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C. elegans was the first animal to have its genome (10 8 bp) completely sequenced.
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C. elegans Genome in Numbers Size (Mb) Protein genes Protein genes/kb Coding Regions (%) % of genes EST matches (Mb) 95.53 19,141 4.9827 38.9 The completed genome sequence is made up of 2,527 cosmids 113 fosmids 257 YACs 44 long range PCR products intergenic DNA 47% exonic DNA 27% intronic DNA 26% http://nema.cap.ed.ac.uk/Caenorhabditis/C_elegans.html
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Characteristics of the Caenorhabditis elegans genome Seq GenesEST’s Lev Inv. Rep. Tand Rep Telom The C. elegans genome consortium, TIG 15:51, 1999
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The study of many aspects of Nematode biology were made easier after the genome of C. elegans was sequenced After doing “discovery science” (L. Hood, 2002; “defining the elements in biological objects”) we can do regular “hypothesis-driven” science.
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Outron Transplicing and operons in C. elegans Blumenthal, T. (1998) - BioEssays 20:480-487.
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Regulatory micro RNAs (miRNAs)
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Banerjee & Slack BioEssays, 24:119, 2002 Involvement of small RNAs in the control of Temporal development and RNAi in C. elegans
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24h 48h 72h 96h Bacteria with no plasmid Kamath e cols. (2001) Genome Biol. 2:1-10 Feeding C. elegans with dsRNA turns off specific genes
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Other nematodes help define what is common to all species and what is restricted to C. elegans at the molecular level
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Made by R. Turner Department of Biology, 1990 - Indiana University CEW1C. elegans Anterior Left side SEM of CEW1 and C. elegans
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Although the final morphology is the same, the induction events during vulva development are difrerent in C. elegans and CEW1 Dichtel et al., Genetics, 157:183, 2001
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CEW1 Transplicing in C. elegans and CEW1 From Evans et al., PNAS 94:9751, 1997
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Introns of the C. elegans vitellogenin genes Winter, in: “Reproductive Biology of Invertebrates,Volume XII” - 2002
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NJ-Tree of nematode vitellogenins (aminoacid sequences) Winter, in: “Reproductive Biology of Invertebrates,Volume XII” - 2002
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N- and C-terminus of nematode VTG evolve at different rates Winter et al., Mol. Biol. Evol., 13:674, 1996
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CUU CUC CUA CUG 51 24 39 55 1 2 0 12 CCA CCC 1 99 64 9 85 17 15 83 CAA CAG GAU GAC 50 23 77 Ce CEW1 Ce CEW1 Ce CEW1 Codon usage table of vit-6 genes in C. elegans and CEW1 Winter et al., Mol. Biol. Evol., 13:674, 1996
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Acknowledgements Dr Cristiane Penha-Scarabotto Dr Rubens Nobumoto Akamine Joselene Pereira de Moura Il-Young Ahn Juliana Machado Andreoni Paulo Afonso de Carvalho Daniela Peres Almenara Manoel Aparecido Peres SUPPORTED BY & Undergraduate students
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“... We found that bringing together the true cross- disciplinary scientists was rendered difficult by the fact that our academic center, and most academic centers, live in a world of departments. And the departments tend to create barriers both in how their students are educated and what the expectations are for faculty...” Leroy Hood, JAN/18/2002 [see http://www.oreillynet.com/pub/a/network/2002/01/18/hood.html] http://www.oreillynet.com/biocon2002/?xA;
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Wormbase How are the genome sequences of C. elegans stored Published in: http://nema.cap.ed.ac.uk/Caenorhabditis/C_elegans_genome/Celegansinformatics.html
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Distribution of repetitive elements on the C. elegans chromosomes Surzycki and William R. Belknap, PNAS 97:245–249, 2000 Cele 14 Cele 2 Cele 1 MITE-like Cele 42
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Saigusa et al., Curr. Biol. 12(2):R46, 2002 Movement Kippert et al., Curr. Biol. 12(2):R47, 2002 Osmotic Shock Resistance C. elegans behaviour is subjected to circadian variations
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Caenorhabditis elegans vitellogenin genes geneintron nr. location DNA strand "YP" Size of the vitellogenin (kDa)(*) cDNA clones (**) chromosome: position vit-14K09F5.2+170B186.617X: 7,742,201- 7,746,243 vit-2 4C42D8.2+170B186.2118X: 5,114,376- 5,119,411 vit-35F59D8.1-170A185.01X: 3,626,224- 3,612,142 vit-4 5F59D8.2-170A184.85X: 3,618,023- 3,612,577 vit-55C04F6.1+170A184.969X: 3,416,833- 3,421,907 vit-64K07H8.6+115/ 88 191.0126IV: 8,273,968- 8,279,162 * = calculated from the amino acid sequence deduced from the gene sequence; signal peptide excluded. ** = Number of cDNA clones listed in WormBase (http://www.wormbase.org/) (Jan/30/2001) associated with each gene Winter, in: “Reproductive Biology of Invertebrates, Volume XII” - 2002
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Embriogenesis of Acrobeloides nanus Schierenberg (1999) - http://www.uni-koeln.de/math-nat-fak/zoologie/agschier/abschier3e.html
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of man Modified from: “Topics in Intern. Health – Schistosomiasis” The Wellcome Trust
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