1. Apaf-1 Caspase-9 Cyt c control sulindac control E LY294002 control TRAIL thapsigargin HT-29Chondrocyte control SIN-1 RAW 264.7 IP: AChE C Supplementary Fig. S1 AB IP : Apaf-1 WB: AChE IP: Caspase-3 WB: AChE IP: Caspase-9 WB: AChE IP: c-IAP-1 WB: AChE IP: c-IAP-2 WB: AChE IP: Cyt c WB: AChE E40 - + treated Thapsigargin 2.5  M, 24 h HT-29 Sulindac 1 mM, 48 h E40 40 h LY294002 100  M, 24 h TE671 BMMC U373MG E50 24 h E40 24 h SK-MEL5 E10 48 h RAW 264.7 SIN-1 1 mM, 24 h IL-3 deprivation Malme-3M E50 24 h TRAIL 100 MOI, 72 h Chondrocyte SNP 1.5 mM, 24 h  -actin control treated

2. E40 si Cyt c Cyt c Hoechst ---- +-+- -+-+ ++++ A Supplementary Fig. S2 B si Cyt c 0 8 16 24 48 h Chondrocyte TRAIL 100 MOI HT-29 Thapsigargin 2.5  M Apaf-1 IP: AChE IgG 105 50 105 50 IP: Apaf-1 WB: Apaf-1 IP: Apaf-1 WB: Apaf-1 IgG PC IP: AChE SK-MEL-5 E10 RAW 264.7 SIN-1 1 mM 0 8 16 24 48 h 105 50 105 50 Apaf-1 IgG si Cyt c IP: Apaf-1 WB: Apaf-1 IP: Apaf-1 WB: Apaf-1 PC

3. Supplementary Fig. S3 IP : Cav-1 E40 0 8 16 24 48 72 h AChE IgG 50 80 A B si Cyt c E40 0 8 16 24 48 72 h IP: Cav-1 IgG AChE 50 80 C D IP: Apaf-1 WB: AChE E50 (LNCaP)E10 (PC-3) 0 24 48 h IP: Cav-1 WB: AChE IP: Cyt c WB: AChE IP: Cyt c WB: Apaf-1 IP: Apaf-1 WB: AChE IP: Cav-1 WB: AChE IP: Cyt c WB: AChE IP: Cyt c WB: Apaf-1 IP: AChE WB: AChE IP: AChE WB: AChE IP: AChE WB: AChE IP: Apaf-1 WB: Apaf-1 IP: AChE WB: AChE IP: AChE WB: AChE IP: AChE WB: AChE IP: Apaf-1 WB: Apaf-1 PC IgG E40 0 8 16 24 48 h 105 50 15 IgG Apaf-1 Cyt c IP: Cav-1 IP: Apaf-1 WB: Apaf-1 IP: Cyt c WB: Cyt c IgG PC E IP: Cav-1 M  CD E40 -+-+ 1/12 + 1+1+ 2+2+ 24h + ---- AChE IP: Apaf-1 AChE Cyt c IgG 50 25 IP: Cyt c 50 80 50 80