Presentation on theme: "The Effect of Caffeine on the Survival and Development of Mealworms By: Anna Gillin."— Presentation transcript:
The Effect of Caffeine on the Survival and Development of Mealworms By: Anna Gillin
Question How does caffeine affect the growth, survival, and development of mealworms?
Research Caffeine (C 8 H 10 N 4 O 2 ) is a drug that stimulates the nervous system It gives consumers an increase in energy and can elevate mood Caffeine is added to many drinks and foods such as chocolate, coffee, energy drinks, and many soft drinks and is heavily consumed by people today Some side effects of caffeine are headaches, anxiety, and dizziness. High levels of caffeine can be very dangerous, especially when mixed with other foods or drinks.
Research Mealworms are the larva staged of the Darkling Beatle They are part of the Tenebrio Genus and the T. molitor species The larval stage of mealworms can last from 90 up to 114 days In their larval stage, the prime occupation of mealworms is growing and eating As mealworms grow, they shed old shells and develop new ones.
Hypothesis The group of mealworms exposed to high levels of caffeine will lose more mass and have a higher death rate than the groups exposed to lower levels of caffeine.
Materials 500 milligrams caffeine 100 milligrams caffeine 100 mealworm larvae Square plastic containers Metal screens Distilled Water Wheat Bran Corn meal Shredded Newspaper Mortar and Pestle
Procedure The mealworms were divided into Group A (no caffeine), Group B (low caffeine), and Group C (high caffeine), each consisting of 3 boxes 15 mealworms were put into each box 50 mL of the solutions were mixed with 50 grams of wheat bran and cornmeal and evenly distributed among 3 containers. The following solutions were made: – Group A: 100 mL of distilled water – Group B: 100 mgs ( ½ caffeine pill) in 100 mL of distilled water – Group C: 500 mgs (2 ½ caffeine pill) in 100 mL of distilled water The mass and survivors of the mealworms were measure after a week and new bedding and solutions/food mixtures were made and distributed in the containers
Variables Independent Variable- the amount of caffeine given to each group of mealworms Dependent Variable- the effects that caffeine had on each group of mealworms and the number of mealworms still living Control- the group of mealworms (Group C) that was not exposed to caffeine and only had food moistened by water Constants- the type of container, metal screen, temperature, food, bedding in the container, and amount of mealworms in each group
ttest (Group A, Group B) 0.034 ttest (Group A, Group C) 0.035 ttest (Group B, Group C) 0.184 ttest of the Number Dead ttest of the Percent Change in Mass Ttest (Group A, Group B) 0.306 Ttest (Group A, Group C) 0.088 Ttest (Group B, Group C) 0.708 Ttest (Group A, Group B).530 Ttest (Group A, Group C).916 Ttest (Group A, Group C).297 ttest of the Percent Change in Mass/Organism
Conclusion The hypothesis stated that the mealworms exposed to higher levels of caffeine would lose more mass and have a higher death rate. The hypothesis was partially supported because caffeine had a negative effect on survival rate of mealworms, but not the masses of the survivors It was rejected that varying levels of caffeine have different effects on mealworms
Improvements The temperature should remain constant A different source of food could be used The environment should remain consistent
Further Studies Different organisms could be tested with caffeine The same experiment could be performed for the mealworms in their adult form as beetles A wider range of caffeine levels could be used to see if different amounts of caffeine have the same effects
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Works Cited Mealworm Store. (n.d.). Mealworm Facts and Information. In Mealworm Facts and Information. Retrieved 2007, from Mealworm Store website: http://mealwormstore.com/mealworm_info.php Smith Life Science. (2009, September 29). Mealworm Diagram. In Mealworms. Retrieved October 5, 2010, from Smith Life Science website: http://www.smithlifescience.com/MLMealworms.htm University of Arizona. (1997). Darkling Beetle/ Mealworm Information. Retrieved October 21, 2010, from Center for Insect Science Education Outreach website: http://insected.arizona.edu/mealinfo.htm Majithia, N. (2008). Caffeine: Understanding the World’s Most Popular Psycoactive Drug. Retrieved October 7, 2010, from Journal of Young Investigators website: http://www.jyi.org/features/ft.php?id=1327
Thank You Please feel free to ask any questions regarding my experiment.