulation of the invention detailed enough to allow a person knowledgeable in the field to make and use the invention Reduction to practice Inventor makes or constructs the invention to demonstrate its usefullness Utility Invention must be useful or have utility Novelty or prior art Must not be a copy or a repetition of an existing invention Obviousness The invention should not be obvious to some one well-practiced in the field
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Filing a Patent Filing fee The applicant is required to pay a fee for the processing of the application Search & examination The examiner will conduct a prior art search to ascertain novelty and evaluate the claims to establish the scope of the invention Publication Sucessful applications will be published Maintenance fees Applicant must pay periodic maintenance fees
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Parts of a Patent Title Inventors Assignee- the company or entity who is assigned ownership of the patent Abstract Summary of invention Detailed description of invention Figures and drawings Claims Establish scope of the invention
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Patent Strategy Patenting life forms and genes Easier following 1980 US Supreme court ruling, Diamond vs Chankrabarty Reach-through patents Patenting of genes based on their sequence but having no idea about their function Patent stacking Situation where more than one scientist has filed a patent on a gene
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Making Money on Patents Assignment- patent or patent application of invention can be sold or assigned to another party License- the patent may be licensed to another party. This may include a licensing fee and royalties Cross licensing- a situation where multiple patents cover the same or similar areas exist and the owners of such patents may have to cross license each other’s patents to exploit the invention
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Regulatory Compliance
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US agencies & their roles Food and Drug Agency (FDA) –GLP and GMP –Standard Operating Procedures (SOPs) United States Dept of Agriculture (USDA-APHIS) Environmental Protection Agency (EPA) National Institutes of Health (NIH) –Office of Recombinant DNA Drug Development GLP GMP ISO 9000
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US Regulatory Oversight in Biotech AgencyProducts Regulated US Dept of Agriculture Plant pests, plants and veterinary biologics Environmental Protection Agency Microbial/plant pesticides,new uses of existing pesticides, novel microorganisms Food and Drug Administration Food, feed, food additives, veterinary drugs, human drugs, medical devices, diagnostics
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USDA and APHIS APHIS is authorized to regulate the interstate movement importation and field testing of organisms and products altered or produceds through biotech processes that are plant pests or suspected of being so. Permit for movement and importation –Organism, origin and its intended use Permit for release into environment –Oversight of field testing of biotech products Genes and gene products, origin, purpose of test, experimental design,and precautions to prevent escape Courtesy permits –Involves non regulated plants –Can involve intrastate movement
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FDA Unexpected effects- unexpected genetic effects Known toxicants Nutrient level Allergenicity New Substances Antibiotic resistance selectable marker Plants developed to make specialty nonfood substances Issue specific to animal feed
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Research and Development Vocabulary Reagent – chemical used in an experiment Efficacy – the ability to yield a desired result or demonstrate that a product does what it claims to do Large-scale production – the manufacture of large volumes of a product Clinical trials – a strict series of tests that evaluates the effectiveness and safety of a medical treatment in humans FDA – abbreviation for the Food and Drug Administration; the federal agency that regulates the use and production of food, feed, food additives, veterinary drugs, human drugs, and medical devices Cystic fibrosis (CF) – genetic disorder that clogs the respiratory and digestive systems with mucus Therapeutic – an agent that is used to treat diseases or disorders EPA – abbreviation for the Environmental Protection Agency; the federal agency that enforces environmental laws including the use and production of microorganisms, herbicides, pesticides, and genetically modified microorganisms USDA – abbreviation for United States Department of Agriculture; the federal agency that regulates the use and production of plants, plant products, plant tests, veterinary supplies and medications, and genetically modified plants and animals
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Good Laboratory Practice (GLP) A very consistent way of performing and documenting research & development work All documented experiments are performed in a consistent fashion and are witnessed in a timely and consistent fashion Procedures are validated Reagents are validated and listed Instruments and equipment that are utilized in experiments are routinely calibrated and validated FDA monitored
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Good Manufacturing Practice (GMP) All procedures used in manufacturing are consistent, fully validated and witnessed Use Standard Operating Procedures (SOPs) Reagents, chemicals and equipment are specified, validated and calibrated Testing equipment specified and routinely calibrated Some drugs need to be produced in a sterile environment The sterility of the manufacturing environment needs to be monitored and documented FDA monitored
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Standard Operating Procedure Detailed specific protocol Steps may be monitored or witnessed Reagents specified Grade Source or manufacturer Equipment specified Manufacturer Model number Equipment calibration Calibration method Calibration frequency Calibration log Calibrations are witnessed
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Iso 9000 or above Standard ways of doing business and documenting it In addition to manufacturing practices it can include Shipping Maintenance of plant and equipment Order taking Customer and technical service Handling of complaints Communications Needed for world marketing and distribution
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Scientific Method Codefined and promoted in 17 th century by Rene Decartes and Francis Bacon Steps involved in scientific method Make observations Ask questions Make educated guesses about possible answers Base predictions on the guesses Devise ways to test predictions Draw conclusions
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Scientific Method Hypothesis – “educated guess” based on observations and questioning Predicted result occurs – hypothesis is most likely correct Individuals using scientific method should be objective and unbiased
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The Scientific Method
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Scientific Method Original HypothesisDevise method to test hypothesis Analyze results Results support hypothesis Results support hypothesis but suggest minor refinements Results are so unexpected that they do not support original hypothesis and require a new hypothesis Results do not support original hypothesis but fall within range that could be expected if original hypothesis is slightly modified Retest using minor refinements of process Test using slightly modified hypothesis Test new hypotheses
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Observe Observe Ask Questions Ask Questions Formulate Hypothesis Formulate Hypothesis Derive Predictions Formulate Hypothesis Formulate Hypothesis Derive Predictions Test Hypothesis Test Hypothesis Perform Experiments Analyze Data Test Hypothesis Test Hypothesis Perform Experiments Analyze Data Evaluate outcome Evaluate outcome Hypothesis supported Hypothesis supported Curiosity satisfied Curiosity satisfied Move onto another topic Move onto another topic No New Hypothesis New Hypothesis
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Scientific Method & Experimental Design Testable hypothesis One variable at a time Positive controls Negative controls Background determinations Data Normalization
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Human Genome Project
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The Human Genome Project Determining the human DNA sequence Understanding the function of the human genetic code Identifying all of the genes Determining their functions Understanding how and when genes are turned on and off throughout the lifetime of an individual
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HGP (1990 – 2003 ) Participants US DOE NIH UK Medical Research Council and Wellcome Trust, UK 18 countries including France, Japan, Germany and China
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Goals of HGP 1. Identify all the approximate 25,000 genes in human DNA. 2. Determine the sequences of the 3 billion chemical base pairs that make up human DNA. 3. Store this information in databases 4. Improve tools for data analysis, 5. Transfer related technologies to the private sector and 6. Address the ethical, legal, and social issues (ELSI) that may arise from the project.
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Methodology DNA Source Mapping Genetic Linkage Map Physical Map DNA sequencing Clone by clone sequencing Whole Genome Shotgun sequencing Assembling
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Genetic Linkage Map Distance between markers (genes) are determined by meiotic recombinational frequencies between the markers (or genes). Gives only an estimate of the distance between markers or genes Unit of measurement – cM (centiMorgans)
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Construction of Genetic Linkage Map
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Physical Map Constructed from information obtained from the chemical characteristics of the DNA itself and not from the genetic recombination analysis. Unit of Measurement – bp (basepair). Hence, more precise and exact in pinpointing the location and distance of the genes.
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2 Types of Physical Maps Low resolution Chromosomal (Cytogenetic) map cDNA map High resolution Top-Down Mapping Bottom-up Mapping
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Top DownBottom Up
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Genetic Map VS. Physical Map
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Automated sequencers: ABI 3700 and MegaBACE 96–well plate robotic arm and syringe 96 glass capillaries load bar
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AUTOMATED SEQUENCE GEL
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AUTOMATED DNA SEQUENCE
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Methodology DNA Source Mapping Genetic Linkage Map Physical Map DNA sequencing Clone by clone sequencing Whole Genome Shotgun sequencing Assembling GigAssembler
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Result by the numbers The human genome contains 3164.7 million chemical nucleotide bases (A, C, T, and G). The average gene consists of 3000 bases, but sizes vary greatly, with the largest known human gene being dystrophin at 2.4 million bases. The total number of genes is estimated at 20,000 to 25,000— much lower than previous estimates of 80,000 to 140,000. Almost all (99.9%) nucleotide bases are exactly the same in all people. The functions are unknown for over 30% of discovered genes.
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Results Contd. Less than 2% of the genome codes for proteins. Repeated sequences that do not code for proteins ("junk DNA") make up at least 50% of the human genome. Repetitive sequences are thought to have no direct functions, but they shed light on chromosome structure and dynamics. During the past 50 million years, a dramatic decrease seems to have occurred in the rate of accumulation of repeats in the human genome.
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Genome Facts The human genome's gene-dense "urban centers" are predominantly composed of the DNA building blocks G and C. In contrast, the gene-poor "deserts" are rich in the DNA building blocks A and T. Genes appear to be concentrated in random areas along the genome, with vast expanses of non-coding DNA between. Stretches of up to 30,000 C and G bases repeating over and over often occur adjacent to gene-rich areas, forming a barrier between the genes and the "junk DNA.“ Chromosome 1 has the most genes (2968), and the Y chromosome has the fewest (231).
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AreaGoalAchievedDate Achieved Genetic Map2- to 5-cM resolution map (600 – 1,500 markers) 1-cM resolution map (3,000 markers) September 1994 Physical Map30,000 STSs52,000 STSsOctober 1998 DNA Sequence95% of gene-containing part of human sequence finished to 99.99% accuracy 99% of gene-containing part of human sequence finished to 99.99% accuracy April 2003 Capacity and Cost of Finished Sequence Sequence 500 Mb/year at < $0.25 per finished base Sequence >1,400 Mb/year at <$0.09 per finished base November 2002 Human Sequence Variation 100,000 mapped human SNPs 3.7 million mapped human SNPs February 2003 Gene IdentificationFull-length human cDNAs15,000 full-length human cDNAs March 2003 Model OrganismsComplete genome sequences of E. coli, S. cerevisiae, C. elegans, D. melanogaster Finished genome sequences of E. coli, S. cerevisiae, C. elegans, D. melanogaster, plus whole-genome drafts of several others, including C. briggsae, D. pseudoobscura, mouse and rat April 2003
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Endeavors after HGP Transcriptomics - involves large-scale analysis of messenger RNAs transcribed from active genes to follow when, where, and under what conditions genes are expressed. Proteomics - can bring researchers closer to what's actually happening in the cell than gene-expression studies. Structural genomics - initiatives are being launched worldwide to generate the 3-D structures of one or more proteins from each protein family, thus offering clues to function and biological targets for drug design. Comparative genomics - analyzing DNA sequence patterns of humans and well-studied model organisms side-by-side—has become one of the most powerful strategies for identifying human genes and interpreting their function.
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Summary of Human Genome Project Introduction: Background and History of HGP (1990-2003) Methodology: DNA source Genome Map Genetic Linkage Map Physical Map – Low Resolution and High Resolution DNA sequencing Clone-by-clone sequencing Whole Genome shotgun sequencing Assembling GigAssembler Results: Completed Human Genome Sequencing Identified 15,000 genes Future: Applications in the field of Medicine, Forensics, Environment etc., Further research in the fields of Transcriptomics, Proteomics, Structural and Comparative Genomics
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Resourceful information available at: DOE website – www.doegenomics.org National Human Genome Research Institute – www.genome.gov Joint Genome Institute – www.jgi.doe.gov National Center for Biotechnology Institute – www.ncbi.nlm.nih.gov
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