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Nanopore Membranes for Single-Molecule Detection and Characterization Anna Schibel, Ryuji Kawano and Henry S. White University of Utah October 26, 2007.

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Presentation on theme: "Nanopore Membranes for Single-Molecule Detection and Characterization Anna Schibel, Ryuji Kawano and Henry S. White University of Utah October 26, 2007."— Presentation transcript:

1 Nanopore Membranes for Single-Molecule Detection and Characterization Anna Schibel, Ryuji Kawano and Henry S. White University of Utah October 26, 2007

2 The Glass Nanopore (GNP) Membrane Zhang et al. Anal. Chem. 2007, 79,

3 Fabrication of the GNP Membrane Zhang, B.; Galusha, J.; Wang, G.; Bergren, A. J.; Jones, R. M.; White, R. J.; Ervin, E. N.; Cauley, C. C.; Shiozawa, P.; White, H. S. Anal. Chem. 2007, 79, Bo Zhang Nanodisk electrodeNanopore electrodeNanopore membrane

4 Stochastic Single-Molecule Detection - hepta-sulfano-  -cyclodextrin (s 7  CD) R = -OSO 3 - Bound State Unbound State -  -Hemolysin (  HL)  HL insertion Single Binding Events Applied Voltage -40 mV Gu et al., Science 2001, 291, M KCl 10 mM Potassium phosphate buffer pH  M aHL backside 50  M s 7  CD frontside 250 nm pore

5 Stochastic Single-Molecule Detection R = NH 3 + Clement Osei Akoto Rainer Group trans cis - Reference + - a 7  CD  M a 7  CD Voltage in wrt cis side 50  M a 7  CD detection Electrostatic trapping Electrostatically trap molecule inside  HL channel 1 s i a 7  CD GNP radius - 95 nm

6 Nanoparticle Detection Wang, G., Single Nanoparticle Sensing with Protein Ion Channel Embedded in Lipid Bilayer Supported By Glass Nanopore Membrane (unpublished) Gold Nanoparticle with Tiopronin Monolayer 2nm

7 Characterization of Hairpin DNA Structures 9bp-GC9bp-AT9bp-TG Common structure

8 1 sec 9 bp-GC 1 sec 9 bp-TG 1 sec 9 bp-AT ~1 minute Current Discrimination Of Individual DNA Hairpin Molecules

9 Heparin Detection Heparin

10 Applications In Development Biomedical Analysis DNA sequencing Heparin analysis Homeland Defense Chemical warfare agent detection Environmental Analysis Ultra-trace level detection of pollutants Nano-particle analysis

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