Presentation on theme: "The Effects of Caffeine on Plant Germination"— Presentation transcript:
1The Effects of Caffeine on Plant Germination By: Erin Chandler
2QuestionHow will different amounts of caffeine exposure effect the growth and germination of radish seeds?
3Background Information #1 drug in AmericaCaffeine is better known as trimethylxanthineThe chemical formula of trimethylxanthine is C8H10N4O2Naturally made in some plantsUsed in sportsCaffeine and the brainGrowth hormone or inhibitor?Raphannus SativusGrowing conditionsProcess of germination
4HypothesisIf seeds are exposed to caffeine solutions of various concentrations, then those exposed to the highest concentration will have higher germination rates and longer root lengths then those exposed to solutions with lower caffeine concentration.
5Materials bleach dropper distilled water gloves 5 beakers 200mg caffeine pillmortar and pestleradish seeds20 Petri dishes12 pieces of filter paper
6ProcedureWear gloves as seeds were soaked in a bleach/water solution and rinseLabel beakers 1-5 and fill beaker 1 with 100 mL of waterCrush caffeine pill and put it in beaker 2 with 100 mL of water, stirTake 10 mL from beaker 2 and add to beaker 3 along with 90 mL of water, stirTake 10 mL from beaker 3 and add it to beaker 4 along with 90 mL of water, stirTake 10 mL from beaker 4 and add it to beaker 5 along with 90 mL of water, stirLabel Petri dishes according to caffeine concentration and then trials 1-4Soak filter paper in 3 mL of solution and place in appropriate Petri dishPut 10 radish seeds in each Petri dishPut Petri dishes in a dark enclosed area for a certain amount of time
7Variables Control: seeds exposed to distilled water Independent Variable: amount of caffeine in each solutionDependent Variables: amount of seeds that germinate, and root lengthsConstants: amount of solution on filter paper, temperature, number of seeds in each Petri dish, temperature of water
10DiscussionThe data in this experiment showed that, the caffeine did cause a change in the way the seeds germinated and grewThe solution with the most caffeine had the least amount of seeds germinated and in the control the most amount of seeds germinatedThe t test showed that all data can be interpreted because all results are lower then .05, the conclusion that can be drawn according to the t test is that caffeine has a major effect on the plant’s germinationThe hypothesis was not supported by the results generated in this experimentThese results show how caffeine has a negative effect on the seeds, and that stimulants do not help the growth of plantsCaffeine is the number one drug used in America, it can be concluded that caffeine most likely does not have a positive effect on the millions of people that consume it every day
11Changes and Further Experimentation If this experiment were to be conducted again, more trials would have been very beneficialIt also would have helped to put the Petri dishes in plastic bags so that the seeds did not dry out. This would have allowed for more growing time for the seeds which would result in more data and more accurate results. Also, testing even more amounts of caffeine on the seeds may have shown more significant results so that better conclusions could have been drawnIf a person wanted to take this experiment further they could have experimented with different kinds of seeds, put the seeds in different environments or tested the seeds with other drugs similar to caffeine
12Thank YouBrian, M., & Bryant, C. W. (n.d.). How Caffeine Works. Retrieved October 6, 2010, from Discovery Health website: wellness/drugs-alcohol/caffeine1.htmlCaffeine. (n.d.). Retrieved January 30, 2011, from chemBlink website: http://www.chemblink.com/products/ htmEnblogopedia. (n.d.). What are the effects of caffeine? Retrieved September 5, 2010, from Enblogopedia website: the-effect-of-caffeine-on-plant-growth/Greenaway, T. (2001). Plant Life. Austin, TX: Raintree Steck-Vaughn Publishers.Growing radish from seed. (2000). Retrieved September 5, 2010, from GardenAction website: fruit_veg_mini_project_june_1b_radish.aspOlympic Gold? A New Effect of Caffeine Boosts Performance. (2010, June 30). Science Daily. Retrieved from 06/ htmReid, T. (n.d.). Caffeine-What's the Buzz? National Geographic. Retrieved from http://science.nationalgeographic.com/science/health-and-human-body/human-body/caffeine-buzz.html#page=8