1. Extracting Chlorophyll Using paper chromatography
The chemical formula for water =
H2O
Hydrogen = H
H2 = 2 atoms of Hydrogen
Oxygen = O
H2O

2. Extracting Chlorophyll Using paper chromatography
The chemical formula for carbon dioxide =
CO2
Carbon = C
Oxygen = O
O2 = 2 atoms of Oxygen
CO2

3. Extracting Chlorophyll Using paper chromatography
Scientists write equations to show chemical reactions
The formation of water from hydrogen and oxygen is written as:
2H2 + O2
2H2O + energy

4. Extracting Chlorophyll Using paper chromatography
Scientists write equations to show chemical reactions
2H2 + O2
2H2O + energy
Two molecules of water are formed
Energy is released

5. Extracting Chlorophyll Using paper chromatography
The most important chemical reaction in the world is…
PHOTOSYNTHESIS
The chemical reaction for photosynthesis is
6CO2 + 6H2O + energy C6H12O6 + 6O2

6. Extracting Chlorophyll Using paper chromatography
PHOTOSYNTHESIS
Sugar
Carbon Dioxide
6CO2 + 6H2O + energy C6H12O6 + 6O2
Oxygen
Water
Sunlight

7. Extracting Chlorophyll Using paper chromatography
PHOTOSYNTHESIS
Plants possess a light trapping molecule called
Chlorophyll

8. Extracting Chlorophyll Using paper chromatography
PHOTOSYNTHESIS
Chlorophyll
Captures light energy and uses it to make food
Gives leaves their green color

9. Extracting Chlorophyll Using paper chromatography
PHOTOSYNTHESIS
Chlorophyll
Gives leaves their green color
Comes in many colors and two forms
Chlorophyll a
Chlorophyll b

10. Extracting Chlorophyll Using paper chromatography
PHOTOSYNTHESIS
Chlorophyll
Chlorophyll a
Chlorophyll b
Both forms absorb different wavelengths of light

11. Extracting Chlorophyll Using paper chromatography
PHOTOSYNTHESIS
Chlorophyll
In this paper chromatography experiment
Will appear as a bright yellow-green band
Chlorophyll a
Will appear as a dull green band
Chlorophyll b

12. Extracting Chlorophyll Using paper chromatography
Accessory Pigments that help transfer light to the chlorophyll
Will appear as an orange band
Carotene
Will appear as a yellow band
Xanthophyll
Anthocyanin
(acts as plant sunscreen)
Will appear as a red-brown band

13. Extracting Chlorophyll Using paper chromatography
Materials
Filter paper cut into strips
Pennies
Straw or stirrer
Beaker
Rubbing alcohol
Tape
Ruler
Plant leaves

14. Extracting Chlorophyll Using paper chromatography
Procedure
Draw a line across the paper strip, 2 cm from one end
Place the leaf you want to test on top of the filter paper strip, remembering where the line is

15. Extracting Chlorophyll Using paper chromatography
Procedure
Using the edge of a penny, trace the penny across the leaf right on top of where you drew the line
Push down hard enough to leave a green smear on the paper on top of the pencil mark

16. Extracting Chlorophyll Using paper chromatography
Procedure
Repeat 2 more times with fresh sections of leaf to make the smear darker
Try to keep the smear as close to the original pencil line as possible

17. Extracting Chlorophyll Using paper chromatography
Procedure
Fill the beaker to a depth of 1 cm with rubbing alcohol
Carefully lower the filter paper strip, green smear end in first, into the beaker until the bottom just touches the alcohol

18. Extracting Chlorophyll Using paper chromatography
Procedure
Make sure that the green smear does not actually touch the alcohol, only the tip of the paper
Lay the stick across the mouth of the beaker like a bridge from one edge to the other

19. Extracting Chlorophyll Using paper chromatography
Procedure
Tape the filter paper strip to the stick so that the paper is held in place just touching the alcohol but not touching the sides of the beaker

20. Extracting Chlorophyll Using paper chromatography

21. Extracting Chlorophyll Using paper chromatography
Procedure
Wait. The alcohol should gradually move up the paper, bringing many of the pigments along with it
Remove the stick and paper when the alcohol has almost reached the stick

22. Extracting Chlorophyll Using paper chromatography
Procedure
Mark how far up the paper the alcohol traveled with a pencil
Determine how many bands of pigment you have, what color they are, and measure how far each band traveled from the origin line

23. Extracting Chlorophyll Using paper chromatography
Procedure
Identify the pigments you found based on their colors
Draw a color chart of what you found
Prepare a report out for the class