1. Bioengineering: Engineering Applications For The Real World CU Science Discovery School and Teacher Programs

2. Bioengineering is: the biological and medical applications of engineering principles, practices, tools and technologies

3. Bioengineering at CU BioastronauticsChemical and Biological Biomedical EngineeringEnvironmental Engineering

4. Three Activities: Design a Custom Bacteria Protect That Pill Alginate Encapsulation Bioengineering Practice using engineering design process models in bioscience applications from the real world 75 Minutes of Activities

5. Design Process Models Engineering is Elementary Design Process Model

6. Bacteria Taxonomy

7. Bacteria Name: Escherichia coli Pronunciation: esh-er-i-kia coal-i Abbreviation: E. Coli Bacteria Raw Materials Environments: Warm fresh water with temperature limited to 10 to 40 C (50-104 F) Applications: Sewage, Biological Waste and Simple Organic Compounds E. coli Mobile with Flagella or tails E. coli Capsule has pila that allow it to form bio-films E. coli Genetic option to create non- reproducing strains E. coli Energy from organic compounds E. coli Reproduction is rapid with option for sterile strains E. coli Has Pathology, some can cause diseases in people E. coli Membrane is sensitive to salts, metals & chemicals

8. Bacteria Name: Dechloromonas aromatica Pronunciation: De-cloro-moan-us aro-mat-ica Abbreviation: D. aromatic Bacteria Raw Materials D. aromatica Limited Mobility with Flagella/Tails D. aromatica Capsule cannot tolerate any oxygen D. aromatica No special Genetic features D. aromatica Energy from benzene and perchlorates D. aromatica Reproduction is very slow and limited D. aromatica Has no Pathology, not disease causing D. aromatica Cell Membrane is salt tolerant Environments: Soils, deep water and oxygen free environments with average temperatures of 1-32 C (33-90 F) Applications: Perchlorates (salts used in industry/rocket fuel) and Benzene (a persistent industrial pollutant)

9. Bacteria Name: Pseudomonas putida Pronunciation: Su-doe-mon-as pu-tee-da Abbreviation: P. putida Bacteria Raw Materials Environments: Warm, very well oxygenated salt or fresh water from 20 to 35 C (68-95 F) Applications: Oil, plastics and oil-based pesticides (Atrazine) P. putida Full Mobility with Flagella or tails P. putida Cell Wall is rigid and resistant to high pressures P. putida No special Genetic features P. putida Breaks down oil and plastics for Energy P. putida Reproduction is rapid in limited environments P. putida Has Pathology, some cause diseases in people P. putida Cell Membrane is very salt and oil tolerant-resistant

10. Bacteria Name: Nitrosamonas europea Pronunciation: Nitro-samon-as euro-pea Abbreviation: N. europea Bacteria Raw Materials Environments: Soils, sewage and fresh water but with very limited temperature ranges of 20- 30 C (68-86 F) Applications: Ammonia and ammonia-based compounds (fertilizers) N. europea No Mobility N. europea Cell Wall is non- rigid and sensitive to high pressure N. europea Genetic option to limit reproduction to 5 life cycles N. europea Breaks down ammonia for Energy N. europea Reproduction is very slow and can be limited N. europea Has no Pathology, not disease causing N. europea Cell Membrane is resistant to nitrogen & organics

11. Bacteria Name: Deinococcus radiodurans Pronunciation: Dee-no-coch-us radio-dur-ans Abbreviation: D. radiodurans Bacteria Raw Materials Environments: Soils, fresh water, extreme temperatures (20- 55 C), dry environments but not salts or salt water Applications: Radioactive Waste, Heavy Metals and Mercury D. radiodurans No Mobility D. radiodurans Capsule resists heat, cold, light, radiation, but not salts D. radiodurans No special Genetic features D. radiodurans Energy from organic and some inorganic chemicals D. radiodurans Reproduction is rapid and in extreme environments D. radiodurans Has no Pathology, not disease causing D. radiodurans Cell Membrane resists dehydration & chemicals

12. Narrative: We have a crisis that could make or break a small business in rural Colorado. A small-scale processor of organic foods has found pesticides, specifically Atrazine, in a shipment of soybeans they have already started to process! Their grinding equipment needs to be cleaned up fast so they can get back to work but they cannot just wash the atrazine down the drain. The grinders they used are in a very cold warehouse and have a lot of ‘nooks and crannies” so they need a mobile organism, but not one that can ‘get away’. Most importantly, we have to guarantee that the organism will not stick around after the treatment, cause diseases, or get into the food system! Design Challenge #1

13. Design Considerations and Environment: Grinding equipment is contaminated with Atrazine (an oil-based pesticide) They need limited mobility to get into the small spaces, but not one that can get away! The warehouse is very cold and has bright lights (high UV light) The soybeans left behind organic chemicals and Nitrogen compounds The organisms need to be able to get their energy from organic compounds Organisms must NOT be able to survive, even for a day, after treatment The organisms cannot cause diseases in people

14. 3-E Engineering Effective: Will the proposed solution work well and does it meet ALL of the proposed design specifications? Economic: Is the proposed solution done in a way that maximizes product efficiency and return on investment? Ethical: Can the proposed solution cause any harm to people or systems in the long and short term?

15. Design Process Models Engineering is Elementary Design Process Model

16. Design Process Models Engineering is Elementary Design Process Model Insert Design Process Model Images from: www.engr.ncsu.edu

17. 3-E Engineering Effective: Will the proposed solution work well and does it meet ALL of the proposed design specifications? Economic: Is the proposed solution done in a way that maximizes product efficiency and return on investment? Ethical: Can the proposed solution cause any harm to people or systems in the long and short term?

19. Component# of SpoonfulsFraction Water 11/5 Oil 1 1/5 Flour 3 3/5 Corn Starch 0 0/5 Salt 0 0/5 Sugar 0 0/5 Total 5 5/5ths

20. Alginate Encapsulation Polymers are chemical compounds made from repeating chains or networks of smaller compounds called monomers

21. Alginate Encapsulation Insert Images From: http://openwetware.org/wiki/IGEM:IMPERIAL/ 2009/Encapsulation/Phase2/Alginate_Properti es

22. Bioengineering is: the biological and medical applications of engineering principles, practices, tools and technologies

23. Bioengineering at CU BioastronauticsChemical and Biological Biomedical EngineeringEnvironmental Engineering

24. Bioengineering: Engineering Applications From The Real World CU Science Discovery School and Teacher Programs