Presentation on theme: "The Effect of Caffeine on the Survival and Development of Mealworms"— Presentation transcript:
1The Effect of Caffeine on the Survival and Development of Mealworms By: Anna GillinGood morning, my name is Anna Gillin and I am in 10th grade. The title of my project is the effect of caffeine on the survival and development of mealworms.
2QuestionHow does caffeine affect the growth, survival, and development of mealworms?The purpose of my experiment is to assess the effects of caffeine on the growth, survival, and development of mealworms.Today, caffeine is heavily consumed by people today. It is many foods and beverages consumed daily. I chose this experiment because I was interested to see the effects of varying levels of caffeine on an organism such as mealworms.
3ResearchCaffeine (C8H10N4O2) is a drug that stimulates the nervous systemIt gives consumers an increase in energy and can elevate moodCaffeine is added to many drinks and foods such as chocolate, coffee, energy drinks, and many soft drinks and is heavily consumed by people todaySome side effects of caffeine are headaches, anxiety, and dizziness. High levels of caffeine can be very dangerous, especially when mixed with other foods or drinks.
4Research Mealworms are the larva staged of the Darkling Beatle They are part of the Tenebrio Genus and the T. molitor speciesThe larval stage of mealworms can last from 90 up to 114 daysIn their larval stage, the prime occupation of mealworms is growing and eatingAs mealworms grow, they shed old shells and develop new ones.
5HypothesisThe group of mealworms exposed to high levels of caffeine will lose more mass and have a higher death rate than the groups exposed to lower levels of caffeine.The hypothesis of the experiment was that the group of mealworms exposed to high levels of caffeine will lose more mass and have a higher death rate than the groups exposed to lower levels of caffeine.
6Materials 500 milligrams caffeine 100 milligrams caffeine 100 mealworm larvaeSquare plastic containersMetal screensDistilled WaterWheat BranCorn mealShredded NewspaperMortar and PestleThe materials I used for this experiment were500 milligrams caffeine100 milligrams caffeine100 mealworm larvaeSquare plastic containersMetal screensDistilled WaterWheat BranCorn mealShredded NewspaperMortar and Pestle
7ProcedureThe mealworms were divided into Group A (no caffeine), Group B (low caffeine), and Group C (high caffeine), each consisting of 3 boxes15 mealworms were put into each box50 mL of the solutions were mixed with 50 grams of wheat bran and cornmeal and evenly distributed among 3 containers. The following solutions were made:Group A: 100 mL of distilled waterGroup B: 100 mgs ( ½ caffeine pill) in 100 mL of distilled waterGroup C: 500 mgs (2 ½ caffeine pill) in 100 mL of distilled waterThe mass and survivors of the mealworms were measure after a week and new bedding and solutions/food mixtures were made and distributed in the containers
8VariablesIndependent Variable- the amount of caffeine given to each group of mealwormsDependent Variable- the effects that caffeine had on each group of mealworms and the number of mealworms still livingControl- the group of mealworms (Group C) that was not exposed to caffeine and only had food moistened by waterConstants- the type of container, metal screen, temperature, food, bedding in the container, and amount of mealworms in each groupI bolded the variables
14ttest of the Number Dead ttest of the Percent Change in Mass Datattest of the Number Deadttest (Group A, Group B)0.034ttest (Group A, Group C)0.035ttest (Group B, Group C)0.184ttest of the Percent Change in MassTtest (Group A, Group B)0.306Ttest (Group A, Group C)0.088Ttest (Group B, Group C)0.708I filled in the tests that showed significant differencesttest of the Percent Change in Mass/OrganismTtest (Group A, Group B).530Ttest (Group A, Group C).916.297
15ConclusionThe hypothesis stated that the mealworms exposed to higher levels of caffeine would lose more mass and have a higher death rate.The hypothesis was partially supported because caffeine had a negative effect on survival rate of mealworms, but not the masses of the survivorsIt was rejected that varying levels of caffeine have different effects on mealwormsI changed the second bullet.
16Improvements The temperature should remain constant A different source of food could be usedThe environment should remain consistentThe mealworms were kept in a basement where the temperature fluctuated which may have slowed pupationA different source of food should be used. The food was wheat bran and corn meal which was the same as the bedding. So, some of the mealworms may no have consumed caffeineThe environment should also remain the same. The mealworms were moved from school to a home environment.
17Further Studies Different organisms could be tested with caffeine The same experiment could be performed for the mealworms in their adult form as beetlesA wider range of caffeine levels could be used to see if different amounts of caffeine have the same effectsDifferent organisms could be tested. The results could be compared with the results of the mealwormsThe same experiment could be performed for the mealworms in their adult form as beetles. It would be interesting to see how the results differ.A wider range of caffeine levels could be used to see if different amounts of caffeine have the same effectsAlso, the mealworms could be fed more frequently, rather than once a week
18Works CitedGavin, M. L., & Black, J. D. (2008). Caffeine. Retrieved October 19, 2010, from The Nemours Foundation website:Kovacs, B., & Stoppler, M. C. (n.d.). Caffeine. In Caffeine [Caffeine, Health Effects, Statistics]. Retrieved October 5, 2010, from MedicineNet website:Lieberman, H. R., Tharion, W. J., Shukitt-Hale, B., Speckman, K. L., & Tulley, R. (n.d.). Biomedical and Life Sciences. In Effects of Caffeine on Sleep loss, and Stress on Cognitive Performance and Mood During U.S. Navy SEAL Training [Caffeine, Effects ]. Retrieved October 5, 2010, from SpringerLink website:
19Works CitedMealworm Store. (n.d.). Mealworm Facts and Information. In Mealworm Facts and Information. Retrieved 2007, from Mealworm Store website:Smith Life Science. (2009, September 29). Mealworm Diagram. In Mealworms. Retrieved October 5, 2010, from Smith Life Science website:University of Arizona. (1997). Darkling Beetle/ Mealworm Information . Retrieved October 21, 2010, from Center for Insect Science Education Outreach website:Majithia, N. (2008). Caffeine: Understanding the World’s Most Popular Psycoactive Drug. Retrieved October 7, 2010, from Journal of Young Investigators website:
20Thank YouPlease feel free to ask any questions regarding my experiment.Thank you for listening. I hope you found my presentation interesting and informative. Are there any questions?