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Bryan Addeman April 6, 2010

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Background Theory Motivation Measurements Moving Forward

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Label specific metabolites with 13 C 12 C isotope is not magnetically active

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Natural abundance of 13 C is ≈ 1.11% Amount of signal is related to gyromagnetic ratio (γ) & abundance ◦ γ H = 42.58 (Mhz/T) ◦ γ 13C = 10.71 (Mhz/T) Hyperpolarization can give a factor of 10,000x more initial signal

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Inject sample mixed with free radical Cool to 1.4 K in a High magnetic field Bombard sample with microwaves Mix with heated solution Duration: 1.5 hours Amount of Hyperpolarization decays with time (T1) Robarts DNP Hyperpolarizer

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Spin-Spin RelaxationSpin-Lattice Relaxation T1T2

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Effects of low magnetic fields on T1values for hyperpolarized 13 C Pyruvic Acid

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Hyperpolarized sample transport: ◦ B o : High (DNP) -> Low -> High (MRI) What happens to the sample in transit? T1 relaxation is a characteristic of the molecule that can be measured

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SpinmasterOxford Instruments MQC Variable magnetic field ◦ B o = 0.0-0.9 T Desktop NMR system ◦ B o = 0.55 T Both devices can measure Signal amplitude at time intervals

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B o =0.55, 13 C enriched Pyruvic Acid Fit Equation Non-Linear Least Squares Fit

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SpinmasterMQC (0.55 Static Field) Field Strength (T) T1 (s)Fit Error (s)T1 (s)Fit Error (s) 0.5552.60.4458.30.31 0.023568.70.2587.00.82 0.023574.20.7883.70.56

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Need to take more measurements to understand the effects low field strengths on T1 values

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Supervisors Dr. Charles McKenzie Dr. Lanette Friesen- Walder Dr. Tim Scholl Dr. Jake Onwuachi Lab Curtis Wiens Samantha Flood Steven Yange Elmer Soto Trevor Wade

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