Presentation is loading. Please wait.

Presentation is loading. Please wait.

Aulani "Biokimia Enzim" Presentasi 10 Enzyme Phosphorylation Aulanni’am Biochemistry Laboratory Chemistry Department Brawijaya University.

Similar presentations


Presentation on theme: "Aulani "Biokimia Enzim" Presentasi 10 Enzyme Phosphorylation Aulanni’am Biochemistry Laboratory Chemistry Department Brawijaya University."— Presentation transcript:

1 Aulani "Biokimia Enzim" Presentasi 10 Enzyme Phosphorylation Aulanni’am Biochemistry Laboratory Chemistry Department Brawijaya University

2 Aulani "Biokimia Enzim" Presentasi 10 Phosphorylation Conformational Change dephosphorylastion Phosphatase P Protein OH Ser Ser Thr Tyr (His) A ctive Inactive Inactive Active Glycogen phosphorylase bGlycogen phosphorylase a Fischer, Kreb (1978) Kinase phosphorylation

3 Aulani "Biokimia Enzim" Presentasi 10 Regulation of Blood Sugar High blood sugar Insulin Pancreas Glycogen Glucose Decrease Glycogen synthase Increase Hormone Signal Transduction Blood Liver Low blood sugar Glucagon GTP-protein-linked receptor Tyrosine-kinase-linked receptor Glycogen phosphorylase Cori & Cori (1947)

4 Aulani "Biokimia Enzim" Presentasi 10 Rapid Response to Blood Sugar Concentration Glycogen phosphorylaseGlycogen synthase Enzyme activity Time (min) Very rapidly → Not controlled in gene level

5 Aulani "Biokimia Enzim" Presentasi 10 Glycogen Phosphorylase, GP Glycogen n n -1 Glc-1-P → Glc-6-P → Glycolysis Glycogen phosphorylase a* Phosphatase GP kinase Glycogen phosphorylase b (inactive) ATP Protein kinase A cAMP Phosphorylase + + AMP (+) ATP (-) Glc-6-P (-) Glucose (-) Caffeine (-) P RTRT PP Juang RH (2004) BCbasics

6 Aulani "Biokimia Enzim" Presentasi 10 cAMP Controls Protein Kinase A Activity R C R C R R A A A A A A A A C C Regulatory subunits Catalytic subunits cAMP Active kinase C CREB P Nucleus Activation Gene expression DNA Alberts et al (2002) Molecular Biology of the Cell (4e) p. 857, 858

7 Aulani "Biokimia Enzim" Presentasi 10 G protein cAMP Is the Second Messenger Glycogen Glc-1-P P GP1 a GP b P GP Kinase Protein Kinase A cAMP ATP Cyclase G protein Adenylate cyclase A Cyclic AMP (second messenger) GP kinase GP Receptor Glucagon Sutherland (1971) Cascade Inactive Active Juang RH (2004) BCbasics

8 Aulani "Biokimia Enzim" Presentasi 10 SH2 domain Receptors on Cell Membrane b G protein a g GDP b g + Signal -GDP +GTP a GDP a GTP a Adenylate cyclase + Signal b Activation P Protein Phosphatase a Glycogen Synthase Glycogen Synthase P active Insulin PP PP kinase Glucagon A G-protein-linked Receptor Enzyme-linked Receptor The third group: Ion-channel-linked Receptor Gilman, Rodbell (1994) Glycogen breadkdown Glycogen synthesis Juang RH (2004) BCbasics

9 Aulani "Biokimia Enzim" Presentasi 10 PP A GP kinase GP a GP b Glycogen synthase P Protein phosphatase-1 Protein phosphatase inhibitor-1 Glycogen PKA P active inactive Phosphatase Glucagon Adapted from Kleinsmith & Kish (1995) Principles of Cell and Molecular Biology (2e) p.217

10 Aulani "Biokimia Enzim" Presentasi 10 Signal Transduction Receptor Hormone Signal G Cyclase Transducer Effector Enzyme Effector Effect 各種荷爾蒙 各種接受體 不同的 G-protein 各種關鍵酵素 各種信息分子 各種效應 Juang RH (2004) BCbasics

11 Aulani "Biokimia Enzim" Presentasi 10


Download ppt "Aulani "Biokimia Enzim" Presentasi 10 Enzyme Phosphorylation Aulanni’am Biochemistry Laboratory Chemistry Department Brawijaya University."

Similar presentations


Ads by Google