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Suppl. Fig.1 46 58 80 30 - + + TET -  -Flag  -Tub Flag-TDP-43WT Flag-TDP-12xQ/N.

Published byMercedes Yonge Modified over 9 years ago

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Presentation on theme: "Suppl. Fig.1 46 58 80 30 - + + TET -  -Flag  -Tub Flag-TDP-43WT Flag-TDP-12xQ/N."— Presentation transcript:

1 Suppl. Fig.1 46 58 80 30 - + + TET -  -Flag  -Tub Flag-TDP-43WT Flag-TDP-12xQ/N

2 Suppl.Fig. 2  - TDP-43  -HSP70 MERGE (  - TDP-43/  -Flag/TOPRO-3 MERGE (  - TDP-43/  -HSP70 Flag-TDP-43WT (+ TET) Flag-TDP-12xQ/N (+ TET)

3  - HSP70 83 47.5  - TDP-43 beads Flag-beads beads Flag-beads Flag- TDP-43WT Flag- TDP-12xQ/N Flag- TDP-43WT Flag- TDP-12xQ/N IP Inputs HSP70 Flag-TDP-12xQ/N Flag-TDP-43 endo-TDP-43 Suppl.Fig.3

4 47.5 83  - Flag  - Actin 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 TDP-12xQ/N F4L TDP-12xQ/N TDP-43WT - + TET - + - + - + - + TDP-12xQ/N  RRM1 - + TDP-12xQ/N  RRM2 - + - + TDP-12xQ/N  RRM1/2 TDP-12xQ/N  RRM1/2,  N TDP-12xQ/N  RRM1/2,  C - + TDP-12xQ/N  RRM1 TDP-12xQ/N  RRM2 - + Suppl. Fig.4 TDP-12xQ/N  RRM1 TDP-12xQ/N  RRM2 A B  - POLDIP3 TET E2 (variant 1) E3E4 E2E4 (variant 2) (variant 1) (variant 2)

5 Suppl. Fig.5 TDP-12xQ/N  N TDP-12xQ/N TDP-43WT - + TET - + + C A B RT-PCR E2E4 E2E3E4 (variant 1) (variant 2) - TDP-12xQ/N RRM1RRM2 NLSNESQ/N NES RRM1RRM2 TDP-12xQ/N  N TDP-12xQ/N TET + +  -FLAG 80  -POLDIP3 variant 1 variant 2 47.5 TDP-12xQ/N  N TDP-12xQ/N TET + +

6 Flag- TDP-12xQ/N Flag-TDP-12xQ/N  C Flag- TDP-12xQ/N Flag-TDP-12xQ/N  C Flag- TDP-12xQ/N Flag-TDP-12xQ/N  C Beads IP:  - Flag Inputs WB:  - Flag Flag- TDP-12xQ/N Flag-TDP-12xQ/N  1/2  C WB:  - TDP-43 endo-TDP-43 1 2 3 4 5 6 Suppl. Fig.6

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