1. I Zapolsky, E Kyzar, S Gaikwad, A Roth, J Green, M Pham, PG St. Pierre, B Hirons, AV Kalueff (New Orleans, USA)

2. ZEBRAFISH NEUROPHENOME PROJECT The ZNP was started by the ZNRC, and is dedicated toward producing a ZND The ZNP was started by the ZNRC, and is dedicated toward producing a ZND Let’s start by defining these acronyms Let’s start by defining these acronyms

3. The ZNRC Zebrafish Neuroscience Research Consortium Allan Kalueff - USA Georgianna Gould - USA Oliver Braubach - Canada Anderson Manoel Herculano - Brazil David Echevarria - USA Joe Schroeder - USA Jason Warnick - USA Dominic Wright - Sweden Carla Denise Bonan - Brazil Mônica Ryff Moreira Roca Vianna - Brazil Zofia Zukowska - USA Allan Kalueff - USA Georgianna Gould - USA Oliver Braubach - Canada Anderson Manoel Herculano - Brazil David Echevarria - USA Joe Schroeder - USA Jason Warnick - USA Dominic Wright - Sweden Carla Denise Bonan - Brazil Mônica Ryff Moreira Roca Vianna - Brazil Zofia Zukowska - USA

4. The main goal of ZNRC is to promote zebrafish neuroscience research. The main goal of ZNRC is to promote zebrafish neuroscience research. Created in February 2010, ZNRC offers excellent networking opportunities and peer support for active zebrafish labs. Created in February 2010, ZNRC offers excellent networking opportunities and peer support for active zebrafish labs.

5. ZND Zebrafish Neurophenome Database Goals: Goals: Pioneering Pioneering Accessible Accessible Easy to use Easy to use Useful Useful

6. Emerging Model Organism The Zebrafish is an emerging model animal for vertebrate development and neurological function The Zebrafish is an emerging model animal for vertebrate development and neurological function Offers a good compliment to other animal models Offers a good compliment to other animal models Advantages Advantages Easy to take care of Easy to take care of Inexpensive Inexpensive Easy to test Easy to test Quantifiable behavioral endpoints Quantifiable behavioral endpoints

7. Inspiration Mice Mice Mouse Genome Informatics (G+P) Mouse Phenome Database (P) Rats Rats Rat Genome Database (G) National BioResource Project (P) Zebrafish Zebrafish ZFIN (Genome) NCBI (Genome) The point? A Phenome database for the Zebrafish does not yet exist, but inevitably will sometime soon

8. PubMed search “zebrafish” 1900-1985 - 61 papers 1985-1990 - 114 papers 1990-1995 - 487 papers 1995-2000 - 1981 papers 2000-2005 - 4946 papers 2005-2010 - 7538 papers Between 2005 and 2010 the total number of zebrafish-related papers ever written doubled.

9. ZND Zebrafish Neurophenome Database Goals: Goals: Pioneering (so far) Pioneering (so far) Accessible Accessible Easy to use Easy to use Useful Useful

10. www.kaluefflab.com/znpindex -or- www.tulane.edu/~znpindex/search

11. ZND Zebrafish Neurophenome Database Goals: Goals: Pioneering (so far) Pioneering (so far) Accessible (the internet) Accessible (the internet) Easy to use Easy to use Useful Useful

12. What we see, Not easy

13. What you’ll see

16. Everything you need to know… Hopefully

17. If not.. Submit your data! Submit your data! More on this later More on this later

18. How it’s organized Experiment 1 Interval 1.1 Treatment 1.1.1 Interval 1.2Interval 1.3 Treatment 1.1.2 Parent/Child Format

19. Caffeine L/D Box 15 min 100 mg/kg I.P. 250 mg/kg I.P. Chronic Acute withdrawal Experiment Interval Treatment

20. that’s what you’re seeing here Experiment Interval Treatment Test Results

21. ZND Zebrafish Neurophenome Database Goals: Goals: Pioneering (so far) Pioneering (so far) Accessible (the internet) Accessible (the internet) Easy to use (we hope so) Easy to use (we hope so) Useful Useful

22. Only With Your Help Submit your data! (2 methods) Submit your data! (2 methods) 1. Direct info submission with the submission template (next slide) 2. Send us a paper (please use step #1)

23. Submission Template

24. If you send us a paper…

25. We fill out the template

26. ZND Zebrafish Neurophenome Database Goals: Goals: Pioneering (so far) Pioneering (so far) Accessible (the internet) Accessible (the internet) Easy to use (we hope so) Easy to use (we hope so) Useful (with your help) Useful (with your help)

27. Special Thanks Budd Hirons - who has been essential to the actualization of this idea. Budd Hirons - who has been essential to the actualization of this idea. ILC (Innovative Learning Center) & Tulane University Technology Services ILC (Innovative Learning Center) & Tulane University Technology Services Also, Tulane has been kind enough to supply us with the server space that is required to host such a database. Also, Tulane has been kind enough to supply us with the server space that is required to host such a database.

28. In Conclusion The Zebrafish is an emerging animal model for vertebrate development and neurological function The Zebrafish is an emerging animal model for vertebrate development and neurological function We hope to work closely with other zebrafish-based databases to help provide a better, more comprehensive user experience We hope to work closely with other zebrafish-based databases to help provide a better, more comprehensive user experience Check in regularly at: Check in regularly at: http://www.tulane.edu/~znpindex/search http://www.tulane.edu/~znpindex/search http://www.tulane.edu/~znpindex/search Or just search ZNP or Zebrafish Phenome Or just search ZNP or Zebrafish Phenome You can help by submitting your data. You can help by submitting your data.