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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Macromolecular assemblies in DNA- associated functions DNA structures: Chromatin (nucleosome) Replication complexes: Initiation, progression Transcription complexes: Initiation, splicing, progression Other complexes: Repair, recombination December 23, 2004 TIGP-CBMB Molecular biophysics I
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 29-1aStructure of B-DNA. (a) Ball and stick drawing and corresponding space-filling model viewed perpendicular to the helix axis. Page 1108
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 29-21Toroidal and interwound supercoils. Page 1124
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 29-22Sedimentation rate of underwound closed circular duplex DNA as a function of ethidium bromide concentration. Page 1125
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 29-23X-Ray structure of a complex of ethidium with 5- iodo-UpA. Page 1125 Figure 31-17X-Ray structure of actinomycin D in complex with a duplex DNA of self- complementary sequence d(GAAGCTTC).
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 29-26X-Ray structure of the Y328F mutant of E. coli topoisomerase III, a type IA topoisomerase, in complex with the single-stranded octanucleotide d(CGCAACTT). Page 1127
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 29-27Proposed mechanism for the strand passage reaction catalyzed by type IA topoisomerases. Page 1128
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 29-28X-Ray structure of the N-terminally truncated, Y723F mutant of human topoisomerase I in complex with a 22-bp duplex DNA. Page 1129
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 29-31a Structures of topoisomerase II. (a) X-Ray structure of the 92-kD segment of the yeast topoisomerase II (residues 410–1202) dimer. Page 1131
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 29-32Model for the enzymatic mechanism of type II topoisomerases. Page 1131
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 34-1 Electron micrograph of a human metaphase chromosome and of D. melanogaster chromatin showing that its 10-nm fibers are strings of closely spaced nucleosomes. Page 1423
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 34-7aX-Ray structure of the nucleosome core particle. (a) The entire core particle as viewed (left) along its superhelical axis and (right) rotated 90° about the vertical axis. Page 1426
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 34-8X-Ray structure of a histone octamer within the nucleosome core particle. Page 1427 Figure 34-3 The amino acid sequence of calf thymus histone H4. This 102-residue protein’s 25 Arg and Lys residues are indicated in red.
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 34-9Model of the interaction of histone H1 with the DNA of the 166-bp chromatosome. Page 1427
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 34-10 Electron micrographs of chromatin. (a) H1-containing chromatin and (b) H1-depleted chromatin, both in 5 to 15 mM salt. Page 1428 Figure 34-13 Model of the 30-nm chromatin filament. The filament is represented (bottom to top) as it might form with increasing salt concentration.
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Macromolecular assemblies in DNA- associated functions DNA structures: Chromatin (nucleosome) Replication complexes: Initiation, progression Transcription complexes: Initiation, splicing, progression Other complexes: Repair, recombination December 23, 2004 TIGP-CBMB Molecular biophysics I
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 30-1 Action of DNA polymerase. DNA polymerases assemble incoming deoxynucleoside triphosphates on single-stranded DNA templates such that the growing strand is elongated in its 5 3 direction. Page 1137 Figure 30-2Autoradiogram and its interpretive drawing of a replicating E. coli chromosome.
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 30-28The replication of E. coli DNA. Page 1155
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 30-5Semidiscontinuous DNA replication. In DNA replication, both daughter strands (leading strand red, lagging strand blue) are synthesized in their 5 3 directions. Page 1138
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Table 30-1Properties of E. coli DNA Polymerases. Page 1145
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 30-8aX-Ray structure of E. coli DNA polymerase I Klenow fragment (KF) in complex with a dsDNA. Page 1141
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 30-9bX-Ray structure of Klentaq1 in complex with DNA and ddCTP. (a) The closed conformation. (b) The open conformation. Page 1142
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 30-13aX-Ray structure of the subunit of E. coli Pol III holoenzyme. Ribbon drawing. Page 1146
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Table 30-3Unwinding and Binding Proteins of E. coli DNA Replication. Page 1146
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 30-14Unwinding of DNA by the combined action of DnaB and SSB proteins. Page 1147
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Table 30-4Proteins of the Primosome a. Page 1152
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 30-15Electron microscopy–based image reconstruction of T7 gene 4 helicase/primase. Page 1147 X-Ray structure of the helicase domain of T7 gene 4 helicase/primase.
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 30-19X-Ray structure of the N-terminal 135 residues of E. coli SSB in complex with dC(pC) 34. Page 1149
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 30-22X-Ray structure of E. coli primase. Page 1151 Figure 30-25 Electron micrograph of a primosome bound to a fX174 RF I DNA. Such complexes always contain a single primosome with one or two associated small DNA loops.
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 30-23 The synthesis of the M13 (–) strand DNA on a (+) strand template to form M13 RF I DNA. Page 1152 Figure 30-27 The synthesis of the fX174 (+) strand by the looped rolling circle mode.
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 30-29A model for DNA replication initiation at oriC. Page 1156
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Table 30-2Components of E. coli DNA Polymerase III Holoenzyme. Page 1145
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 30-32X-Ray structure of the – complex. Page 1158
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 30-33X-Ray structure of the 3 clamp loading complex. Page 1159
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 30-34Schematic diagram of the clamp loading cycle. This speculative model is based on a combination of structural and biochemical information. Page 1159
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 30-39X-Ray structure of RB69 DNA polymerase (RB69 pol) in complex with primer–template DNA and dTTP. Page 1164
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Macromolecular assemblies in DNA- associated functions DNA structures: Chromatin (nucleosome) Replication complexes: Initiation, progression Transcription complexes: Initiation, splicing, progression Other complexes: Repair, recombination December 23, 2004 TIGP-CBMB Molecular biophysics I
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 34-42Immunofluorescence micrograph of a lampbrush chromosome from an oocyte nucleus of the newt Notophthalmus viridescens. Page 1449
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 34-47Assembly of the preinitiation complex (PIC) on a TATA box–containing promoter. Page 1452
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 34-48aX-Ray structure of Arabidopsis thaliana TATA box– binding protein (TBP). (a) A ribbon diagram of the protein in the absence of DNA. (b) TBP with a 14-bp TATA box–containing segment DNA. Page 1453
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 34-49Model of the TFIIA–TFIIB–TBP–TATA box– containing DNA quaternary complex. Page 1454
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 34-50EM-based image of the human TFIID– TFIIA–TFIIB complex at 35-Å resolution. Page 1454
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 31-9An electron micrograph of E. coli RNA polymerase (RNAP) holoenzyme attached to various promoter sites on bacteriophage T7 DNA. Page 1222
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 31-10The sense (nontemplate) strand sequences of selected E. coli promoters. Page 1223
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 31-11aX-Ray structure of Taq RNAP core enzyme. subunits are yellow and green, subunit is cyan, subunit is pink, subunit is gray. (b) The holoenzyme viewed as in Part a. Page 1224
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 31-21bX-Ray structure of an RNAP II elongation complex. Page 1234
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 31-47The sequence of steps in the production of mature eukaryotic mRNA as shown for the chicken ovalbumin gene. The consensus sequence at the exon–intron junctions of vertebrate pre- mRNAs. Page 1258
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 31-49The sequence of transesterification reactions that splice together the exons of eukaryotic pre-mRNAs. Page 1259
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 31-51aThe self-splicing group I intron from Tetrahymena thermophila. (a) The secondary structure of the entire 413-nt intron. (b) The X-ray structure of P4-P6 viewed as in Part a. Page 1261
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 31-56An electron micrograph of spliceosomes in action. Page 1265
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 31-57A schematic diagram of six rearrangements that the spliceosome undergoes in mediating the first transesterification reaction in pre-mRNA splicing. Page 1265
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 31-60A model of the snRNP core protein. Page 1267
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 31-61aThe electron microscopy-based structure of U1-snRNP at 10 Å resolution. (a) The predicted secondary structure of U1-snRNA. (b) The molecular outline of U1-snRNP. (c) The U1-snRNA colored as in Part a. Page 1268
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Macromolecular assemblies in DNA- associated functions DNA structures: Chromatin (nucleosome) Replication complexes: Initiation, progression Transcription complexes: Initiation, splicing, progression Other complexes: Repair, recombination December 23, 2004 TIGP-CBMB Molecular biophysics I
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 30-54b The structure of E. coli Ada protein. (a) The X-ray structure of Ada’s 178-residue C-terminal segment, which contains its O 6 - alkylguanine–DNA alkyltransferase function.(b) The NMR structure of Ada’s 92-residue, N-terminal segment, which mediates its methyl phosphotriester repair function. Page 1175
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 30-55The mechanism of nucleotide excision repair (NER) of pyrimidine photodimers. Page 1176
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 30-57 X-Ray structure of human uracil–DNA glycosylase (UDG) in complex with a 10-bp DNA containing a U·G base pair. Page 1178 Figure 30-55 The mechanism of nucleotide excision repair (NER) of pyrimidine photodimers.
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 30-64The Holliday model of homologous recombination between homologous DNA duplexes. Page 1184
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 30-67a Electron micrographs of intermediates in the homologous recombination of two plasmids. (a) A figure-8 structure. This corresponds to Fig. 30-66d. (b) A chi structure that results from the treatment of a figure-8 structure with a restriction endonuclease. Page 1186 Figure 30-66 Homologous recombination between two circular DNA duplexes. This process can result either in two circles of the original sizes or in a single composite circle.
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 30-68An electron microscopy–based image (transparent surface) of an E. coli RecA–dsDNA–ATP filament. Page 1187
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 30-71The RecA-catalyzed assimilation of a single- stranded circle by a dsDNA can occur only if the dsDNA has a 3 end that can base pair with the circle (red strand). Page 1188
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 30-72A hypothetical model for the RecA-mediated strand exchange reaction. Page 1189
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 30-75aProposed structure of the T. thermophilus RuvB hexamer. (a) EM image reconstruction of RuvB complexed with DNA (not visible). Page 1191
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 30-76Model of the RuvAB–Holliday junction complex. The model is based on electron micrographs such as that in the inset. Page 1191
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Voet Biochemistry 3e © 2004 John Wiley & Sons, Inc. Figure 34-117aCryoelectron microscopy–based images of the apoptosome at 27-Å resolution. (a) The free apoptosome. (b) The apoptosome in complex with a noncleavable mutant of procaspase-9 in oblique top view. Page 1513
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