1. Muscles II: Microscopic Anatomy and Contraction October 31 – Nov 3 2014

2. Muscle Structure Muscle Fascicle (bundle of fibers) Muscle Fiber (single cell) Myofibril Sarcomere (unit of contraction)

3. Microscopic Anatomy of Skeletal Muscle Large, cylindrical, multinucleate cells Contain many mitochondria; nearly filled with myofibrils Some organelles have unique vocabulary: –Sarcolemma: cell membrane –Sarcoplasm: cytoplasm –Sarcoplasmic reticulum: modified ER, surrounds each myofibril; store Ca 2+

4. Each myofibril can be divided into contractile units called sarcomeres. Sarcomeres consist of overlapping protein filaments of actin and myosin. Regular arrangement of dark and light bands. Dark bands occur where myosin is present. Microscopic Anatomy of Skeletal Muscle

5. The M line is where the myosin attaches Z discs (a membrane) mark the edge of each sarcomere; serve as attachment site for actin Microscopic Anatomy of Skeletal Muscle

6. Use the picture to come up with a definition of the following: I band A band H zone Microscopic Anatomy of Skeletal Muscle

7. Use the picture to come up with a definition of the following: I band – area without myosin fibers; aka light band A band – area with myosin fibers; aka dark band H zone – area without actin fibers Microscopic Anatomy of Skeletal Muscle

8. First match the words … actincell myofibrilgroup of cells sarcomerecell membrane fascicleprotein muscle fiberorganelle sarcolemmacontractile unit Then, write a paragraph that uses all the words in both columns above and explains that structure of the muscle. Turn & Talk

9. Contraction Overview Globular heads of myosin filaments attach to actin filaments. Myosin pulls actin filaments : “Sliding filament theory” Causes sarcomere to shorten, particularly the light bands

10. Contraction Overview Globular heads of myosin filaments attach to actin filaments. Myosin pulls actin filaments : “Sliding filament theory” Causes sarcomere to shorten, particularly the light bands light dark light

11. Contraction Overview Which shows contracted muscle fibers? How can you tell?

12. Contraction Overview Relaxed muscle Contracted muscle Relaxed muscle Contracted muscle (large light bands) (small light bands) (large light bands) (small light bands)

13. Contraction Overview What are these? What are these? See animationSee animation! Mitochondria

14. Contraction Details 1.A motor neuron stimulates the muscle cell by releasing the neurotransmitter acetylcholine ACh into the synaptic cleft between the neuron and muscle cell. Note: A motor unit is a single motor neuron and all the muscle fibers it activates

15. Contraction Details 1.A motor neuron stimulates the muscle cell by releasing the neurotransmitter acetylcholine ACh into the synaptic cleft between the neuron and muscle cell. 2.ACh causes an electric current called an action potential to move through the muscle cell.

16. Contraction Details 1.A motor neuron stimulates the muscle cell by releasing the neurotransmitter acetylcholine ACh into the synaptic cleft between the neuron and muscle cell. 2.ACh causes an electric current called an action potential to move through the muscle cell. 3.The action potential causes the release of Ca 2+ from the sarcoplasmic reticulum.

17. Contraction Details 4.Ca 2+ exposes myosin-binding sites on actin filaments. 5.Myosin heads (& ADP) attach to actin binding sites, forming cross-bridges. Muscle relaxed. Ca 2+ present. No Ca 2+ present. Cross-bridge formed. myosin actin ADP + P myosin head

18. Contraction Details 6.Myosin heads release ADP, move the actin filament in “power stroke” Power stroke, ADP + P released myosin actin

19. Contraction Details 6.Myosin heads release ADP, move the actin filament in “power stroke” 7.ATP binds to myosin head. The crosslink between actin and myosin breaks. 8. ATP becomes ADP + P, readying the myosin head to reattach to actin. Power stroke, ATP binds, ADP + P released cross-links break myosin actin

20. Contraction Details If Ca 2+ is still present, cycle will repeat, with myosin heads reattaching and contracting the muscle even more. If Ca 2+ is still present, cycle will repeat, with myosin heads reattaching and contracting the muscle even more. Once the actionOnce the action potential is over, the potential is over, the Ca 2+ is reabsorbed into Ca 2+ is reabsorbed into the sarcoplasmic the sarcoplasmic reticulum. Without reticulum. Without Ca 2+, myosin cannot Ca 2+, myosin cannot attach to actin. attach to actin. Watch me!

21. Contraction Details NOTE: ATP is required to break cross-links, not to form them. Explains rigor mortis Why then do muscles need ATP? To reset head so it can contract further -- contraction is a series of sliding motions.

22. Turn & Talk Describe the role of each of the following in muscle contraction Scholar with more siblings…. AChACh Ca 2+Ca 2+ Scholar with less siblings … ATPATP Action potentialAction potential

23. Exit Ticket 1.In comparing electron micrographs of a relaxed skeletal muscle fiber and a fully contracted muscle fiber, which would be seen only in the relaxed fiber? a)Z discs b)Triads c)I bands d)A bands e)H zones

24. Exit Ticket 2. Which word describes the unit of contraction of a muscle? a)Myofibril b)Sarcomere c)A band d)H band

25. Exit Ticket 3. Which of the following correctly lists the order of structure of the muscle from largest to smallest? a)fascicle, myofibril, sarcomere, muscle fiber b)myofibril, fascicle, sarcomere, muscle fiber c) fascicle, muscle fiber, myofibril, sarcomere d)muscle fiber, fascicle, myofibril, sarcomere

26. Exit Ticket 4.Which of these stores calcium ion? a)Sarcoplasmic reticulum b)Sarcomere c)Sarcolemma d)mitochondria

27. Exit Ticket 5.Which of these best describe the process of muscle contraction? a)The actin filaments shorten b)The myosin filaments shorten c)The light bands shorten d)The dark bands shorten

28. Exit Ticket 6. Which of these best describe the process of muscle contraction? muscle contraction? a)Myosin heads attach to actin filaments that are exposed by the presence of ATP b)Myosin heads attach to actin filaments that are exposed by the presence of Ca 2+ c)Actin heads attach to myosin filaments that are exposed by the presence of ATP d)Actin heads attach to myosin filaments that are exposed by the presence of Ca 2+