9 4. Clinical picture & Social Impact Gut ProblemsAbsorptive DifficultiesEnzyme DisordersDeficiency SyndromesPoor Detoxification AbilitiesImmune ReactivityAllergiesPoor Blood Flow To The BrainHippocampus MalfunctionSocial Impact:Communication ProblemsCognitive DifficultiesEmotional DisordersProblems With AwarenessPoor Eye ContactBehavior ProblemsProblems with PlayPoor Understanding Of Cause and EffectDelayed DevelopmentSleep ProblemsThe DAN approach to treating autism is a biological approach that works hand-in-hand with all the other types of therapies.For those of us who have been involved with DAN, many catchwords keep appearing and reappearing.
11 5. Biochemical & Dietary profile? Autism Research in Oman:Folate and Vitamin B12.Oxidative Stress and Neuronal Cells Damage.Glutathione (GSH), Intracellular Antioxidant.Reduced Methylation Capacity in Autistic Children.Neurodevelopmental Toxicity and GSH Depletion.
12 SOURCES OF OXYGEN FREE RADICALS AND CELL INJURY = Oxidative Stress
13 MECHANISMS OF FREE RADICAL-MEDIATED CELLULAR INJURY Lipid peroxidation – implications for increased permeability in gut and BBB
16 Folate and Vitamin B12-dependent HCY- Methionine Cycle
17 Folate and Vitamin B12 Status Control Children Autistic Children(n=30) (n=30)Serum Folate (µg/l) *Serum B12 (pg/ml) *Dietary Folate (µg/Day) *Diteary B12 (µg/Day) *Results are Mean ± SD,* Significantly lower than control children, P<0.05
18 Oxidative Stress Markers Control Children Autistic Children(n=30) (n=30)Homocysteine , HCY(µmol/l) #Glutathione, GSH (µmol/l) *Total Antioxidant Capacity *TAC, (mmol/l)Results are Mean ± SD,# Significantly higher than control children, P<0.05* Significantly lower than control children, P<0.05
19 6. Genetics? twin studies --- 90%; multiple genes in chromosomes 2 q , 7 q, 16 p, 19 p.Chromosomal anomalies 15 and fragile X syndrome.Recurrence risk studies- siblings of autistic people are more likely to be autistic.Our journey through METHYLCAL SCHOOL begins here with the DAN brochure.
20 Acknowledgment Dr. Marwan Al-Sharabati, Behavioral Medicine Dept. Dr. Mohammed Al-Shafaee, Family Medicine& Public Health Dept.Dr. Yahya Al-Farsi, Family Medicine& public health Dept.Dr. Amanat Ali, Food Science and Nutrition Dept.Dr. Majeed Al-Ani, Food Science and Nutrition Dept.
21 They Disserve Our Effort!! شكراً/I am currently treating between 150 to 200 children with methyl-B12 injections and I receive letters just like these every day!