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A 198 115 90.5 123 IP: HA WCE +++ Hdm2 HA-Ub FLAG-hTERT MG132 - ++ +++ -- + WB: FLAG WB: Hdm2 WB: FLAG hTERT -(Ub)n Supplementary Figure S1. Oh et al.

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Presentation on theme: "A 198 115 90.5 123 IP: HA WCE +++ Hdm2 HA-Ub FLAG-hTERT MG132 - ++ +++ -- + WB: FLAG WB: Hdm2 WB: FLAG hTERT -(Ub)n Supplementary Figure S1. Oh et al."— Presentation transcript:

1 a IP: HA WCE +++ Hdm2 HA-Ub FLAG-hTERT MG WB: FLAG WB: Hdm2 WB: FLAG hTERT -(Ub)n Supplementary Figure S1. Oh et al. WB: HA WB: Hdm2 WB: FLAG WCE MG132 Hdm2 HA-Ub FLAG-hTERT hTERT -(Ub)n WB: FLAG IP: FLAG b

2 a IP: FLAG WCE hTERT -(Ub)n WT Ring ++++ Hdm2 HA-Ub FLAG-hTERT MG WB: HA WB: Hdm2 WB: FLAG d IP: FLAG WCE hTERT -(Ub)n WB: HA WB: Hdm2 WB: FLAG WT C464A ++++ Hdm2 HA-Ub FLAG-hTERT MG WB: Hdm2 WB: HA WCEIP: HA 1234 Hdm2 C464A hTERT-HA wt C464A WB: Hdm2 WB: HA WB: GFP Hdm2 C464A hTERT-HA b c Supplementary Figure S2. Oh et al.

3 Supplementary Figure S3. Oh et al. hTERT -(Ub)n WCE PD: Ni-NTA WB: Myc WB: HA Myc-Hdm2s His-Ub hTERT-HA MG WT WT a Myc-Hdm PC NC wt hTERT IC (36bp) 50 bp b

4 a hTERT RT b Supplementary Figure S4. Oh et al. hTERT-HA Hdm hTERT IP: Hdm2 WCE Hdm2 hTERT

5 a cb SC-7 PC SC-10 Mock1 d NC e Mock2 WB: FLAG WB: HA 1234 FLAG-Hdm2 hTERT-HA WCE IP: FLAG FLAG-Hdm2 hTERT-HA GFP FLAG-Hdm2 hTERT-HA GFP kDa 90.5 WB: HA WB: Hdm2 WB: Myc WCE 123 hTERT -(Ub)n WB: Myc IP: Myc +++ FLAG-Hdm2 HA-Ub Myc-hTERT MG Mock-1 SC-7 SC-10 Mock-2 FLAG-Hdm2 Actin IC (36bp) 50 bp Supplementary Figure S5. Oh et al.

6 a cb Supplementary Figure S6. Oh et al. R1 FLAG-negative R2 FLAG-positive R1 FLAG-negative R2 FLAG-positive R1 HA-negative R2 HA-positive


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