1. AGH University of Science and Technology
Comparative analysis of aberration yields induced by radiation with different LET (Linear Energy Transfer)
University of Warsaw
Jakub Pietrzak UW
University of Gdańsk
Magdalena Rojek
Monika Szymanska
AGH University of Science
and Technology
Anna Kaminska
Dominika Kedzierska
Ewa Lagudza

2. THE DREAM TEAM

3. Laboratory of Radiation Biology
Marta Deperas- Kaminska (supervisor)
Ekaterina Zaytseva

4. The practice purposes:
Theoretical introduction to cytogenetic methods used in biodosimetry
Instructions of safety rules of work with human blood samples.
To learn how to irradiate human lymphocytes and culture them under experimental conditions.
Be able to carry out a simple experiment and analysis with irradiated cells

5. Standard light microscope
SCHEME OF THE EXPERIMENT
Incubation for the standard metaphase analysis
colcemid
47h
Fixation
Staining with Giemsa
50h
Standard light microscope
Radiation

6. Our experiment
Irradiation of blood samples with different LET radiation
Lymphocytes culture (48 hours)
Incubation for standard metaphase analysis
Picture 2 Lymphocyte
Picture 1 Blood

7. Analysis of chromosomes aberrations
Lymphocytes fixation
Giemza staining
Analysis of chromosomes aberrations
Picture 3 Chromosomes
Picture 4 Chromosomes 2

8. Unstable aberrations - dicentrics, acentric fragments and centric and acentric rings in Giemsa stained metaphase
Dic
Centric ring
Acentric ring
deletion
ace

9. RESULTS:
The increase of aberration yields with increase of radiation LET in human peripheral blood lymphocytes is observed.

10. Acknowledgements Special thanks to: Dmitri Vladimirovich Fursaev
Wladyslaw Chmielowski, Roman Zawodny
Marta Deperas-Kaminska, Katia Zaytseva

11. THANKS FOR YOUR ATTENTION