2. Immunology (elective) MLIM-101 Prepared by: Dr. Mohamed S. Abdel-Latif

3. Complements

4. Learning Outcome: At this time you should know the following: 1. Proteins of the complement system. 2. Differences and similarities among the different pathways of C3 activation. 3. Significance of the different pathways in specific and nonspecific immunity. 4. Role of different complement activation products in amplification of nonspecific and specific immunity and inflammation.

5. Complement: history Discovered in 1894 by Bordet It represents lytic activity of fresh serum Its lytic activity destroyed when heated at 56C for 30 min

6. Complement functions Host benefit: opsonization to enhance phagocytosis phagocyte attraction and activation lysis of bacteria and infected cells regulation of antibody responses clearance of immune complexes clearance of apoptic cells Host detriment: Inflammation, anaphylaxis

7. C-activation: alteration of C proteins such that they interact with the next component C-fixation: utilization of C by Ag-Ab complexes Hemolytic units (CH50) : dilution of serum which lyses 50% of Ab-coated r.b.c in a suspension C-inactivation: denaturation (usually by heat) of an early C-component resulting in loss of hemolytic activity Convertase/esterase: altered C-protein which acts as a proteolytic enzyme for another C-component Definitions

8. Proteins of the complement system (nomenclature) C1(qrs), C2, C3, C4, C5, C6, C7, C8, C9 factors B, D, H and I, properdin (P) mannose binding lectin (MBL), MBL associated serine proteases (MASP-1 MASP-2) C1 inhibitor (C1-INH, serpin), C4-binding protein (C4-BP), decay accelerating factor (DAF), C1 receptor (CR1), protein-S (vitronectin)

9. Activation product of complement proteins (nomenclature) When enzymatically cleaved, the larger moiety, binds to the activation complex or membrane and the smaller peptide is released in the microenvironment Letter “b” is usually added to the larger, membrane-binding, peptide and “a” to the smaller peptide (e.g., C3b/C3a, C4b/C4a, C5b/C5a), EXCEPT C2 (the larger, membrane- binding moiety is C2a; the smaller on is C2b) Activated component are usually over-lined: e.g. C1qrs

10. Pathways of complement activation CLASSICAL PATHWAY ALTERNATIVE PATHWAY activation of C5 LYTIC ATTACK PATHWAY antibody dependent LECTIN PATHWAY antibody independent Activation of C3 and generation of C5 convertase

11. Components of the Classical Pathway C4 C2 C3 C1 complex Ca ++ C1r C1s C1q

12. Ca ++ C1r C1s C1q C4 C4a b Classical Pathway Generation of C3-convertase

13. C4b Mg ++ C4a Ca ++ C1r C1s C1q C2 C2b a C2 a C4b2a is C3 convertase

14. Classical Pathway Generation of C5-convertase C4b Mg ++ C4a Ca ++ C1r C1s C1q C2b C2 a C3 C3a b C4b2a3b is C5 convertase; it leads into the Membrane Attack Pathway

15. Components of mannose-binding lectin pathway C4 MBL C2 MASP1 MASP2

16. Mannose-binding lectin pathway C4 MBL C4b C4a C4b C2 C2b C2a C4b2a is C3 convertase; it will lead to the generation of C5 convertase MASP1 MASP2

17. Components of the alternative pathway C3 B D P

18. Spontaneous C3 activation C3 H2OH2O i B D Generation of C3 convertase C3iBb complex has a very short half life b C3 C3a b

19. B D b C3b If spontaneously-generated C3b is not degraded C3-activation the amplification loop C3 C3a b

20. B D BbBb C3b C3 b C3-activation the amplification loop C3b C3a b

21. BbBb C3b C3 BbBb B D b b C3a C3-activation the amplification loop C3b

22. C3a BbBb C3b BbBb BbBb C3a C3-activation the amplification loop C3b

23. C3a BbBb C3b BbBb BbBb C3a C3-activation the amplification loop C3b

24. Control of spontaneous C3 activation via DAF C3b DAF prevents the binding of factor B to C3b B Autologous cell membrane DAF CR1

25. Control of spontaneous C3 activation via DAF DAF dislodges C3b-bound factor Bb B bb C3b Autologous cell membrane DAF CR1 B b

26. Autologous cell membrane C3b B b H I iC3b Control of spontaneous C3 activation via CR1 B b I iC3b DAF CR1 DAF CR1

27. Degradation of spontaneously produced C3b C3b iC3b II C3dg C3c

28. C3b stabilization and C5 activation C3b C3b finds an activator (protector) membrane C3 C3a b B D b P This is stable C5 convertase of the alternative pathway

29. C3b regulation on self and activator surfaces C3b

30. C5-convertase of the two pathways C3b BbBb C5-convertase of the Alternative Pathway C4b C2a C3b C5-convertase of the Classical and lectin Pathways

31. Generation of C5 convertase leads to the activation of the Lytic pathway

32. Components of the lytic pathway C6 C9C9 C8 C7 C5

33. Lytic pathway C5-activation C3b C2 a C4b C5 b C5a

34. Lytic pathway assembly of the lytic complex C5 b C6 C7

35. Lytic pathway: insertion of lytic complex into cell membrane C5 b C6 C7 C8 C9C9 C9C9 C9C9 C9C9 C9C9 C9C9 C9C9 C9C9 C9C9

36. C 1 I nh C1qrs breakdown C1r C1s C1q C1r C1s

37. C1-inhibitor deficiency: angioedema

38. Biological effects of C5a

39. Opsonization and phagocytosis

40. ProductBiological EffectsRegulation Biological properties of C-activation products C2b (prokinin) edemaC1-INH C3a (anaphylatoxin) mast cell degranulation; enhanced vascular permeability; anaphylaxis carboxy- peptidase- B (C3-INA)

41. ProductBiological EffectsRegulation Biological properties of C-activation products as C3, but less potent (C3-INA) C4a (anaphylatoxin) opsonization; phagocytosis C4b (opsonin) C4-BP, factor I C3b (opsonin) opsonization; phagocyte activation factors H & I

42. ProductBiological EffectsRegulation Biological properties of C-activation products anaphylactic as C3, but much more potent; attracts & activates PMN causes neutrophil aggregation, stimulation of oxidative metabolism and leukotriene release C5a (chemotactic factor) carboxy- peptidase-C (C3-INA) C5b67protein-Schemotaxis, attaches to other membranes

43. Assignment: As a part of the semester activity, group of students are selected every week to prepare a short seminar about his/her point of interest in one of the lecture topics. That to be discussed and evaluated during the next lecture.