Experimental Procedure- prepare the sample 1. Collect water samples in sterilized 25 mL glass jars 2. Add 5 mL of sampled water to bottle of nutrients and shake for 5 seconds
Materials For this experiment you would need. Incubator Petri dishes and corresponding supplies Eye droppers Water samples Rubber gloves Counting
Experimental Procedure- growing the bacteria 3. Place the sample in a Petri dish after 3 minutes the solution gels. Invert dishes 5. The Petri dishes were incubated for 3 days at 23-26 C 4. Colonies are counted and results are graphed 5 Repeat steps 2 through 4 for all samples
Incubation Samples are incubated Maintain a constant temperature of twenty-three(23) to twenty-six(26) degrees Celsius All samples were isolates to a sterile surface to control cross contamination
Average Coliform Count in 5mL sample Distilled0 Stream49.6 Spring15.6
Conclusion The data does not support my hypothesis that stream water has more E. coli than spring water. Due to the individuality of streams and springs.
Further Improvements to test streams and their corresponding feeder springs to determine the contamination levels from the source and down-stream. the source of the contamination along the waterway.
Abstract Is Spring or Stream Water more Contaminated with E. Coli? Stream water will have the higher E. coli levels than spring water. The data supports my hypothesis that stream water has more E. coli than spring water.