1. Bryan Addeman April 6, 2010

2.  Background Theory  Motivation  Measurements  Moving Forward

3.  Label specific metabolites with 13 C  12 C isotope is not magnetically active

5.  Natural abundance of 13 C is ≈ 1.11%  Amount of signal is related to gyromagnetic ratio (γ) & abundance ◦ γ H = 42.58 (Mhz/T) ◦ γ 13C = 10.71 (Mhz/T)  Hyperpolarization can give a factor of 10,000x more initial signal

6.  Inject sample mixed with free radical  Cool to 1.4 K in a High magnetic field  Bombard sample with microwaves  Mix with heated solution  Duration: 1.5 hours  Amount of Hyperpolarization decays with time (T1) Robarts DNP Hyperpolarizer

7. Spin-Spin RelaxationSpin-Lattice Relaxation T1T2

9. Effects of low magnetic fields on T1values for hyperpolarized 13 C Pyruvic Acid

10.  Hyperpolarized sample transport: ◦ B o : High (DNP) -> Low -> High (MRI)  What happens to the sample in transit?  T1 relaxation is a characteristic of the molecule that can be measured

11. SpinmasterOxford Instruments MQC  Variable magnetic field ◦ B o = 0.0-0.9 T  Desktop NMR system ◦ B o = 0.55 T  Both devices can measure Signal amplitude at time intervals

12. B o =0.55, 13 C enriched Pyruvic Acid  Fit Equation  Non-Linear Least Squares Fit

13. SpinmasterMQC (0.55 Static Field) Field Strength (T) T1 (s)Fit Error (s)T1 (s)Fit Error (s) 0.5552.60.4458.30.31 0.023568.70.2587.00.82 0.023574.20.7883.70.56

14.  Need to take more measurements to understand the effects low field strengths on T1 values

15. Supervisors  Dr. Charles McKenzie  Dr. Lanette Friesen- Walder  Dr. Tim Scholl  Dr. Jake Onwuachi Lab  Curtis Wiens  Samantha Flood  Steven Yange  Elmer Soto  Trevor Wade